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Extended genetic analysis of Brazilian isolates of Bacillus cereus and Bacillus thuringiensis

Multiple locus sequence typing (MLST) was undertaken to extend the genetic characterization of 29 isolates of Bacillus cereus and Bacillus thuringiensis previously characterized in terms of presence/absence of sequences encoding virulence factors and via variable number tandem repeat (VNTR). Additio...

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Autores principales: Zahner, Viviane, Silva, Ana Carolina Telles de Carvalho e, de Moraes, Gabriela Pinhel, McIntosh, Douglas, de Filippis, Ivano
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Instituto Oswaldo Cruz, Ministério da Saúde 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3974328/
https://www.ncbi.nlm.nih.gov/pubmed/23440117
http://dx.doi.org/10.1590/S0074-02762013000100011
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author Zahner, Viviane
Silva, Ana Carolina Telles de Carvalho e
de Moraes, Gabriela Pinhel
McIntosh, Douglas
de Filippis, Ivano
author_facet Zahner, Viviane
Silva, Ana Carolina Telles de Carvalho e
de Moraes, Gabriela Pinhel
McIntosh, Douglas
de Filippis, Ivano
author_sort Zahner, Viviane
collection PubMed
description Multiple locus sequence typing (MLST) was undertaken to extend the genetic characterization of 29 isolates of Bacillus cereus and Bacillus thuringiensis previously characterized in terms of presence/absence of sequences encoding virulence factors and via variable number tandem repeat (VNTR). Additional analysis involved polymerase chain reaction for the presence of sequences (be, cytK, inA, pag, lef, cya and cap), encoding putative virulence factors, not investigated in the earlier study. MLST analysis ascribed novel and unique sequence types to each of the isolates. A phylogenetic tree was constructed from a single sequence of 2,838 bp of concatenated loci sequences. The strains were not monophyletic by analysis of any specific housekeeping gene or virulence characteristic. No clear association in relation to source of isolation or to genotypic profile based on the presence or absence of putative virulence genes could be identified. Comparison of VNTR profiling with MLST data suggested a correlation between these two methods of genetic analysis. In common with the majority of previous studies, MLST was unable to provide clarification of the basis for pathogenicity among members of the B. cereus complex. Nevertheless, our application of MLST served to reinforce the notion that B. cereus and B. thuringiensis should be considered as the same species.
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spelling pubmed-39743282014-05-21 Extended genetic analysis of Brazilian isolates of Bacillus cereus and Bacillus thuringiensis Zahner, Viviane Silva, Ana Carolina Telles de Carvalho e de Moraes, Gabriela Pinhel McIntosh, Douglas de Filippis, Ivano Mem Inst Oswaldo Cruz Articles Multiple locus sequence typing (MLST) was undertaken to extend the genetic characterization of 29 isolates of Bacillus cereus and Bacillus thuringiensis previously characterized in terms of presence/absence of sequences encoding virulence factors and via variable number tandem repeat (VNTR). Additional analysis involved polymerase chain reaction for the presence of sequences (be, cytK, inA, pag, lef, cya and cap), encoding putative virulence factors, not investigated in the earlier study. MLST analysis ascribed novel and unique sequence types to each of the isolates. A phylogenetic tree was constructed from a single sequence of 2,838 bp of concatenated loci sequences. The strains were not monophyletic by analysis of any specific housekeeping gene or virulence characteristic. No clear association in relation to source of isolation or to genotypic profile based on the presence or absence of putative virulence genes could be identified. Comparison of VNTR profiling with MLST data suggested a correlation between these two methods of genetic analysis. In common with the majority of previous studies, MLST was unable to provide clarification of the basis for pathogenicity among members of the B. cereus complex. Nevertheless, our application of MLST served to reinforce the notion that B. cereus and B. thuringiensis should be considered as the same species. Instituto Oswaldo Cruz, Ministério da Saúde 2013-02 /pmc/articles/PMC3974328/ /pubmed/23440117 http://dx.doi.org/10.1590/S0074-02762013000100011 Text en http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Articles
Zahner, Viviane
Silva, Ana Carolina Telles de Carvalho e
de Moraes, Gabriela Pinhel
McIntosh, Douglas
de Filippis, Ivano
Extended genetic analysis of Brazilian isolates of Bacillus cereus and Bacillus thuringiensis
title Extended genetic analysis of Brazilian isolates of Bacillus cereus and Bacillus thuringiensis
title_full Extended genetic analysis of Brazilian isolates of Bacillus cereus and Bacillus thuringiensis
title_fullStr Extended genetic analysis of Brazilian isolates of Bacillus cereus and Bacillus thuringiensis
title_full_unstemmed Extended genetic analysis of Brazilian isolates of Bacillus cereus and Bacillus thuringiensis
title_short Extended genetic analysis of Brazilian isolates of Bacillus cereus and Bacillus thuringiensis
title_sort extended genetic analysis of brazilian isolates of bacillus cereus and bacillus thuringiensis
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3974328/
https://www.ncbi.nlm.nih.gov/pubmed/23440117
http://dx.doi.org/10.1590/S0074-02762013000100011
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