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CD8+ T Lymphocyte Epitopes From The Herpes Simplex Virus Type 2 ICP27, VP22 and VP13/14 Proteins To Facilitate Vaccine Design And Characterization

CD8+ T cells have the potential to control HSV-2 infection. However, limited information has been available on CD8+ T cell epitopes or the functionality of antigen specific T cells during infection or following immunization with experimental vaccines. Peptide panels from HSV-2 proteins ICP27, VP22 a...

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Autores principales: Platt, Rebecca J., Khodai, Tansi, Townend, Tim J., Bright, Helen H., Cockle, Paul, Perez-Tosar, Luis, Webster, Rob, Champion, Brian, Hickling, Timothy P., Mirza, Fareed
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3972665/
https://www.ncbi.nlm.nih.gov/pubmed/24709642
http://dx.doi.org/10.3390/cells2010019
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author Platt, Rebecca J.
Khodai, Tansi
Townend, Tim J.
Bright, Helen H.
Cockle, Paul
Perez-Tosar, Luis
Webster, Rob
Champion, Brian
Hickling, Timothy P.
Mirza, Fareed
author_facet Platt, Rebecca J.
Khodai, Tansi
Townend, Tim J.
Bright, Helen H.
Cockle, Paul
Perez-Tosar, Luis
Webster, Rob
Champion, Brian
Hickling, Timothy P.
Mirza, Fareed
author_sort Platt, Rebecca J.
collection PubMed
description CD8+ T cells have the potential to control HSV-2 infection. However, limited information has been available on CD8+ T cell epitopes or the functionality of antigen specific T cells during infection or following immunization with experimental vaccines. Peptide panels from HSV-2 proteins ICP27, VP22 and VP13/14 were selected from in silico predictions of binding to human HLA-A*0201 and mouse H-2Kd, Ld and Dd molecules. Nine previously uncharacterized CD8+ T cell epitopes were identified from HSV-2 infected BALB/c mice. HSV-2 specific peptide sequences stabilized HLA-A*02 surface expression with intermediate or high affinity binding. Peptide specific CD8+ human T cell lines from peripheral blood lymphocytes were generated from a HLA-A*02+ donor. High frequencies of peptide specific CD8+ T cell responses were elicited in mice by DNA vaccination with ICP27, VP22 and VP13/14, as demonstrated by CD107a mobilization. Vaccine driven T cell responses displayed a more focused immune response than those induced by viral infection. Furthermore, vaccination with ICP27 reduced viral shedding and reduced the clinical impact of disease. In conclusion, this study describes novel HSV-2 epitopes eliciting strong CD8+ T cell responses that may facilitate epitope based vaccine design and aid immunomonitoring of antigen specific T cell frequencies in preclinical and clinical settings.
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spelling pubmed-39726652014-04-07 CD8+ T Lymphocyte Epitopes From The Herpes Simplex Virus Type 2 ICP27, VP22 and VP13/14 Proteins To Facilitate Vaccine Design And Characterization Platt, Rebecca J. Khodai, Tansi Townend, Tim J. Bright, Helen H. Cockle, Paul Perez-Tosar, Luis Webster, Rob Champion, Brian Hickling, Timothy P. Mirza, Fareed Cells Article CD8+ T cells have the potential to control HSV-2 infection. However, limited information has been available on CD8+ T cell epitopes or the functionality of antigen specific T cells during infection or following immunization with experimental vaccines. Peptide panels from HSV-2 proteins ICP27, VP22 and VP13/14 were selected from in silico predictions of binding to human HLA-A*0201 and mouse H-2Kd, Ld and Dd molecules. Nine previously uncharacterized CD8+ T cell epitopes were identified from HSV-2 infected BALB/c mice. HSV-2 specific peptide sequences stabilized HLA-A*02 surface expression with intermediate or high affinity binding. Peptide specific CD8+ human T cell lines from peripheral blood lymphocytes were generated from a HLA-A*02+ donor. High frequencies of peptide specific CD8+ T cell responses were elicited in mice by DNA vaccination with ICP27, VP22 and VP13/14, as demonstrated by CD107a mobilization. Vaccine driven T cell responses displayed a more focused immune response than those induced by viral infection. Furthermore, vaccination with ICP27 reduced viral shedding and reduced the clinical impact of disease. In conclusion, this study describes novel HSV-2 epitopes eliciting strong CD8+ T cell responses that may facilitate epitope based vaccine design and aid immunomonitoring of antigen specific T cell frequencies in preclinical and clinical settings. MDPI 2013-01-04 /pmc/articles/PMC3972665/ /pubmed/24709642 http://dx.doi.org/10.3390/cells2010019 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Platt, Rebecca J.
Khodai, Tansi
Townend, Tim J.
Bright, Helen H.
Cockle, Paul
Perez-Tosar, Luis
Webster, Rob
Champion, Brian
Hickling, Timothy P.
Mirza, Fareed
CD8+ T Lymphocyte Epitopes From The Herpes Simplex Virus Type 2 ICP27, VP22 and VP13/14 Proteins To Facilitate Vaccine Design And Characterization
title CD8+ T Lymphocyte Epitopes From The Herpes Simplex Virus Type 2 ICP27, VP22 and VP13/14 Proteins To Facilitate Vaccine Design And Characterization
title_full CD8+ T Lymphocyte Epitopes From The Herpes Simplex Virus Type 2 ICP27, VP22 and VP13/14 Proteins To Facilitate Vaccine Design And Characterization
title_fullStr CD8+ T Lymphocyte Epitopes From The Herpes Simplex Virus Type 2 ICP27, VP22 and VP13/14 Proteins To Facilitate Vaccine Design And Characterization
title_full_unstemmed CD8+ T Lymphocyte Epitopes From The Herpes Simplex Virus Type 2 ICP27, VP22 and VP13/14 Proteins To Facilitate Vaccine Design And Characterization
title_short CD8+ T Lymphocyte Epitopes From The Herpes Simplex Virus Type 2 ICP27, VP22 and VP13/14 Proteins To Facilitate Vaccine Design And Characterization
title_sort cd8+ t lymphocyte epitopes from the herpes simplex virus type 2 icp27, vp22 and vp13/14 proteins to facilitate vaccine design and characterization
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3972665/
https://www.ncbi.nlm.nih.gov/pubmed/24709642
http://dx.doi.org/10.3390/cells2010019
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