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Paraoxonase 3 Activity and The Ratio of Antioxidant to Peroxidation in The Follicular Fluid of Infertile Women

BACKGROUND: Paraoxonase-3 (PON3), as a high density lipoprotein (HDL)-associated lactonase, is capable of preventing the oxidative modification of low density lipoprotein (LDL). PON3 activity in follicular fluid (FF) is three times more than its activity in serum. However, the detailed role of PON3...

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Autores principales: Rashidi, Mohammad-Reza Rashidi, Eisa-Khaje, Jalal, Farzadi, Laya, Darabi, Masoud, Gasemzadeh, Alieh, Shahnazi, Vahideh, Fayezi, Shabnam Fayezi, Mehdizadeh, Amir, Haji Hosseini, Reza, Nouri, Mohammad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royan Institute 2014
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3973164/
https://www.ncbi.nlm.nih.gov/pubmed/24696769
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author Rashidi, Mohammad-Reza Rashidi
Eisa-Khaje, Jalal
Farzadi, Laya
Darabi, Masoud
Gasemzadeh, Alieh
Shahnazi, Vahideh
Fayezi, Shabnam Fayezi
Mehdizadeh, Amir
Haji Hosseini, Reza
Nouri, Mohammad
author_facet Rashidi, Mohammad-Reza Rashidi
Eisa-Khaje, Jalal
Farzadi, Laya
Darabi, Masoud
Gasemzadeh, Alieh
Shahnazi, Vahideh
Fayezi, Shabnam Fayezi
Mehdizadeh, Amir
Haji Hosseini, Reza
Nouri, Mohammad
author_sort Rashidi, Mohammad-Reza Rashidi
collection PubMed
description BACKGROUND: Paraoxonase-3 (PON3), as a high density lipoprotein (HDL)-associated lactonase, is capable of preventing the oxidative modification of low density lipoprotein (LDL). PON3 activity in follicular fluid (FF) is three times more than its activity in serum. However, the detailed role of PON3 in women’s fertility remains unknown. The aim of this study was to investigate the correlation between PON3 activity in the FF of women undergoing assisted reproductive technique (ART), in vitro fertilization (IVF), or intra-cytoplasmic sperm injection (ICSI). MATERIALS AND METHODS: This cross-sectional study consisted of 50 women from couples with male factor infertility (MFI) or with female factor infertility (FFI). The FF samples were obtained during the ART intervention. PON3 activity, HDL cholesterol (HDL C), total antioxidant status (TAS) and the level of malondialdehyde (MDA) were determined. The morphology of the embryo was determined using embryo cell number (ECN) and embryo fragmentation score (EFS). In addition, fertilization rate (FR) was used an oocyte fertilization index. RESULTS: Of 50 women, 20 women belonged to FFI group and the remaining 30 women belonged to MFI group. PON3 activity in FF of women in FFI group was significantly lower (p<0.05) in comparison with corresponding value in MFI group. The value of PON3 activity/MDA in the FFI group was lower than that in MFI group. Moreover, MDA level in the FF of FFI group was significantly higher (p<0.05) than its concentration in MFI group. Meanwhile, no significant difference was found in HDL-C concentration and TAS of both groups. No significant correlation was observed between the ECN and FF biochemical parameters. There was also a negative correlation between FR and MDA (r=-0.42, p=0.02), whereas a positive relation between FR with PON3 activity (r=0.59, p=0.004), HDL-C (r=0.35, p=0.04) and PON3/MDA (r=0.59, p=0.001). CONCLUSION: According to the results of this study, PON3 activity level as a key component of antioxidant system in FF may directly be associated with the success rate of ART and fertilization rate in women.
