Cargando…

Binary recombinase systems for high-resolution conditional mutagenesis

Conditional mutagenesis using Cre recombinase expressed from tissue specific promoters facilitates analyses of gene function and cell lineage tracing. Here, we describe two novel dual-promoter-driven conditional mutagenesis systems designed for greater accuracy and optimal efficiency of recombinatio...

Descripción completa

Detalles Bibliográficos
Autores principales:	Hermann, Mario, Stillhard, Patrick, Wildner, Hendrik, Seruggia, Davide, Kapp, Viktor, Sánchez-Iranzo, Héctor, Mercader, Nadia, Montoliu, Lluís, Zeilhofer, Hanns Ulrich, Pelczar, Pawel
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2014
Materias:	Nucleic Acid Enzymes
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3973285/ https://www.ncbi.nlm.nih.gov/pubmed/24413561 http://dx.doi.org/10.1093/nar/gkt1361

Ejemplares similares

Zinc-finger recombinase activities in vitro
por: Prorocic, Marko M., et al.
Publicado: (2011)

Requirements for catalysis in the Cre recombinase active site
por: Gibb, Bryan, et al.
Publicado: (2010)

A phage weaponizes a satellite recombinase to subvert viral restriction
por: Nguyen, Maria H T, et al.
Publicado: (2022)

The Hin recombinase assembles a tetrameric protein swivel that exchanges DNA strands
por: Dhar, Gautam, et al.
Publicado: (2009)

Homology-dependent interactions determine the order of strand exchange by IntDOT recombinase
por: Laprise, Jennifer, et al.
Publicado: (2010)

Determinants of adenine-mutagenesis in diversity-generating retroelements
por: Handa, Sumit, et al.
Publicado: (2020)

A randomized library approach to identifying functional lox site domains for the Cre recombinase
por: Sheren, Jamie, et al.
Publicado: (2007)

Control of directionality in the DNA strand-exchange reaction catalysed by the tyrosine recombinase TnpI
por: Vanhooff, Virginie, et al.
Publicado: (2010)

Zinc is essential for high-affinity DNA binding and recombinase activity of φC31 integrase
por: McEwan, Andrew R., et al.
Publicado: (2011)

Engineering of a target site-specific recombinase by a combined evolution- and structure-guided approach
por: Abi-Ghanem, Josephine, et al.
Publicado: (2013)

Understanding how the V(D)J recombinase catalyzes transesterification: distinctions between DNA cleavage and transposition
por: Lu, Catherine P., et al.
Publicado: (2008)

Stimulation of fission yeast and mouse Hop2-Mnd1 of the Dmc1 and Rad51 recombinases
por: Ploquin, Mickaël, et al.
Publicado: (2007)

Restoration of catalytic functions in Cre recombinase mutants by electrostatic compensation between active site and DNA substrate
por: Kachroo, Aashiq H., et al.
Publicado: (2010)

Tumor-associated mutations in a conserved structural motif alter physical and biochemical properties of human RAD51 recombinase
por: Chen, Jianhong, et al.
Publicado: (2015)

Crossover-site sequence and DNA torsional stress control strand interchanges by the Bxb1 site-specific serine recombinase
por: Keenholtz, Ross A., et al.
Publicado: (2016)

High-throughput mutagenesis reveals functional determinants for DNA targeting by activation-induced deaminase
por: Gajula, Kiran S., et al.
Publicado: (2014)

Nicked-site substrates for a serine recombinase reveal enzyme–DNA communications and an essential tethering role of covalent enzyme–DNA linkages
por: Olorunniji, Femi J., et al.
Publicado: (2015)

Rad51 and RecA juxtapose dsDNA ends ready for DNA ligase-catalyzed end-joining under recombinase-suppressive conditions
por: Konomura, Naoto, et al.
Publicado: (2017)

Probing RNA recognition by human ADAR2 using a high-throughput mutagenesis method
por: Wang, Yuru, et al.
Publicado: (2016)

