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Impact of HIV co-infection on plasma level of cytokines and chemokines of pulmonary tuberculosis patients

BACKGROUND: The immunologic environment during HIV/M. tuberculosis co-infection is characterized by cytokine and chemokine irregularities that have been shown to increase immune activation, viral replication, and T cell dysfunction. METHODS: We analysed ex vivo plasma samples from 17 HIV negative an...

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Autores principales: Mihret, Adane, Abebe, Markos, Bekele, Yonas, Aseffa, Abraham, Walzl, Gerhard, Howe, Rawleigh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3974017/
https://www.ncbi.nlm.nih.gov/pubmed/24592945
http://dx.doi.org/10.1186/1471-2334-14-125
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author Mihret, Adane
Abebe, Markos
Bekele, Yonas
Aseffa, Abraham
Walzl, Gerhard
Howe, Rawleigh
author_facet Mihret, Adane
Abebe, Markos
Bekele, Yonas
Aseffa, Abraham
Walzl, Gerhard
Howe, Rawleigh
author_sort Mihret, Adane
collection PubMed
description BACKGROUND: The immunologic environment during HIV/M. tuberculosis co-infection is characterized by cytokine and chemokine irregularities that have been shown to increase immune activation, viral replication, and T cell dysfunction. METHODS: We analysed ex vivo plasma samples from 17 HIV negative and 16 HIV pulmonary tuberculosis co infected cases using Luminex assay to see impact of HIV co-infection on plasma level of cytokines and chemokines of pulmonary tuberculosis patients before and after anti Tuberculosis treatment. RESULTS: The median plasma level of IFN-γ, IL-4, MCP-3, MIP-1β and IP-10 was significantly different (P < 0.05) before and after treatment in HIV negative TB patients but not in HIV positive TB patients. There was no significant difference between HIV positive and HIV negative TB patients (P > 0.05) in the plasma level of any of the cytokines or chemokines before treatment and anti TB treatment did not change the level of any of the measured cytokines in HIV positive tuberculosis patients. The ratio of IFN-γ/IL-10 and IFN-γ/IL-4 showed a significant increase after treatment in HIV negative TB cases but not in HIV positive TB cases which might indicate prolonged impairment of immune response to TB in HIV positive TB patients as compared to HIV negative tuberculosis patients. CONCLUSIONS: HIV positive and HIV negative Tuberculosis patients display similar plasma cytokine and chemokine pattern. However, anti TB treatment significantly improves the Th1 cytokines and level of chemokines but does not restore the immune response in HIV positive individuals.
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spelling pubmed-39740172014-04-04 Impact of HIV co-infection on plasma level of cytokines and chemokines of pulmonary tuberculosis patients Mihret, Adane Abebe, Markos Bekele, Yonas Aseffa, Abraham Walzl, Gerhard Howe, Rawleigh BMC Infect Dis Research Article BACKGROUND: The immunologic environment during HIV/M. tuberculosis co-infection is characterized by cytokine and chemokine irregularities that have been shown to increase immune activation, viral replication, and T cell dysfunction. METHODS: We analysed ex vivo plasma samples from 17 HIV negative and 16 HIV pulmonary tuberculosis co infected cases using Luminex assay to see impact of HIV co-infection on plasma level of cytokines and chemokines of pulmonary tuberculosis patients before and after anti Tuberculosis treatment. RESULTS: The median plasma level of IFN-γ, IL-4, MCP-3, MIP-1β and IP-10 was significantly different (P < 0.05) before and after treatment in HIV negative TB patients but not in HIV positive TB patients. There was no significant difference between HIV positive and HIV negative TB patients (P > 0.05) in the plasma level of any of the cytokines or chemokines before treatment and anti TB treatment did not change the level of any of the measured cytokines in HIV positive tuberculosis patients. The ratio of IFN-γ/IL-10 and IFN-γ/IL-4 showed a significant increase after treatment in HIV negative TB cases but not in HIV positive TB cases which might indicate prolonged impairment of immune response to TB in HIV positive TB patients as compared to HIV negative tuberculosis patients. CONCLUSIONS: HIV positive and HIV negative Tuberculosis patients display similar plasma cytokine and chemokine pattern. However, anti TB treatment significantly improves the Th1 cytokines and level of chemokines but does not restore the immune response in HIV positive individuals. BioMed Central 2014-03-04 /pmc/articles/PMC3974017/ /pubmed/24592945 http://dx.doi.org/10.1186/1471-2334-14-125 Text en Copyright © 2014 Mihret et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Mihret, Adane
Abebe, Markos
Bekele, Yonas
Aseffa, Abraham
Walzl, Gerhard
Howe, Rawleigh
Impact of HIV co-infection on plasma level of cytokines and chemokines of pulmonary tuberculosis patients
title Impact of HIV co-infection on plasma level of cytokines and chemokines of pulmonary tuberculosis patients
title_full Impact of HIV co-infection on plasma level of cytokines and chemokines of pulmonary tuberculosis patients
title_fullStr Impact of HIV co-infection on plasma level of cytokines and chemokines of pulmonary tuberculosis patients
title_full_unstemmed Impact of HIV co-infection on plasma level of cytokines and chemokines of pulmonary tuberculosis patients
title_short Impact of HIV co-infection on plasma level of cytokines and chemokines of pulmonary tuberculosis patients
title_sort impact of hiv co-infection on plasma level of cytokines and chemokines of pulmonary tuberculosis patients
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3974017/
https://www.ncbi.nlm.nih.gov/pubmed/24592945
http://dx.doi.org/10.1186/1471-2334-14-125
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