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EIF5A2 predicts outcome in localised invasive bladder cancer and promotes bladder cancer cell aggressiveness in vitro and in vivo

BACKGROUND: EIF5A2, eukaryotic translation initiation factor 5A2, is associated with several human cancers. In this study, we investigated the role of EIF5A2 in the metastatic potential of localised invasive bladder cancer (BC) and its underlying molecular mechanisms were explored. METHODS: The expr...

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Autores principales: Wei, J-H, Cao, J-Z, Zhang, D, Liao, B, Zhong, W-M, Lu, J, Zhao, H-W, Zhang, J-X, Tong, Z-T, Fan, S, Liang, C-Z, Liao, Y-B, Pang, J, Wu, R-H, Fang, Y, Chen, Z-H, Li, B, Xie, D, Chen, W, Luo, J-H
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3974079/
https://www.ncbi.nlm.nih.gov/pubmed/24504366
http://dx.doi.org/10.1038/bjc.2014.52
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author Wei, J-H
Cao, J-Z
Zhang, D
Liao, B
Zhong, W-M
Lu, J
Zhao, H-W
Zhang, J-X
Tong, Z-T
Fan, S
Liang, C-Z
Liao, Y-B
Pang, J
Wu, R-H
Fang, Y
Chen, Z-H
Li, B
Xie, D
Chen, W
Luo, J-H
author_facet Wei, J-H
Cao, J-Z
Zhang, D
Liao, B
Zhong, W-M
Lu, J
Zhao, H-W
Zhang, J-X
Tong, Z-T
Fan, S
Liang, C-Z
Liao, Y-B
Pang, J
Wu, R-H
Fang, Y
Chen, Z-H
Li, B
Xie, D
Chen, W
Luo, J-H
author_sort Wei, J-H
collection PubMed
description BACKGROUND: EIF5A2, eukaryotic translation initiation factor 5A2, is associated with several human cancers. In this study, we investigated the role of EIF5A2 in the metastatic potential of localised invasive bladder cancer (BC) and its underlying molecular mechanisms were explored. METHODS: The expression pattern of EIF5A2 in localised invasive BC was determined by immunohistochemistry. In addition, the function of EIF5A2 in BC and its underlying mechanisms were elucidated with a series of in vitro and in vivo assays. RESULTS: Overexpression of EIF5A2 was an independent predictor for poor metastasis-free survival of localised invasive BC patients treated with radical cystectomy. Knockdown of EIF5A2 inhibited BC cell migratory and invasive capacities in vitro and metastatic potential in vivo and reversed epithelial–mesenchymal transition (EMT), whereas overexpression of EIF5A2 promoted BC cells motility and invasiveness in vitro and metastatic potential in vivo and induced EMT. In addition, we found that EIF5A2 might activate TGF-β1 expression to induce EMT and drive aggressiveness in BC cells. EIF5A2 stabilized STAT3 and stimulated nuclear localisation of STAT3, which resulted in increasing enrichment of STAT3 onto TGF-β1 promoter to enhance the transcription of TGF-β1. CONCLUSIONS: EIF5A2 overexpression predicts tumour metastatic potential in patients with localised invasive BC treated with radical cystectomy. Furthermore, EIF5A2 elevated TGF-β1 expression through STAT3 to induce EMT and promotes aggressiveness in BC.
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spelling pubmed-39740792015-04-01 EIF5A2 predicts outcome in localised invasive bladder cancer and promotes bladder cancer cell aggressiveness in vitro and in vivo Wei, J-H Cao, J-Z Zhang, D Liao, B Zhong, W-M Lu, J Zhao, H-W Zhang, J-X Tong, Z-T Fan, S Liang, C-Z Liao, Y-B Pang, J Wu, R-H Fang, Y Chen, Z-H Li, B Xie, D Chen, W Luo, J-H Br J Cancer Molecular Diagnostics BACKGROUND: EIF5A2, eukaryotic translation initiation factor 5A2, is associated with several human cancers. In this study, we investigated the role of EIF5A2 in the metastatic potential of localised invasive bladder cancer (BC) and its underlying molecular mechanisms were explored. METHODS: The expression pattern of EIF5A2 in localised invasive BC was determined by immunohistochemistry. In addition, the function of EIF5A2 in BC and its underlying mechanisms were elucidated with a series of in vitro and in vivo assays. RESULTS: Overexpression of EIF5A2 was an independent predictor for poor metastasis-free survival of localised invasive BC patients treated with radical cystectomy. Knockdown of EIF5A2 inhibited BC cell migratory and invasive capacities in vitro and metastatic potential in vivo and reversed epithelial–mesenchymal transition (EMT), whereas overexpression of EIF5A2 promoted BC cells motility and invasiveness in vitro and metastatic potential in vivo and induced EMT. In addition, we found that EIF5A2 might activate TGF-β1 expression to induce EMT and drive aggressiveness in BC cells. EIF5A2 stabilized STAT3 and stimulated nuclear localisation of STAT3, which resulted in increasing enrichment of STAT3 onto TGF-β1 promoter to enhance the transcription of TGF-β1. CONCLUSIONS: EIF5A2 overexpression predicts tumour metastatic potential in patients with localised invasive BC treated with radical cystectomy. Furthermore, EIF5A2 elevated TGF-β1 expression through STAT3 to induce EMT and promotes aggressiveness in BC. Nature Publishing Group 2014-04-01 2014-02-06 /pmc/articles/PMC3974079/ /pubmed/24504366 http://dx.doi.org/10.1038/bjc.2014.52 Text en Copyright © 2014 Cancer Research UK http://creativecommons.org/licenses/by-nc-sa/3.0/ From twelve months after its original publication, this work is licensed under the Creative Commons Attribution-NonCommercial-Share Alike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/
spellingShingle Molecular Diagnostics
Wei, J-H
Cao, J-Z
Zhang, D
Liao, B
Zhong, W-M
Lu, J
Zhao, H-W
Zhang, J-X
Tong, Z-T
Fan, S
Liang, C-Z
Liao, Y-B
Pang, J
Wu, R-H
Fang, Y
Chen, Z-H
Li, B
Xie, D
Chen, W
Luo, J-H
EIF5A2 predicts outcome in localised invasive bladder cancer and promotes bladder cancer cell aggressiveness in vitro and in vivo
title EIF5A2 predicts outcome in localised invasive bladder cancer and promotes bladder cancer cell aggressiveness in vitro and in vivo
title_full EIF5A2 predicts outcome in localised invasive bladder cancer and promotes bladder cancer cell aggressiveness in vitro and in vivo
title_fullStr EIF5A2 predicts outcome in localised invasive bladder cancer and promotes bladder cancer cell aggressiveness in vitro and in vivo
title_full_unstemmed EIF5A2 predicts outcome in localised invasive bladder cancer and promotes bladder cancer cell aggressiveness in vitro and in vivo
title_short EIF5A2 predicts outcome in localised invasive bladder cancer and promotes bladder cancer cell aggressiveness in vitro and in vivo
title_sort eif5a2 predicts outcome in localised invasive bladder cancer and promotes bladder cancer cell aggressiveness in vitro and in vivo
topic Molecular Diagnostics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3974079/
https://www.ncbi.nlm.nih.gov/pubmed/24504366
http://dx.doi.org/10.1038/bjc.2014.52
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