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Assessment of Oxidative Damage to Proteins and DNA in Urine of Newborn Infants by a Validated UPLC-MS/MS Approach
The assessment of oxidative stress is highly relevant in clinical Perinatology as it is associated to adverse outcomes in newborn infants. This study summarizes results from the validation of an Ultra Performance Liquid Chromatography–tandem Mass Spectrometry (UPLC-MS/MS) method for the simultaneous...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3973548/ https://www.ncbi.nlm.nih.gov/pubmed/24695409 http://dx.doi.org/10.1371/journal.pone.0093703 |
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author | Kuligowski, Julia Torres-Cuevas, Isabel Quintás, Guillermo Rook, Denise van Goudoever, Johannes B. Cubells, Elena Asensi, Miguel Lliso, Isabel Nuñez, Antonio Vento, Máximo Escobar, Javier |
author_facet | Kuligowski, Julia Torres-Cuevas, Isabel Quintás, Guillermo Rook, Denise van Goudoever, Johannes B. Cubells, Elena Asensi, Miguel Lliso, Isabel Nuñez, Antonio Vento, Máximo Escobar, Javier |
author_sort | Kuligowski, Julia |
collection | PubMed |
description | The assessment of oxidative stress is highly relevant in clinical Perinatology as it is associated to adverse outcomes in newborn infants. This study summarizes results from the validation of an Ultra Performance Liquid Chromatography–tandem Mass Spectrometry (UPLC-MS/MS) method for the simultaneous quantification of the urinary concentrations of a set of endogenous biomarkers, capable to provide a valid snapshot of the oxidative stress status applicable in human clinical trials, especially in the field of Perinatology. The set of analytes included are phenylalanine (Phe), para-tyrosine (p-Tyr), ortho-tyrosine (o-Tyr), meta-tyrosine (m-Tyr), 3-NO(2)-tyrosine (3NO(2)-Tyr), 3-Cl-tyrosine (3Cl-Tyr), 2′-deoxyguanosine (2dG) and 8-hydroxy-2′-deoxyguanosine (8OHdG). Following the FDA-based guidelines, appropriate levels of accuracy and precision, as well as adequate levels of sensitivity with limits of detection (LODs) in the low nanomolar (nmol/L) range were confirmed after method validation. The validity of the proposed UPLC-MS/MS method was assessed by analysing urine samples from a clinical trial in extremely low birth weight (ELBW) infants randomized to be resuscitated with two different initial inspiratory fractions of oxygen. |
format | Online Article Text |
id | pubmed-3973548 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-39735482014-04-04 Assessment of Oxidative Damage to Proteins and DNA in Urine of Newborn Infants by a Validated UPLC-MS/MS Approach Kuligowski, Julia Torres-Cuevas, Isabel Quintás, Guillermo Rook, Denise van Goudoever, Johannes B. Cubells, Elena Asensi, Miguel Lliso, Isabel Nuñez, Antonio Vento, Máximo Escobar, Javier PLoS One Research Article The assessment of oxidative stress is highly relevant in clinical Perinatology as it is associated to adverse outcomes in newborn infants. This study summarizes results from the validation of an Ultra Performance Liquid Chromatography–tandem Mass Spectrometry (UPLC-MS/MS) method for the simultaneous quantification of the urinary concentrations of a set of endogenous biomarkers, capable to provide a valid snapshot of the oxidative stress status applicable in human clinical trials, especially in the field of Perinatology. The set of analytes included are phenylalanine (Phe), para-tyrosine (p-Tyr), ortho-tyrosine (o-Tyr), meta-tyrosine (m-Tyr), 3-NO(2)-tyrosine (3NO(2)-Tyr), 3-Cl-tyrosine (3Cl-Tyr), 2′-deoxyguanosine (2dG) and 8-hydroxy-2′-deoxyguanosine (8OHdG). Following the FDA-based guidelines, appropriate levels of accuracy and precision, as well as adequate levels of sensitivity with limits of detection (LODs) in the low nanomolar (nmol/L) range were confirmed after method validation. The validity of the proposed UPLC-MS/MS method was assessed by analysing urine samples from a clinical trial in extremely low birth weight (ELBW) infants randomized to be resuscitated with two different initial inspiratory fractions of oxygen. Public Library of Science 2014-04-02 /pmc/articles/PMC3973548/ /pubmed/24695409 http://dx.doi.org/10.1371/journal.pone.0093703 Text en © 2014 Kuligowski et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Kuligowski, Julia Torres-Cuevas, Isabel Quintás, Guillermo Rook, Denise van Goudoever, Johannes B. Cubells, Elena Asensi, Miguel Lliso, Isabel Nuñez, Antonio Vento, Máximo Escobar, Javier Assessment of Oxidative Damage to Proteins and DNA in Urine of Newborn Infants by a Validated UPLC-MS/MS Approach |
title | Assessment of Oxidative Damage to Proteins and DNA in Urine of Newborn Infants by a Validated UPLC-MS/MS Approach |
title_full | Assessment of Oxidative Damage to Proteins and DNA in Urine of Newborn Infants by a Validated UPLC-MS/MS Approach |
title_fullStr | Assessment of Oxidative Damage to Proteins and DNA in Urine of Newborn Infants by a Validated UPLC-MS/MS Approach |
title_full_unstemmed | Assessment of Oxidative Damage to Proteins and DNA in Urine of Newborn Infants by a Validated UPLC-MS/MS Approach |
title_short | Assessment of Oxidative Damage to Proteins and DNA in Urine of Newborn Infants by a Validated UPLC-MS/MS Approach |
title_sort | assessment of oxidative damage to proteins and dna in urine of newborn infants by a validated uplc-ms/ms approach |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3973548/ https://www.ncbi.nlm.nih.gov/pubmed/24695409 http://dx.doi.org/10.1371/journal.pone.0093703 |
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