A survey strategy for human respiratory syncytial virus detection among haematopoietic stem cell transplant patients: epidemiological and methodological analysis

Human respiratory syncytial virus (HRSV) causes severe infections among children and immunocompromised patients. We compared HRSV infections among Haematopoietic Stem Cell Transplant program (HSCT) patients and children using direct immunofluorescence (DFA), point-of-care RSV Bio Easy((r)) and a pol...

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Autores principales: Moreira, Luciana Peniche, Watanabe, Aripuanã Sakurada Aranha, Carraro, Emerson, da Silva, Ellen Ricci Monteiro, Guatura, Sandra Baltazar, Granato, Celso, Bellei, Nancy Junqueira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Instituto Oswaldo Cruz, Ministério da Saúde 2013
Materias:
Short Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3974330/
https://www.ncbi.nlm.nih.gov/pubmed/23440127
http://dx.doi.org/10.1590/S0074-02762013000100021
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author Moreira, Luciana Peniche
Watanabe, Aripuanã Sakurada Aranha
Carraro, Emerson
da Silva, Ellen Ricci Monteiro
Guatura, Sandra Baltazar
Granato, Celso
Bellei, Nancy Junqueira
author_facet Moreira, Luciana Peniche
Watanabe, Aripuanã Sakurada Aranha
Carraro, Emerson
da Silva, Ellen Ricci Monteiro
Guatura, Sandra Baltazar
Granato, Celso
Bellei, Nancy Junqueira
author_sort Moreira, Luciana Peniche
collection PubMed
description Human respiratory syncytial virus (HRSV) causes severe infections among children and immunocompromised patients. We compared HRSV infections among Haematopoietic Stem Cell Transplant program (HSCT) patients and children using direct immunofluorescence (DFA), point-of-care RSV Bio Easy((r)) and a polymerase chain reaction (PCR) assay. Overall, 102 samples from HSCT patients and 128 from children obtained positivity rate of 18.6% and 14.1% respectively. PCR sensitivity was highest mainly on samples collected after five days of symptoms onset. A combination of both DFA and reverse transcriptase-PCR methods for HSCT high-risk patients is the best diagnostic flow for HRSV diagnosis among these patients.
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spelling pubmed-39743302014-05-21 A survey strategy for human respiratory syncytial virus detection among haematopoietic stem cell transplant patients: epidemiological and methodological analysis Moreira, Luciana Peniche Watanabe, Aripuanã Sakurada Aranha Carraro, Emerson da Silva, Ellen Ricci Monteiro Guatura, Sandra Baltazar Granato, Celso Bellei, Nancy Junqueira Mem Inst Oswaldo Cruz Short Communications Human respiratory syncytial virus (HRSV) causes severe infections among children and immunocompromised patients. We compared HRSV infections among Haematopoietic Stem Cell Transplant program (HSCT) patients and children using direct immunofluorescence (DFA), point-of-care RSV Bio Easy((r)) and a polymerase chain reaction (PCR) assay. Overall, 102 samples from HSCT patients and 128 from children obtained positivity rate of 18.6% and 14.1% respectively. PCR sensitivity was highest mainly on samples collected after five days of symptoms onset. A combination of both DFA and reverse transcriptase-PCR methods for HSCT high-risk patients is the best diagnostic flow for HRSV diagnosis among these patients. Instituto Oswaldo Cruz, Ministério da Saúde 2013-02 /pmc/articles/PMC3974330/ /pubmed/23440127 http://dx.doi.org/10.1590/S0074-02762013000100021 Text en http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communications
Moreira, Luciana Peniche
Watanabe, Aripuanã Sakurada Aranha
Carraro, Emerson
da Silva, Ellen Ricci Monteiro
Guatura, Sandra Baltazar
Granato, Celso
Bellei, Nancy Junqueira
A survey strategy for human respiratory syncytial virus detection among haematopoietic stem cell transplant patients: epidemiological and methodological analysis
title A survey strategy for human respiratory syncytial virus detection among haematopoietic stem cell transplant patients: epidemiological and methodological analysis
title_full A survey strategy for human respiratory syncytial virus detection among haematopoietic stem cell transplant patients: epidemiological and methodological analysis
title_fullStr A survey strategy for human respiratory syncytial virus detection among haematopoietic stem cell transplant patients: epidemiological and methodological analysis
title_full_unstemmed A survey strategy for human respiratory syncytial virus detection among haematopoietic stem cell transplant patients: epidemiological and methodological analysis
title_short A survey strategy for human respiratory syncytial virus detection among haematopoietic stem cell transplant patients: epidemiological and methodological analysis
title_sort survey strategy for human respiratory syncytial virus detection among haematopoietic stem cell transplant patients: epidemiological and methodological analysis
topic Short Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3974330/
https://www.ncbi.nlm.nih.gov/pubmed/23440127
http://dx.doi.org/10.1590/S0074-02762013000100021
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