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Full Genome Virus Detection in Fecal Samples Using Sensitive Nucleic Acid Preparation, Deep Sequencing, and a Novel Iterative Sequence Classification Algorithm
We have developed a full genome virus detection process that combines sensitive nucleic acid preparation optimised for virus identification in fecal material with Illumina MiSeq sequencing and a novel post-sequencing virus identification algorithm. Enriched viral nucleic acid was converted to double...
Autores principales: | Cotten, Matthew, Oude Munnink, Bas, Canuti, Marta, Deijs, Martin, Watson, Simon J., Kellam, Paul, van der Hoek, Lia |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3973683/ https://www.ncbi.nlm.nih.gov/pubmed/24695106 http://dx.doi.org/10.1371/journal.pone.0093269 |
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