Cargando…

Alterations in Both the Activatory and Inhibitory Potential of Peripheral Blood CD4+ T Cells in Rheumatoid Arthritis Patients Correlate with Disease Progression

The chronic nature of rheumatoid arthritis (RA) suggests immune dysfunction, including persistent systemic activation. Therefore, we evaluated the activatory and inhibitory potential as well as proliferative activity of peripheral blood (PB) CD4+ T cells from RA patients in different stages of the d...

Descripción completa

Detalles Bibliográficos
Autores principales: Kosmaczewska, Agata, Ciszak, Lidia, Swierkot, Jerzy, Szteblich, Aleksandra, Wiland, Piotr, Frydecka, Irena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3973952/
https://www.ncbi.nlm.nih.gov/pubmed/24019115
http://dx.doi.org/10.1007/s12253-013-9687-0
_version_ 1782479399703019520
author Kosmaczewska, Agata
Ciszak, Lidia
Swierkot, Jerzy
Szteblich, Aleksandra
Wiland, Piotr
Frydecka, Irena
author_facet Kosmaczewska, Agata
Ciszak, Lidia
Swierkot, Jerzy
Szteblich, Aleksandra
Wiland, Piotr
Frydecka, Irena
author_sort Kosmaczewska, Agata
collection PubMed
description The chronic nature of rheumatoid arthritis (RA) suggests immune dysfunction, including persistent systemic activation. Therefore, we evaluated the activatory and inhibitory potential as well as proliferative activity of peripheral blood (PB) CD4+ T cells from RA patients in different stages of the disease and after different therapeutic interventions. We found that CD4+ T cells from RA patients were activated in vivo concerning decreased CD28 expression and increase of CD40L, CD69, and CTLA-4 expression; however, the extent of stimulation was suboptimal when compared to healthy controls. Consequently, impaired proliferative activities of these cells were found in all patients irrespective of the active disease duration. Treatment with methotrexate (MTX) and/or inhibitors of TNF-alpha (iTNF) did not significantly influence systemic activation in RA patients, which corresponded with the maintenance of inflammation markers; however, partial restoration of CD28 and CTLA-4 expression as well as clinical improvement were observed. In patients with early disease (the MTX group), we noted higher capacity of CD4+ T cells for restoration of T cell function, whereas cells from the iTNF group with progressive disease remained with a proliferative defect after the treatment. In conclusion, our study demonstrates that the dysregulated expression of molecules interfering with CD4+ T cell signaling may result in functional impairment of the effector T cells and correlates with disease progression.
format Online
Article
Text
id pubmed-3973952
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Springer Netherlands
record_format MEDLINE/PubMed
spelling pubmed-39739522014-04-07 Alterations in Both the Activatory and Inhibitory Potential of Peripheral Blood CD4+ T Cells in Rheumatoid Arthritis Patients Correlate with Disease Progression Kosmaczewska, Agata Ciszak, Lidia Swierkot, Jerzy Szteblich, Aleksandra Wiland, Piotr Frydecka, Irena Pathol Oncol Res Research The chronic nature of rheumatoid arthritis (RA) suggests immune dysfunction, including persistent systemic activation. Therefore, we evaluated the activatory and inhibitory potential as well as proliferative activity of peripheral blood (PB) CD4+ T cells from RA patients in different stages of the disease and after different therapeutic interventions. We found that CD4+ T cells from RA patients were activated in vivo concerning decreased CD28 expression and increase of CD40L, CD69, and CTLA-4 expression; however, the extent of stimulation was suboptimal when compared to healthy controls. Consequently, impaired proliferative activities of these cells were found in all patients irrespective of the active disease duration. Treatment with methotrexate (MTX) and/or inhibitors of TNF-alpha (iTNF) did not significantly influence systemic activation in RA patients, which corresponded with the maintenance of inflammation markers; however, partial restoration of CD28 and CTLA-4 expression as well as clinical improvement were observed. In patients with early disease (the MTX group), we noted higher capacity of CD4+ T cells for restoration of T cell function, whereas cells from the iTNF group with progressive disease remained with a proliferative defect after the treatment. In conclusion, our study demonstrates that the dysregulated expression of molecules interfering with CD4+ T cell signaling may result in functional impairment of the effector T cells and correlates with disease progression. Springer Netherlands 2013-09-10 2014 /pmc/articles/PMC3973952/ /pubmed/24019115 http://dx.doi.org/10.1007/s12253-013-9687-0 Text en © The Author(s) 2013 https://creativecommons.org/licenses/by-nc/2.0/ Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Research
Kosmaczewska, Agata
Ciszak, Lidia
Swierkot, Jerzy
Szteblich, Aleksandra
Wiland, Piotr
Frydecka, Irena
Alterations in Both the Activatory and Inhibitory Potential of Peripheral Blood CD4+ T Cells in Rheumatoid Arthritis Patients Correlate with Disease Progression
title Alterations in Both the Activatory and Inhibitory Potential of Peripheral Blood CD4+ T Cells in Rheumatoid Arthritis Patients Correlate with Disease Progression
title_full Alterations in Both the Activatory and Inhibitory Potential of Peripheral Blood CD4+ T Cells in Rheumatoid Arthritis Patients Correlate with Disease Progression
title_fullStr Alterations in Both the Activatory and Inhibitory Potential of Peripheral Blood CD4+ T Cells in Rheumatoid Arthritis Patients Correlate with Disease Progression
title_full_unstemmed Alterations in Both the Activatory and Inhibitory Potential of Peripheral Blood CD4+ T Cells in Rheumatoid Arthritis Patients Correlate with Disease Progression
title_short Alterations in Both the Activatory and Inhibitory Potential of Peripheral Blood CD4+ T Cells in Rheumatoid Arthritis Patients Correlate with Disease Progression
title_sort alterations in both the activatory and inhibitory potential of peripheral blood cd4+ t cells in rheumatoid arthritis patients correlate with disease progression
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3973952/
https://www.ncbi.nlm.nih.gov/pubmed/24019115
http://dx.doi.org/10.1007/s12253-013-9687-0
work_keys_str_mv AT kosmaczewskaagata alterationsinboththeactivatoryandinhibitorypotentialofperipheralbloodcd4tcellsinrheumatoidarthritispatientscorrelatewithdiseaseprogression
AT ciszaklidia alterationsinboththeactivatoryandinhibitorypotentialofperipheralbloodcd4tcellsinrheumatoidarthritispatientscorrelatewithdiseaseprogression
AT swierkotjerzy alterationsinboththeactivatoryandinhibitorypotentialofperipheralbloodcd4tcellsinrheumatoidarthritispatientscorrelatewithdiseaseprogression
AT szteblichaleksandra alterationsinboththeactivatoryandinhibitorypotentialofperipheralbloodcd4tcellsinrheumatoidarthritispatientscorrelatewithdiseaseprogression
AT wilandpiotr alterationsinboththeactivatoryandinhibitorypotentialofperipheralbloodcd4tcellsinrheumatoidarthritispatientscorrelatewithdiseaseprogression
AT frydeckairena alterationsinboththeactivatoryandinhibitorypotentialofperipheralbloodcd4tcellsinrheumatoidarthritispatientscorrelatewithdiseaseprogression