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Cerebrospinal Fluid Biomarker Candidates Associated with Human WNV Neuroinvasive Disease
During the last decade, the epidemiology of WNV in humans has changed in the southern regions of Europe, with high incidence of West Nile fever (WNF) cases, but also of West Nile neuroinvasive disease (WNND). The lack of human vaccine or specific treatment against WNV infection imparts a pressing ne...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3973578/ https://www.ncbi.nlm.nih.gov/pubmed/24695528 http://dx.doi.org/10.1371/journal.pone.0093637 |
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author | Fraisier, Christophe Papa, Anna Granjeaud, Samuel Hintzen, Rogier Martina, Byron Camoin, Luc Almeras, Lionel |
author_facet | Fraisier, Christophe Papa, Anna Granjeaud, Samuel Hintzen, Rogier Martina, Byron Camoin, Luc Almeras, Lionel |
author_sort | Fraisier, Christophe |
collection | PubMed |
description | During the last decade, the epidemiology of WNV in humans has changed in the southern regions of Europe, with high incidence of West Nile fever (WNF) cases, but also of West Nile neuroinvasive disease (WNND). The lack of human vaccine or specific treatment against WNV infection imparts a pressing need to characterize indicators associated with neurological involvement. By its intimacy with central nervous system (CNS) structures, modifications in the cerebrospinal fluid (CSF) composition could accurately reflect CNS pathological process. Until now, few studies investigated the association between imbalance of CSF elements and severity of WNV infection. The aim of the present study was to apply the iTRAQ technology in order to identify the CSF proteins whose abundances are modified in patients with WNND. Forty-seven proteins were found modified in the CSF of WNND patients as compared to control groups, and most of them are reported for the first time in the context of WNND. On the basis of their known biological functions, several of these proteins were associated with inflammatory response. Among them, Defensin-1 alpha (DEFA1), a protein reported with anti-viral effects, presented the highest increasing fold-change (FC>12). The augmentation of DEFA1 abundance in patients with WNND was confirmed at the CSF, but also in serum, compared to the control individual groups. Furthermore, the DEFA1 serum level was significantly elevated in WNND patients compared to subjects diagnosed for WNF. The present study provided the first insight into the potential CSF biomarkers associated with WNV neuroinvasion. Further investigation in larger cohorts with kinetic sampling could determine the usefulness of measuring DEFA1 as diagnostic or prognostic biomarker of detrimental WNND evolution. |
format | Online Article Text |
id | pubmed-3973578 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-39735782014-04-04 Cerebrospinal Fluid Biomarker Candidates Associated with Human WNV Neuroinvasive Disease Fraisier, Christophe Papa, Anna Granjeaud, Samuel Hintzen, Rogier Martina, Byron Camoin, Luc Almeras, Lionel PLoS One Research Article During the last decade, the epidemiology of WNV in humans has changed in the southern regions of Europe, with high incidence of West Nile fever (WNF) cases, but also of West Nile neuroinvasive disease (WNND). The lack of human vaccine or specific treatment against WNV infection imparts a pressing need to characterize indicators associated with neurological involvement. By its intimacy with central nervous system (CNS) structures, modifications in the cerebrospinal fluid (CSF) composition could accurately reflect CNS pathological process. Until now, few studies investigated the association between imbalance of CSF elements and severity of WNV infection. The aim of the present study was to apply the iTRAQ technology in order to identify the CSF proteins whose abundances are modified in patients with WNND. Forty-seven proteins were found modified in the CSF of WNND patients as compared to control groups, and most of them are reported for the first time in the context of WNND. On the basis of their known biological functions, several of these proteins were associated with inflammatory response. Among them, Defensin-1 alpha (DEFA1), a protein reported with anti-viral effects, presented the highest increasing fold-change (FC>12). The augmentation of DEFA1 abundance in patients with WNND was confirmed at the CSF, but also in serum, compared to the control individual groups. Furthermore, the DEFA1 serum level was significantly elevated in WNND patients compared to subjects diagnosed for WNF. The present study provided the first insight into the potential CSF biomarkers associated with WNV neuroinvasion. Further investigation in larger cohorts with kinetic sampling could determine the usefulness of measuring DEFA1 as diagnostic or prognostic biomarker of detrimental WNND evolution. Public Library of Science 2014-04-02 /pmc/articles/PMC3973578/ /pubmed/24695528 http://dx.doi.org/10.1371/journal.pone.0093637 Text en © 2014 Fraisier et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Fraisier, Christophe Papa, Anna Granjeaud, Samuel Hintzen, Rogier Martina, Byron Camoin, Luc Almeras, Lionel Cerebrospinal Fluid Biomarker Candidates Associated with Human WNV Neuroinvasive Disease |
title | Cerebrospinal Fluid Biomarker Candidates Associated with Human WNV Neuroinvasive Disease |
title_full | Cerebrospinal Fluid Biomarker Candidates Associated with Human WNV Neuroinvasive Disease |
title_fullStr | Cerebrospinal Fluid Biomarker Candidates Associated with Human WNV Neuroinvasive Disease |
title_full_unstemmed | Cerebrospinal Fluid Biomarker Candidates Associated with Human WNV Neuroinvasive Disease |
title_short | Cerebrospinal Fluid Biomarker Candidates Associated with Human WNV Neuroinvasive Disease |
title_sort | cerebrospinal fluid biomarker candidates associated with human wnv neuroinvasive disease |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3973578/ https://www.ncbi.nlm.nih.gov/pubmed/24695528 http://dx.doi.org/10.1371/journal.pone.0093637 |
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