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Functional Assessment of Pharmacological Telomerase Activators in Human T cells

Telomeres are structures at the ends of chromosomes that shorten during cell division and eventually signal an irreversible state of growth arrest known as cellular senescence. To delay this cellular aging, human T cells, which are critical in the immune control over infections and cancer, activate...

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Autores principales: Molgora, Brenda, Bateman, Riley, Sweeney, Greg, Finger, Danielle, Dimler, Taylor, Effros, Rita B., Valenzuela, Hector F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3972662/
https://www.ncbi.nlm.nih.gov/pubmed/24709644
http://dx.doi.org/10.3390/cells2010057
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author Molgora, Brenda
Bateman, Riley
Sweeney, Greg
Finger, Danielle
Dimler, Taylor
Effros, Rita B.
Valenzuela, Hector F.
author_facet Molgora, Brenda
Bateman, Riley
Sweeney, Greg
Finger, Danielle
Dimler, Taylor
Effros, Rita B.
Valenzuela, Hector F.
author_sort Molgora, Brenda
collection PubMed
description Telomeres are structures at the ends of chromosomes that shorten during cell division and eventually signal an irreversible state of growth arrest known as cellular senescence. To delay this cellular aging, human T cells, which are critical in the immune control over infections and cancer, activate the enzyme telomerase, which binds and extends the telomeres. Several different extracts from the Astragalus membranaceus root have been documented to activate telomerase activity in human T cells. The objective of this research was to compare two extracts from Astragalus membranaceus, TA-65 and HTA, for their effects on both telomerase and proliferative activity of human CD4 and CD8 T cells. Our results demonstrate that, TA-65 increased telomerase activity significantly (1.3 to 3.3-fold relative to controls) in T cell cultures from six donors tested, whereas HTA only increased telomerase levels in two out of six donors. We also demonstrate that TA-65 activates telomerase by a MAPK- specific pathway. Finally, we determine that during a three-day culture period, only the T cells treated with the TA-65 extract showed a statistically significant increase in proliferative activity. Our results underscore the importance of comparing multiple telomerase activators within the same experiment, and of including functional assays in addition to measuring telomerase activity.
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spelling pubmed-39726622014-04-07 Functional Assessment of Pharmacological Telomerase Activators in Human T cells Molgora, Brenda Bateman, Riley Sweeney, Greg Finger, Danielle Dimler, Taylor Effros, Rita B. Valenzuela, Hector F. Cells Article Telomeres are structures at the ends of chromosomes that shorten during cell division and eventually signal an irreversible state of growth arrest known as cellular senescence. To delay this cellular aging, human T cells, which are critical in the immune control over infections and cancer, activate the enzyme telomerase, which binds and extends the telomeres. Several different extracts from the Astragalus membranaceus root have been documented to activate telomerase activity in human T cells. The objective of this research was to compare two extracts from Astragalus membranaceus, TA-65 and HTA, for their effects on both telomerase and proliferative activity of human CD4 and CD8 T cells. Our results demonstrate that, TA-65 increased telomerase activity significantly (1.3 to 3.3-fold relative to controls) in T cell cultures from six donors tested, whereas HTA only increased telomerase levels in two out of six donors. We also demonstrate that TA-65 activates telomerase by a MAPK- specific pathway. Finally, we determine that during a three-day culture period, only the T cells treated with the TA-65 extract showed a statistically significant increase in proliferative activity. Our results underscore the importance of comparing multiple telomerase activators within the same experiment, and of including functional assays in addition to measuring telomerase activity. MDPI 2013-01-14 /pmc/articles/PMC3972662/ /pubmed/24709644 http://dx.doi.org/10.3390/cells2010057 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Molgora, Brenda
Bateman, Riley
Sweeney, Greg
Finger, Danielle
Dimler, Taylor
Effros, Rita B.
Valenzuela, Hector F.
Functional Assessment of Pharmacological Telomerase Activators in Human T cells
title Functional Assessment of Pharmacological Telomerase Activators in Human T cells
title_full Functional Assessment of Pharmacological Telomerase Activators in Human T cells
title_fullStr Functional Assessment of Pharmacological Telomerase Activators in Human T cells
title_full_unstemmed Functional Assessment of Pharmacological Telomerase Activators in Human T cells
title_short Functional Assessment of Pharmacological Telomerase Activators in Human T cells
title_sort functional assessment of pharmacological telomerase activators in human t cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3972662/
https://www.ncbi.nlm.nih.gov/pubmed/24709644
http://dx.doi.org/10.3390/cells2010057
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