Imaging and Quantitation Techniques for Tracking Cargo along Endosome-to-Golgi Transport Pathways

Recent improvements in the resolution of light microscopy, coupled with the development of a range of fluorescent-based probes, have provided new approaches to dissecting membrane domains and the regulation of membrane trafficking. Here, we review these advances, as well as highlight developments in...

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Detalles Bibliográficos
Autores principales: Chia, Pei Zhi Cheryl, Gleeson, Paul A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2013
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3972656/
https://www.ncbi.nlm.nih.gov/pubmed/24709647
http://dx.doi.org/10.3390/cells2010105
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author Chia, Pei Zhi Cheryl
Gleeson, Paul A.
author_facet Chia, Pei Zhi Cheryl
Gleeson, Paul A.
author_sort Chia, Pei Zhi Cheryl
collection PubMed
description Recent improvements in the resolution of light microscopy, coupled with the development of a range of fluorescent-based probes, have provided new approaches to dissecting membrane domains and the regulation of membrane trafficking. Here, we review these advances, as well as highlight developments in quantitative image analysis and novel unbiased analytical approaches to quantitate protein localization. The application of these approaches to endosomal sorting and endosome-to-Golgi transport is discussed.
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spelling pubmed-39726562014-04-07 Imaging and Quantitation Techniques for Tracking Cargo along Endosome-to-Golgi Transport Pathways Chia, Pei Zhi Cheryl Gleeson, Paul A. Cells Review Recent improvements in the resolution of light microscopy, coupled with the development of a range of fluorescent-based probes, have provided new approaches to dissecting membrane domains and the regulation of membrane trafficking. Here, we review these advances, as well as highlight developments in quantitative image analysis and novel unbiased analytical approaches to quantitate protein localization. The application of these approaches to endosomal sorting and endosome-to-Golgi transport is discussed. MDPI 2013-02-22 /pmc/articles/PMC3972656/ /pubmed/24709647 http://dx.doi.org/10.3390/cells2010105 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Review
Chia, Pei Zhi Cheryl
Gleeson, Paul A.
Imaging and Quantitation Techniques for Tracking Cargo along Endosome-to-Golgi Transport Pathways
title Imaging and Quantitation Techniques for Tracking Cargo along Endosome-to-Golgi Transport Pathways
title_full Imaging and Quantitation Techniques for Tracking Cargo along Endosome-to-Golgi Transport Pathways
title_fullStr Imaging and Quantitation Techniques for Tracking Cargo along Endosome-to-Golgi Transport Pathways
title_full_unstemmed Imaging and Quantitation Techniques for Tracking Cargo along Endosome-to-Golgi Transport Pathways
title_short Imaging and Quantitation Techniques for Tracking Cargo along Endosome-to-Golgi Transport Pathways
title_sort imaging and quantitation techniques for tracking cargo along endosome-to-golgi transport pathways
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3972656/
https://www.ncbi.nlm.nih.gov/pubmed/24709647
http://dx.doi.org/10.3390/cells2010105
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