Cargando…

An arginine-aspartate network in the active site of bacterial TruB is critical for catalyzing pseudouridine formation

Pseudouridine synthases introduce the most common RNA modification and likely use the same catalytic mechanism. Besides a catalytic aspartate residue, the contributions of other residues for catalysis of pseudouridine formation are poorly understood. Here, we have tested the role of a conserved basi...

Descripción completa

Detalles Bibliográficos
Autores principales:	Friedt, Jenna, Leavens, Fern M. V., Mercier, Evan, Wieden, Hans-Joachim, Kothe, Ute
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2014
Materias:	Nucleic Acid Enzymes
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3973310/ https://www.ncbi.nlm.nih.gov/pubmed/24371284 http://dx.doi.org/10.1093/nar/gkt1331

Ejemplares similares

tRNA elbow modifications affect the tRNA pseudouridine synthase TruB and the methyltransferase TrmA
por: Schultz, Sarah Kai-Leigh, et al.
Publicado: (2020)

The human pseudouridine synthase PUS7 recognizes RNA with an extended multi-domain binding surface
por: Guegueniat, Julia, et al.
Publicado: (2021)

The ribosome assembly factor Nep1 responsible for Bowen–Conradi syndrome is a pseudouridine-N1-specific methyltransferase
por: Wurm, Jan Philip, et al.
Publicado: (2010)

Pseudouridine synthase 1: a site-specific synthase without strict sequence recognition requirements
por: Sibert, Bryan S., et al.
Publicado: (2012)

Kinetic and thermodynamic characterization of the reaction pathway of box H/ACA RNA-guided pseudouridine formation
por: Yang, Xinxing, et al.
Publicado: (2012)

Pseudouridine at position 55 in tRNA controls the contents of other modified nucleotides for low-temperature adaptation in the extreme-thermophilic eubacterium Thermus thermophilus
por: Ishida, Kazuo, et al.
Publicado: (2011)

The methyltransferase TrmA facilitates tRNA folding through interaction with its RNA-binding domain
por: Keffer-Wilkes, Laura Carole, et al.
Publicado: (2020)

Critical role of DNA intercalation in enzyme-catalyzed nucleotide flipping
por: Hendershot, Jenna M., et al.
Publicado: (2014)

Experimental demonstration of operon formation catalyzed by insertion sequence
por: Kanai, Yuki, et al.
Publicado: (2022)

Identification of two conserved aspartic acid residues required for DNA digestion by a novel thermophilic Exonuclease VII in Thermotoga maritima
por: Larrea, Andres A., et al.
Publicado: (2008)

Duplex strand joining reactions catalyzed by vaccinia virus DNA polymerase
por: Hamilton, Michael D., et al.
Publicado: (2007)

Nonfilament-forming RecA dimer catalyzes homologous joint formation
por: Shinohara, Takeshi, et al.
Publicado: (2018)

A unique arginine cluster in PolDIP2 enhances nucleotide binding and DNA synthesis by PrimPol
por: Kasho, Kazutoshi, et al.
Publicado: (2021)

High fidelity of RecA-catalyzed recombination: a watchdog of genetic diversity
por: Sagi, Dror, et al.
Publicado: (2006)

Polymerase-catalyzed synthesis of DNA from phosphoramidate conjugates of deoxynucleotides and amino acids
por: Adelfinskaya, O., et al.
Publicado: (2007)

Mechanism of double-base lesion bypass catalyzed by a Y-family DNA polymerase
por: Brown, Jessica A., et al.
Publicado: (2008)

Mechanistic consequences of temperature on DNA polymerization catalyzed by a Y-family DNA polymerase
por: Fiala, Kevin A., et al.
Publicado: (2008)

DNA polymerase proofreading: active site switching catalyzed by the bacteriophage T4 DNA polymerase
por: Fidalgo da Silva, Elizabeth, et al.
Publicado: (2007)

Controlling tetramer formation, subunit rotation and DNA ligation during Hin-catalyzed DNA inversion
por: Chang, Yong, et al.
Publicado: (2015)

Evidence that a catalytic glutamate and an ‘Arginine Toggle’ act in concert to mediate ATP hydrolysis and mechanochemical coupling in a viral DNA packaging motor
por: Ortiz, David, et al.
Publicado: (2019)

