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Immature articular cartilage and subchondral bone covered by menisci are potentially susceptive to mechanical load

BACKGROUND: The differences of mechanical and histological properties between cartilage covered by menisci and uncovered by menisci may contribute to the osteoarthritis after meniscectomy and these differences are not fully understood. The purpose of this study is to investigate potential difference...

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Autores principales: Iijima, Hirotaka, Aoyama, Tomoki, Ito, Akira, Tajino, Junichi, Nagai, Momoko, Zhang, Xiangkai, Yamaguchi, Shoki, Akiyama, Haruhiko, Kuroki, Hiroshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3973825/
https://www.ncbi.nlm.nih.gov/pubmed/24669849
http://dx.doi.org/10.1186/1471-2474-15-101
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author Iijima, Hirotaka
Aoyama, Tomoki
Ito, Akira
Tajino, Junichi
Nagai, Momoko
Zhang, Xiangkai
Yamaguchi, Shoki
Akiyama, Haruhiko
Kuroki, Hiroshi
author_facet Iijima, Hirotaka
Aoyama, Tomoki
Ito, Akira
Tajino, Junichi
Nagai, Momoko
Zhang, Xiangkai
Yamaguchi, Shoki
Akiyama, Haruhiko
Kuroki, Hiroshi
author_sort Iijima, Hirotaka
collection PubMed
description BACKGROUND: The differences of mechanical and histological properties between cartilage covered by menisci and uncovered by menisci may contribute to the osteoarthritis after meniscectomy and these differences are not fully understood. The purpose of this study is to investigate potential differences in the mechanical and histological properties, and in particular the collagen architecture, of the superficial cartilage layer and subchondral bone between regions covered and uncovered by menisci using immature knee. METHODS: Osteochondral plugs were obtained from porcine tibial cartilage that was either covered or uncovered by menisci. Investigation of the thickness, mechanical properties, histology, and water content of the cartilage as well as micro-computed tomography analysis of the subchondral bone was performed to compare these regions. Collagen architecture was also assessed by using scanning electron microscopy. RESULTS: Compared to the cartilage uncovered by menisci, that covered by menisci was thinner and showed a higher deformity to compression loading and higher water content. In the superficial layer of cartilage in the uncovered regions, collagen fibers showed high density, whereas they showed low density in covered regions. Furthermore, subchondral bone architecture varied between the 2 regions, and showed low bone density in covered regions. CONCLUSIONS: Cartilage covered by menisci differed from that uncovered in both its mechanical and histological properties, especially with regards to the density of the superficial collagen layer. These regional differences may be related to local mechanical environment in normal condition and indicate that cartilage covered by menisci is tightly guarded by menisci from extreme mechanical loading. Our results indicate that immature cartilage degeneration and subchondral microfracture may occur easily to extreme direct mechanical loading in covered region after meniscectomy.
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spelling pubmed-39738252014-04-04 Immature articular cartilage and subchondral bone covered by menisci are potentially susceptive to mechanical load Iijima, Hirotaka Aoyama, Tomoki Ito, Akira Tajino, Junichi Nagai, Momoko Zhang, Xiangkai Yamaguchi, Shoki Akiyama, Haruhiko Kuroki, Hiroshi BMC Musculoskelet Disord Research Article BACKGROUND: The differences of mechanical and histological properties between cartilage covered by menisci and uncovered by menisci may contribute to the osteoarthritis after meniscectomy and these differences are not fully understood. The purpose of this study is to investigate potential differences in the mechanical and histological properties, and in particular the collagen architecture, of the superficial cartilage layer and subchondral bone between regions covered and uncovered by menisci using immature knee. METHODS: Osteochondral plugs were obtained from porcine tibial cartilage that was either covered or uncovered by menisci. Investigation of the thickness, mechanical properties, histology, and water content of the cartilage as well as micro-computed tomography analysis of the subchondral bone was performed to compare these regions. Collagen architecture was also assessed by using scanning electron microscopy. RESULTS: Compared to the cartilage uncovered by menisci, that covered by menisci was thinner and showed a higher deformity to compression loading and higher water content. In the superficial layer of cartilage in the uncovered regions, collagen fibers showed high density, whereas they showed low density in covered regions. Furthermore, subchondral bone architecture varied between the 2 regions, and showed low bone density in covered regions. CONCLUSIONS: Cartilage covered by menisci differed from that uncovered in both its mechanical and histological properties, especially with regards to the density of the superficial collagen layer. These regional differences may be related to local mechanical environment in normal condition and indicate that cartilage covered by menisci is tightly guarded by menisci from extreme mechanical loading. Our results indicate that immature cartilage degeneration and subchondral microfracture may occur easily to extreme direct mechanical loading in covered region after meniscectomy. BioMed Central 2014-03-26 /pmc/articles/PMC3973825/ /pubmed/24669849 http://dx.doi.org/10.1186/1471-2474-15-101 Text en Copyright © 2014 Iijima et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.
spellingShingle Research Article
Iijima, Hirotaka
Aoyama, Tomoki
Ito, Akira
Tajino, Junichi
Nagai, Momoko
Zhang, Xiangkai
Yamaguchi, Shoki
Akiyama, Haruhiko
Kuroki, Hiroshi
Immature articular cartilage and subchondral bone covered by menisci are potentially susceptive to mechanical load
title Immature articular cartilage and subchondral bone covered by menisci are potentially susceptive to mechanical load
title_full Immature articular cartilage and subchondral bone covered by menisci are potentially susceptive to mechanical load
title_fullStr Immature articular cartilage and subchondral bone covered by menisci are potentially susceptive to mechanical load
title_full_unstemmed Immature articular cartilage and subchondral bone covered by menisci are potentially susceptive to mechanical load
title_short Immature articular cartilage and subchondral bone covered by menisci are potentially susceptive to mechanical load
title_sort immature articular cartilage and subchondral bone covered by menisci are potentially susceptive to mechanical load
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3973825/
https://www.ncbi.nlm.nih.gov/pubmed/24669849
http://dx.doi.org/10.1186/1471-2474-15-101
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