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Cell proliferation and neuroblast differentiation in the dentate gyrus of high-fat diet-fed mice are increased after rosiglitazone treatment

In this study, we determined how rosiglitazone (RSG) differentially affected hippocampal neurogenesis in mice fed a low-fat diet (LFD) or high-fat diet (HFD; 60% fat). LFD and HFD were given to the mice for 8 weeks. Four weeks after initiating the LFD and HFD feeding, vehicle or RSG was administered...

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Autores principales: Yoo, Dae Young, Kim, Woosuk, Kim, Dae Won, Nam, Sung Min, Jung, Hyo Young, Kim, Jong Whi, Lee, Choong Hyun, Choi, Jung Hoon, Won, Moo-Ho, Yoon, Yeo Sung, Hwang, In Koo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Veterinary Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3973763/
https://www.ncbi.nlm.nih.gov/pubmed/24136217
http://dx.doi.org/10.4142/jvs.2014.15.1.27
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author Yoo, Dae Young
Kim, Woosuk
Kim, Dae Won
Nam, Sung Min
Jung, Hyo Young
Kim, Jong Whi
Lee, Choong Hyun
Choi, Jung Hoon
Won, Moo-Ho
Yoon, Yeo Sung
Hwang, In Koo
author_facet Yoo, Dae Young
Kim, Woosuk
Kim, Dae Won
Nam, Sung Min
Jung, Hyo Young
Kim, Jong Whi
Lee, Choong Hyun
Choi, Jung Hoon
Won, Moo-Ho
Yoon, Yeo Sung
Hwang, In Koo
author_sort Yoo, Dae Young
collection PubMed
description In this study, we determined how rosiglitazone (RSG) differentially affected hippocampal neurogenesis in mice fed a low-fat diet (LFD) or high-fat diet (HFD; 60% fat). LFD and HFD were given to the mice for 8 weeks. Four weeks after initiating the LFD and HFD feeding, vehicle or RSG was administered orally once a day to both groups of mice. We measured cell proliferation and neuroblast differentiation in the subgranular zone of the dentate gyrus using Ki67 and doublecortin (DCX), respectively, as markers. In addition, we monitored the effects of RSG on the levels of DCX and brain-derived neurotrophic factor (BDNF) in hippocampal homogenates. At 8 weeks after the LFD feeding, the numbers of Ki67- and DCX-positive cells as well as hippocampal levels of DCX and BDNF were significantly decreased in the RSG-treated group compared to the vehicle-treated animals. In contrast, the numbers of Ki67- and DCX-positive cells along with hippocampal levels of DCX and BDNF in the HFD fed mice were significantly increased in the RSG-treated mice compared to the vehicle-treated group. Our data demonstrate that RSG can modulate the levels of BDNF, which could play a pivotal role in cell proliferation and neuroblast differentiation in the hippocampal dentate gyrus.
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spelling pubmed-39737632014-04-04 Cell proliferation and neuroblast differentiation in the dentate gyrus of high-fat diet-fed mice are increased after rosiglitazone treatment Yoo, Dae Young Kim, Woosuk Kim, Dae Won Nam, Sung Min Jung, Hyo Young Kim, Jong Whi Lee, Choong Hyun Choi, Jung Hoon Won, Moo-Ho Yoon, Yeo Sung Hwang, In Koo J Vet Sci Original Article In this study, we determined how rosiglitazone (RSG) differentially affected hippocampal neurogenesis in mice fed a low-fat diet (LFD) or high-fat diet (HFD; 60% fat). LFD and HFD were given to the mice for 8 weeks. Four weeks after initiating the LFD and HFD feeding, vehicle or RSG was administered orally once a day to both groups of mice. We measured cell proliferation and neuroblast differentiation in the subgranular zone of the dentate gyrus using Ki67 and doublecortin (DCX), respectively, as markers. In addition, we monitored the effects of RSG on the levels of DCX and brain-derived neurotrophic factor (BDNF) in hippocampal homogenates. At 8 weeks after the LFD feeding, the numbers of Ki67- and DCX-positive cells as well as hippocampal levels of DCX and BDNF were significantly decreased in the RSG-treated group compared to the vehicle-treated animals. In contrast, the numbers of Ki67- and DCX-positive cells along with hippocampal levels of DCX and BDNF in the HFD fed mice were significantly increased in the RSG-treated mice compared to the vehicle-treated group. Our data demonstrate that RSG can modulate the levels of BDNF, which could play a pivotal role in cell proliferation and neuroblast differentiation in the hippocampal dentate gyrus. The Korean Society of Veterinary Science 2014-03 2014-03-19 /pmc/articles/PMC3973763/ /pubmed/24136217 http://dx.doi.org/10.4142/jvs.2014.15.1.27 Text en © 2014 The Korean Society of Veterinary Science. http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Yoo, Dae Young
Kim, Woosuk
Kim, Dae Won
Nam, Sung Min
Jung, Hyo Young
Kim, Jong Whi
Lee, Choong Hyun
Choi, Jung Hoon
Won, Moo-Ho
Yoon, Yeo Sung
Hwang, In Koo
Cell proliferation and neuroblast differentiation in the dentate gyrus of high-fat diet-fed mice are increased after rosiglitazone treatment
title Cell proliferation and neuroblast differentiation in the dentate gyrus of high-fat diet-fed mice are increased after rosiglitazone treatment
title_full Cell proliferation and neuroblast differentiation in the dentate gyrus of high-fat diet-fed mice are increased after rosiglitazone treatment
title_fullStr Cell proliferation and neuroblast differentiation in the dentate gyrus of high-fat diet-fed mice are increased after rosiglitazone treatment
title_full_unstemmed Cell proliferation and neuroblast differentiation in the dentate gyrus of high-fat diet-fed mice are increased after rosiglitazone treatment
title_short Cell proliferation and neuroblast differentiation in the dentate gyrus of high-fat diet-fed mice are increased after rosiglitazone treatment
title_sort cell proliferation and neuroblast differentiation in the dentate gyrus of high-fat diet-fed mice are increased after rosiglitazone treatment
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3973763/
https://www.ncbi.nlm.nih.gov/pubmed/24136217
http://dx.doi.org/10.4142/jvs.2014.15.1.27
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