Understanding how the V(D)J recombinase catalyzes transesterification: distinctions between DNA cleavage and transposition
por: Lu, Catherine P., et al.
Publicado: (2008)

Archaeal RNA ligase is a homodimeric protein that catalyzes intramolecular ligation of single-stranded RNA and DNA
por: Torchia, Christopher, et al.
Publicado: (2008)

MutT homologue 1 (MTH1) catalyzes the hydrolysis of mutagenic O6-methyl-dGTP
por: Jemth, Ann-Sofie, et al.
Publicado: (2018)

Mutation of the arginine finger in the active site of Escherichia coli DbpA abolishes ATPase and helicase activity and confers a dominant slow growth phenotype
por: Elles, Lisa M. Sharpe, et al.
Publicado: (2008)

Quantitative analysis of the efficiency and mutagenic spectra of abasic lesion bypass catalyzed by human Y-family DNA polymerases
por: Sherrer, Shanen M., et al.
Publicado: (2011)

Formation of m(2)G6 in Methanocaldococcus jannaschii tRNA catalyzed by the novel methyltransferase Trm14
por: Menezes, Sheena, et al.
Publicado: (2011)

DNA gyrase with a single catalytic tyrosine can catalyze DNA supercoiling by a nicking-closing mechanism
por: Gubaev, Airat, et al.
Publicado: (2016)

Methylation at position 32 of tRNA catalyzed by TrmJ alters oxidative stress response in Pseudomonas aeruginosa
por: Jaroensuk, Juthamas, et al.
Publicado: (2016)

Significant contribution of the 3′→5′ exonuclease activity to the high fidelity of nucleotide incorporation catalyzed by human DNA polymerase ϵ
por: Zahurancik, Walter J., et al.
Publicado: (2014)

Evolutionarily conserved proteins MnmE and GidA catalyze the formation of two methyluridine derivatives at tRNA wobble positions
por: Moukadiri, Ismaïl, et al.
Publicado: (2009)

Cooperative motion of a key positively charged residue and metal ions for DNA replication catalyzed by human DNA Polymerase-η
por: Genna, Vito, et al.
Publicado: (2016)

Gyrase containing a single C-terminal domain catalyzes negative supercoiling of DNA by decreasing the linking number in steps of two
por: Stelljes, Jampa Tsedön, et al.
Publicado: (2018)

A Type III-B Cmr effector complex catalyzes the synthesis of cyclic oligoadenylate second messengers by cooperative substrate binding
por: Han, Wenyuan, et al.
Publicado: (2018)

METTL4 catalyzes m(6)Am methylation in U2 snRNA to regulate pre-mRNA splicing
por: Goh, Yeek Teck, et al.
Publicado: (2020)

‘In-line attack’ conformational effect plays a modest role in an enzyme-catalyzed RNA cleavage: a free energy simulation study
por: Min, Donghong, et al.
Publicado: (2007)

Rad51 and RecA juxtapose dsDNA ends ready for DNA ligase-catalyzed end-joining under recombinase-suppressive conditions
por: Konomura, Naoto, et al.
Publicado: (2017)

CLICK-17, a DNA enzyme that harnesses ultra-low concentrations of either Cu(+) or Cu(2+) to catalyze the azide-alkyne ‘click’ reaction in water
por: Liu, Kun, et al.
Publicado: (2020)

G-quadruplex and G-rich sequence stimulate Pif1p-catalyzed downstream duplex DNA unwinding through reducing waiting time at ss/dsDNA junction
por: Zhang, Bo, et al.
Publicado: (2016)

Mechanism of bacterial gene rearrangement: SprA-catalyzed precise DNA recombination and its directionality control by SprB ensure the gene rearrangement and stable expression of spsM during sporulation in Bacillus subtilis
por: Abe, Kimihiro, et al.
Publicado: (2017)

Creating highly specific nucleases by fusion of active restriction endonucleases and catalytically inactive homing endonucleases
por: Fonfara, Ines, et al.
Publicado: (2012)

Cannot write session to /tmp/vufind_sessions/sess_d9d5hv3f1ott9eibfr5t30odig