Enzyme cycling contributes to efficient induction of genome mutagenesis by the cytidine deaminase APOBEC3B
por: Adolph, Madison B, et al.
Publicado: (2017)

Random mutagenesis of a hyperthermophilic archaeon identified tRNA modifications associated with cellular hyperthermotolerance
por: Orita, Izumi, et al.
Publicado: (2019)

Real-time single-molecule tethered particle motion analysis reveals mechanistic similarities and contrasts of Flp site-specific recombinase with Cre and λ Int
por: Fan, Hsiu-Fang, et al.
Publicado: (2013)

Single molecule TPM analysis of the catalytic pentad mutants of Cre and Flp site-specific recombinases: contributions of the pentad residues to the pre-chemical steps of recombination
por: Fan, Hsiu-Fang, et al.
Publicado: (2015)

A four-subunit DNA polymerase ζ complex containing Pol δ accessory subunits is essential for PCNA-mediated mutagenesis
por: Makarova, Alena V., et al.
Publicado: (2012)

Insights into the pre-initiation events of bacteriophage φ6 RNA-dependent RNA polymerase: towards the assembly of a productive binary complex
por: Sarin, L. Peter, et al.
Publicado: (2009)

Functional validation of mouse tyrosinase non-coding regulatory DNA elements by CRISPR–Cas9-mediated mutagenesis
por: Seruggia, Davide, et al.
Publicado: (2015)

Mechanistic studies of the modulation of cleavage activity of topoisomerase I by DNA adducts of mono- and bi-functional Pt(II) complexes
por: Malina, Jaroslav, et al.
Publicado: (2009)

Ribonucleotides embedded in template DNA impair mitochondrial RNA polymerase progression
por: Singh, Meenakshi, et al.
Publicado: (2022)

DNA melting by RNA polymerase at the T7A1 promoter precedes the rate-limiting step at 37°C and results in the accumulation of an off-pathway intermediate
por: Rogozina, Anastasia, et al.
Publicado: (2009)

DNA processing by the MOB(H) family relaxase TraI encoded within the gonococcal genetic island
por: Heilers, Jan-Hendrik, et al.
Publicado: (2019)

Substrate conformational dynamics facilitate structure-specific recognition of gapped DNA by DNA polymerase
por: Craggs, Timothy D, et al.
Publicado: (2019)

The eukaryotic leading and lagging strand DNA polymerases are loaded onto primer-ends via separate mechanisms but have comparable processivity in the presence of PCNA
por: Chilkova, Olga, et al.
Publicado: (2007)

Two type I topoisomerases maintain DNA topology in human mitochondria
por: Menger, Katja E, et al.
Publicado: (2022)

Magnesium-binding studies reveal fundamental differences between closely related RNA triphosphatases
por: Soulière, Marie F., et al.
Publicado: (2008)

Mechanisms of covalent self-assembly of the Azoarcus ribozyme from four fragment oligonucleotides
por: Draper, Will E., et al.
Publicado: (2008)

Cordycepin-hypersensitive growth links elevated polyphosphate levels to inhibition of poly(A) polymerase in Saccharomyces cerevisiae
por: Holbein, Sandra, et al.
Publicado: (2008)

The Leu22Pro tumor-associated variant of DNA polymerase beta is dRP lyase deficient
por: Dalal, Shibani, et al.
Publicado: (2008)

DNA polymerase kappa produces interrupted mutations and displays polar pausing within mononucleotide microsatellite sequences
por: Hile, Suzanne E., et al.
Publicado: (2008)

The N-terminal domain of TWINKLE contributes to single-stranded DNA binding and DNA helicase activities
por: Farge, Géraldine, et al.
Publicado: (2008)

The yeast ribosome synthesis factor Emg1 is a novel member of the superfamily of alpha/beta knot fold methyltransferases
por: Leulliot, Nicolas, et al.
Publicado: (2008)

Cannot write session to /tmp/vufind_sessions/sess_e90deho3anb2n3ohjtdet240dq