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Temporal Gene Expression Kinetics for Human Keratinocytes Exposed to Hyperthermic Stress

The gene expression kinetics for human cells exposed to hyperthermic stress are not well characterized. In this study, we identified and characterized the genes that are differentially expressed in human epidermal keratinocyte (HEK) cells exposed to hyperthermic stress. In order to obtain temporal g...

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Autores principales: Echchgadda, Ibtissam, Roth, Caleb C., Cerna, Cesario Z., Wilmink, Gerald J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3972685/
https://www.ncbi.nlm.nih.gov/pubmed/24709698
http://dx.doi.org/10.3390/cells2020224
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author Echchgadda, Ibtissam
Roth, Caleb C.
Cerna, Cesario Z.
Wilmink, Gerald J.
author_facet Echchgadda, Ibtissam
Roth, Caleb C.
Cerna, Cesario Z.
Wilmink, Gerald J.
author_sort Echchgadda, Ibtissam
collection PubMed
description The gene expression kinetics for human cells exposed to hyperthermic stress are not well characterized. In this study, we identified and characterized the genes that are differentially expressed in human epidermal keratinocyte (HEK) cells exposed to hyperthermic stress. In order to obtain temporal gene expression kinetics, we exposed HEK cells to a heat stress protocol (44 °C for 40 min) and used messenger RNA (mRNA) microarrays at 0 h, 4 h and 24 h post-exposure. Bioinformatics software was employed to characterize the chief biological processes and canonical pathways associated with these heat stress genes. The data shows that the genes encoding for heat shock proteins (HSPs) that function to prevent further protein denaturation and aggregation, such as HSP40, HSP70 and HSP105, exhibit maximal expression immediately after exposure to hyperthermic stress. In contrast, the smaller HSPs, such as HSP10 and HSP27, which function in mitochondrial protein biogenesis and cellular adaptation, exhibit maximal expression during the “recovery phase”, roughly 24 h post-exposure. These data suggest that the temporal expression kinetics for each particular HSP appears to correlate with the cellular function that is required at each time point. In summary, these data provide additional insight regarding the expression kinetics of genes that are triggered in HEK cells exposed to hyperthermic stress.
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spelling pubmed-39726852014-04-07 Temporal Gene Expression Kinetics for Human Keratinocytes Exposed to Hyperthermic Stress Echchgadda, Ibtissam Roth, Caleb C. Cerna, Cesario Z. Wilmink, Gerald J. Cells Article The gene expression kinetics for human cells exposed to hyperthermic stress are not well characterized. In this study, we identified and characterized the genes that are differentially expressed in human epidermal keratinocyte (HEK) cells exposed to hyperthermic stress. In order to obtain temporal gene expression kinetics, we exposed HEK cells to a heat stress protocol (44 °C for 40 min) and used messenger RNA (mRNA) microarrays at 0 h, 4 h and 24 h post-exposure. Bioinformatics software was employed to characterize the chief biological processes and canonical pathways associated with these heat stress genes. The data shows that the genes encoding for heat shock proteins (HSPs) that function to prevent further protein denaturation and aggregation, such as HSP40, HSP70 and HSP105, exhibit maximal expression immediately after exposure to hyperthermic stress. In contrast, the smaller HSPs, such as HSP10 and HSP27, which function in mitochondrial protein biogenesis and cellular adaptation, exhibit maximal expression during the “recovery phase”, roughly 24 h post-exposure. These data suggest that the temporal expression kinetics for each particular HSP appears to correlate with the cellular function that is required at each time point. In summary, these data provide additional insight regarding the expression kinetics of genes that are triggered in HEK cells exposed to hyperthermic stress. MDPI 2013-04-10 /pmc/articles/PMC3972685/ /pubmed/24709698 http://dx.doi.org/10.3390/cells2020224 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Echchgadda, Ibtissam
Roth, Caleb C.
Cerna, Cesario Z.
Wilmink, Gerald J.
Temporal Gene Expression Kinetics for Human Keratinocytes Exposed to Hyperthermic Stress
title Temporal Gene Expression Kinetics for Human Keratinocytes Exposed to Hyperthermic Stress
title_full Temporal Gene Expression Kinetics for Human Keratinocytes Exposed to Hyperthermic Stress
title_fullStr Temporal Gene Expression Kinetics for Human Keratinocytes Exposed to Hyperthermic Stress
title_full_unstemmed Temporal Gene Expression Kinetics for Human Keratinocytes Exposed to Hyperthermic Stress
title_short Temporal Gene Expression Kinetics for Human Keratinocytes Exposed to Hyperthermic Stress
title_sort temporal gene expression kinetics for human keratinocytes exposed to hyperthermic stress
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3972685/
https://www.ncbi.nlm.nih.gov/pubmed/24709698
http://dx.doi.org/10.3390/cells2020224
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