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spelling pubmed-39731642014-04-02 Paraoxonase 3 Activity and The Ratio of Antioxidant to Peroxidation in The Follicular Fluid of Infertile Women Rashidi, Mohammad-Reza Rashidi Eisa-Khaje, Jalal Farzadi, Laya Darabi, Masoud Gasemzadeh, Alieh Shahnazi, Vahideh Fayezi, Shabnam Fayezi Mehdizadeh, Amir Haji Hosseini, Reza Nouri, Mohammad Int J Fertil Steril Original Article BACKGROUND: Paraoxonase-3 (PON3), as a high density lipoprotein (HDL)-associated lactonase, is capable of preventing the oxidative modification of low density lipoprotein (LDL). PON3 activity in follicular fluid (FF) is three times more than its activity in serum. However, the detailed role of PON3 in women’s fertility remains unknown. The aim of this study was to investigate the correlation between PON3 activity in the FF of women undergoing assisted reproductive technique (ART), in vitro fertilization (IVF), or intra-cytoplasmic sperm injection (ICSI). MATERIALS AND METHODS: This cross-sectional study consisted of 50 women from couples with male factor infertility (MFI) or with female factor infertility (FFI). The FF samples were obtained during the ART intervention. PON3 activity, HDL cholesterol (HDL C), total antioxidant status (TAS) and the level of malondialdehyde (MDA) were determined. The morphology of the embryo was determined using embryo cell number (ECN) and embryo fragmentation score (EFS). In addition, fertilization rate (FR) was used an oocyte fertilization index. RESULTS: Of 50 women, 20 women belonged to FFI group and the remaining 30 women belonged to MFI group. PON3 activity in FF of women in FFI group was significantly lower (p<0.05) in comparison with corresponding value in MFI group. The value of PON3 activity/MDA in the FFI group was lower than that in MFI group. Moreover, MDA level in the FF of FFI group was significantly higher (p<0.05) than its concentration in MFI group. Meanwhile, no significant difference was found in HDL-C concentration and TAS of both groups. No significant correlation was observed between the ECN and FF biochemical parameters. There was also a negative correlation between FR and MDA (r=-0.42, p=0.02), whereas a positive relation between FR with PON3 activity (r=0.59, p=0.004), HDL-C (r=0.35, p=0.04) and PON3/MDA (r=0.59, p=0.001). CONCLUSION: According to the results of this study, PON3 activity level as a key component of antioxidant system in FF may directly be associated with the success rate of ART and fertilization rate in women. Royan Institute 2014 2014-03-09 /pmc/articles/PMC3973164/ /pubmed/24696769 Text en Any use, distribution, reproduction or abstract of this publication in any medium, with the exception of commercial purposes, is permitted provided the original work is properly cited http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Rashidi, Mohammad-Reza Rashidi
Eisa-Khaje, Jalal
Farzadi, Laya
Darabi, Masoud
Gasemzadeh, Alieh
Shahnazi, Vahideh
Fayezi, Shabnam Fayezi
Mehdizadeh, Amir
Haji Hosseini, Reza
Nouri, Mohammad
Paraoxonase 3 Activity and The Ratio of Antioxidant to Peroxidation in The Follicular Fluid of Infertile Women
title Paraoxonase 3 Activity and The Ratio of Antioxidant to Peroxidation in The Follicular Fluid of Infertile Women
title_full Paraoxonase 3 Activity and The Ratio of Antioxidant to Peroxidation in The Follicular Fluid of Infertile Women
title_fullStr Paraoxonase 3 Activity and The Ratio of Antioxidant to Peroxidation in The Follicular Fluid of Infertile Women
title_full_unstemmed Paraoxonase 3 Activity and The Ratio of Antioxidant to Peroxidation in The Follicular Fluid of Infertile Women
title_short Paraoxonase 3 Activity and The Ratio of Antioxidant to Peroxidation in The Follicular Fluid of Infertile Women
title_sort paraoxonase 3 activity and the ratio of antioxidant to peroxidation in the follicular fluid of infertile women
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3973164/
https://www.ncbi.nlm.nih.gov/pubmed/24696769
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