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Platelet extracts induce growth, migration and invasion in human hepatocellular carcinoma in vitro
BACKGROUND: Thrombocytopenia has been reported to be associated with small size HCCs, and thrombocytosis to be associated with large size HCCs. The aim was to examine the effects of platelets in relation to HCC cell growth. METHODS: The effects of time-expired pooled normal human platelets were exam...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3974148/ https://www.ncbi.nlm.nih.gov/pubmed/24468103 http://dx.doi.org/10.1186/1471-2407-14-43 |
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author | Carr, Brian I Cavallini, Aldo D’Alessandro, Rosalba Refolo, Maria Grazia Lippolis, Catia Mazzocca, Antonio Messa, Caterina |
author_facet | Carr, Brian I Cavallini, Aldo D’Alessandro, Rosalba Refolo, Maria Grazia Lippolis, Catia Mazzocca, Antonio Messa, Caterina |
author_sort | Carr, Brian I |
collection | PubMed |
description | BACKGROUND: Thrombocytopenia has been reported to be associated with small size HCCs, and thrombocytosis to be associated with large size HCCs. The aim was to examine the effects of platelets in relation to HCC cell growth. METHODS: The effects of time-expired pooled normal human platelets were examined on human HCC cell line growth and invasion. RESULTS: Blood platelet numbers increased with increasing HCC tumor size and portal vein invasion. Platelet extracts enhanced cell growth in 4 human HCC cell lines, as well as cell migration, medium AFP levels and decreased apoptosis. Cell invasion was significantly enhanced, using a Matrigel-coated trans-well membrane and3D (Real-Time Imaging) invasion assay. Western blots showed that platelets caused enhanced phospho-ERK and phospho–JNK signaling and anti-apoptotic effect with increase of Bcl-xL (anti-apoptotic marker) and decrease of Bid (pro-apoptotic marker) levels. Their growth effects were blocked by a JNK inhibitor. CONCLUSIONS: Platelets stimulated growth and invasion of several HCC cell lines in vitro, suggesting that platelets or platelet growth factors could be a potential pharmacological target. |
format | Online Article Text |
id | pubmed-3974148 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-39741482014-04-04 Platelet extracts induce growth, migration and invasion in human hepatocellular carcinoma in vitro Carr, Brian I Cavallini, Aldo D’Alessandro, Rosalba Refolo, Maria Grazia Lippolis, Catia Mazzocca, Antonio Messa, Caterina BMC Cancer Research Article BACKGROUND: Thrombocytopenia has been reported to be associated with small size HCCs, and thrombocytosis to be associated with large size HCCs. The aim was to examine the effects of platelets in relation to HCC cell growth. METHODS: The effects of time-expired pooled normal human platelets were examined on human HCC cell line growth and invasion. RESULTS: Blood platelet numbers increased with increasing HCC tumor size and portal vein invasion. Platelet extracts enhanced cell growth in 4 human HCC cell lines, as well as cell migration, medium AFP levels and decreased apoptosis. Cell invasion was significantly enhanced, using a Matrigel-coated trans-well membrane and3D (Real-Time Imaging) invasion assay. Western blots showed that platelets caused enhanced phospho-ERK and phospho–JNK signaling and anti-apoptotic effect with increase of Bcl-xL (anti-apoptotic marker) and decrease of Bid (pro-apoptotic marker) levels. Their growth effects were blocked by a JNK inhibitor. CONCLUSIONS: Platelets stimulated growth and invasion of several HCC cell lines in vitro, suggesting that platelets or platelet growth factors could be a potential pharmacological target. BioMed Central 2014-01-27 /pmc/articles/PMC3974148/ /pubmed/24468103 http://dx.doi.org/10.1186/1471-2407-14-43 Text en Copyright © 2014 Carr et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Carr, Brian I Cavallini, Aldo D’Alessandro, Rosalba Refolo, Maria Grazia Lippolis, Catia Mazzocca, Antonio Messa, Caterina Platelet extracts induce growth, migration and invasion in human hepatocellular carcinoma in vitro |
title | Platelet extracts induce growth, migration and invasion in human hepatocellular carcinoma in vitro |
title_full | Platelet extracts induce growth, migration and invasion in human hepatocellular carcinoma in vitro |
title_fullStr | Platelet extracts induce growth, migration and invasion in human hepatocellular carcinoma in vitro |
title_full_unstemmed | Platelet extracts induce growth, migration and invasion in human hepatocellular carcinoma in vitro |
title_short | Platelet extracts induce growth, migration and invasion in human hepatocellular carcinoma in vitro |
title_sort | platelet extracts induce growth, migration and invasion in human hepatocellular carcinoma in vitro |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3974148/ https://www.ncbi.nlm.nih.gov/pubmed/24468103 http://dx.doi.org/10.1186/1471-2407-14-43 |
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