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Autophagy in Trypanosoma brucei: Amino Acid Requirement and Regulation during Different Growth Phases

Autophagy in the protozoan parasite, Trypanosoma brucei, may be involved in differentiation between different life cycle forms and during growth in culture. We have generated multiple parasite cell lines stably expressing green fluorescent protein- or hemagglutinin-tagged forms of the autophagy mark...

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Autores principales: Schmidt, Remo S., Bütikofer, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3974859/
https://www.ncbi.nlm.nih.gov/pubmed/24699810
http://dx.doi.org/10.1371/journal.pone.0093875
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author Schmidt, Remo S.
Bütikofer, Peter
author_facet Schmidt, Remo S.
Bütikofer, Peter
author_sort Schmidt, Remo S.
collection PubMed
description Autophagy in the protozoan parasite, Trypanosoma brucei, may be involved in differentiation between different life cycle forms and during growth in culture. We have generated multiple parasite cell lines stably expressing green fluorescent protein- or hemagglutinin-tagged forms of the autophagy marker proteins, TbAtg8.1 and TbAtg8.2, in T. brucei procyclic forms to establish a trypanosome system for quick and reliable determination of autophagy under different culture conditions using flow cytometry. We found that starvation-induced autophagy in T. brucei can be inhibited by addition of a single amino acid, histidine, to the incubation buffer. In addition, we show that autophagy is induced when parasites enter stationary growth phase in culture and that their capacity to undergo starvation-induced autophagy decreases with increasing cell density.
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spelling pubmed-39748592014-04-08 Autophagy in Trypanosoma brucei: Amino Acid Requirement and Regulation during Different Growth Phases Schmidt, Remo S. Bütikofer, Peter PLoS One Research Article Autophagy in the protozoan parasite, Trypanosoma brucei, may be involved in differentiation between different life cycle forms and during growth in culture. We have generated multiple parasite cell lines stably expressing green fluorescent protein- or hemagglutinin-tagged forms of the autophagy marker proteins, TbAtg8.1 and TbAtg8.2, in T. brucei procyclic forms to establish a trypanosome system for quick and reliable determination of autophagy under different culture conditions using flow cytometry. We found that starvation-induced autophagy in T. brucei can be inhibited by addition of a single amino acid, histidine, to the incubation buffer. In addition, we show that autophagy is induced when parasites enter stationary growth phase in culture and that their capacity to undergo starvation-induced autophagy decreases with increasing cell density. Public Library of Science 2014-04-03 /pmc/articles/PMC3974859/ /pubmed/24699810 http://dx.doi.org/10.1371/journal.pone.0093875 Text en © 2014 Schmidt, Bütikofer http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Schmidt, Remo S.
Bütikofer, Peter
Autophagy in Trypanosoma brucei: Amino Acid Requirement and Regulation during Different Growth Phases
title Autophagy in Trypanosoma brucei: Amino Acid Requirement and Regulation during Different Growth Phases
title_full Autophagy in Trypanosoma brucei: Amino Acid Requirement and Regulation during Different Growth Phases
title_fullStr Autophagy in Trypanosoma brucei: Amino Acid Requirement and Regulation during Different Growth Phases
title_full_unstemmed Autophagy in Trypanosoma brucei: Amino Acid Requirement and Regulation during Different Growth Phases
title_short Autophagy in Trypanosoma brucei: Amino Acid Requirement and Regulation during Different Growth Phases
title_sort autophagy in trypanosoma brucei: amino acid requirement and regulation during different growth phases
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3974859/
https://www.ncbi.nlm.nih.gov/pubmed/24699810
http://dx.doi.org/10.1371/journal.pone.0093875
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