Cargando…

A Proteomic Approach of Bradyrhizobium/Aeschynomene Root and Stem Symbioses Reveals the Importance of the fixA Locus for Symbiosis

Rhizobia are soil bacteria that are able to form symbiosis with plant hosts of the legume family. These associations result in the formation of organs, called nodules in which bacteria fix atmospheric nitrogen to the benefit of the plant. Most of our knowledge on the metabolism and the physiology of...

Descripción completa

Detalles Bibliográficos
Autores principales: Delmotte, Nathanael, Mondy, Samuel, Alunni, Benoit, Fardoux, Joel, Chaintreuil, Clémence, Vorholt, Julia A., Giraud, Eric, Gourion, Benjamin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3975360/
https://www.ncbi.nlm.nih.gov/pubmed/24590127
http://dx.doi.org/10.3390/ijms15033660
_version_ 1782310135762255872
author Delmotte, Nathanael
Mondy, Samuel
Alunni, Benoit
Fardoux, Joel
Chaintreuil, Clémence
Vorholt, Julia A.
Giraud, Eric
Gourion, Benjamin
author_facet Delmotte, Nathanael
Mondy, Samuel
Alunni, Benoit
Fardoux, Joel
Chaintreuil, Clémence
Vorholt, Julia A.
Giraud, Eric
Gourion, Benjamin
author_sort Delmotte, Nathanael
collection PubMed
description Rhizobia are soil bacteria that are able to form symbiosis with plant hosts of the legume family. These associations result in the formation of organs, called nodules in which bacteria fix atmospheric nitrogen to the benefit of the plant. Most of our knowledge on the metabolism and the physiology of the bacteria during symbiosis derives from studying roots nodules of terrestrial plants. Here we used a proteomics approach to investigate the bacterial physiology of photosynthetic Bradyrhizobium sp. ORS278 during the symbiotic process with the semi aquatical plant Aeschynomene indica that forms root and stem nodules. We analyzed the proteomes of bacteria extracted from each type of nodule. First, we analyzed the bacteroid proteome at two different time points and found only minor variation between the bacterial proteomes of 2-week- and 3-week-old nodules. High conservation of the bacteroid proteome was also found when comparing stem nodules and root nodules. Among the stem nodule specific proteins were those related to the phototrophic ability of Bradyrhizobium sp. ORS278. Furthermore, we compared our data with those obtained during an extensive genetic screen previously published. The symbiotic role of four candidate genes which corresponding proteins were found massively produced in the nodules but not identified during this screening was examined. Mutant analysis suggested that in addition to the EtfAB system, the fixA locus is required for symbiotic efficiency.
format Online
Article
Text
id pubmed-3975360
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Molecular Diversity Preservation International (MDPI)
record_format MEDLINE/PubMed
spelling pubmed-39753602014-04-04 A Proteomic Approach of Bradyrhizobium/Aeschynomene Root and Stem Symbioses Reveals the Importance of the fixA Locus for Symbiosis Delmotte, Nathanael Mondy, Samuel Alunni, Benoit Fardoux, Joel Chaintreuil, Clémence Vorholt, Julia A. Giraud, Eric Gourion, Benjamin Int J Mol Sci Communication Rhizobia are soil bacteria that are able to form symbiosis with plant hosts of the legume family. These associations result in the formation of organs, called nodules in which bacteria fix atmospheric nitrogen to the benefit of the plant. Most of our knowledge on the metabolism and the physiology of the bacteria during symbiosis derives from studying roots nodules of terrestrial plants. Here we used a proteomics approach to investigate the bacterial physiology of photosynthetic Bradyrhizobium sp. ORS278 during the symbiotic process with the semi aquatical plant Aeschynomene indica that forms root and stem nodules. We analyzed the proteomes of bacteria extracted from each type of nodule. First, we analyzed the bacteroid proteome at two different time points and found only minor variation between the bacterial proteomes of 2-week- and 3-week-old nodules. High conservation of the bacteroid proteome was also found when comparing stem nodules and root nodules. Among the stem nodule specific proteins were those related to the phototrophic ability of Bradyrhizobium sp. ORS278. Furthermore, we compared our data with those obtained during an extensive genetic screen previously published. The symbiotic role of four candidate genes which corresponding proteins were found massively produced in the nodules but not identified during this screening was examined. Mutant analysis suggested that in addition to the EtfAB system, the fixA locus is required for symbiotic efficiency. Molecular Diversity Preservation International (MDPI) 2014-02-28 /pmc/articles/PMC3975360/ /pubmed/24590127 http://dx.doi.org/10.3390/ijms15033660 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Communication
Delmotte, Nathanael
Mondy, Samuel
Alunni, Benoit
Fardoux, Joel
Chaintreuil, Clémence
Vorholt, Julia A.
Giraud, Eric
Gourion, Benjamin
A Proteomic Approach of Bradyrhizobium/Aeschynomene Root and Stem Symbioses Reveals the Importance of the fixA Locus for Symbiosis
title A Proteomic Approach of Bradyrhizobium/Aeschynomene Root and Stem Symbioses Reveals the Importance of the fixA Locus for Symbiosis
title_full A Proteomic Approach of Bradyrhizobium/Aeschynomene Root and Stem Symbioses Reveals the Importance of the fixA Locus for Symbiosis
title_fullStr A Proteomic Approach of Bradyrhizobium/Aeschynomene Root and Stem Symbioses Reveals the Importance of the fixA Locus for Symbiosis
title_full_unstemmed A Proteomic Approach of Bradyrhizobium/Aeschynomene Root and Stem Symbioses Reveals the Importance of the fixA Locus for Symbiosis
title_short A Proteomic Approach of Bradyrhizobium/Aeschynomene Root and Stem Symbioses Reveals the Importance of the fixA Locus for Symbiosis
title_sort proteomic approach of bradyrhizobium/aeschynomene root and stem symbioses reveals the importance of the fixa locus for symbiosis
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3975360/
https://www.ncbi.nlm.nih.gov/pubmed/24590127
http://dx.doi.org/10.3390/ijms15033660
work_keys_str_mv AT delmottenathanael aproteomicapproachofbradyrhizobiumaeschynomenerootandstemsymbiosesrevealstheimportanceofthefixalocusforsymbiosis
AT mondysamuel aproteomicapproachofbradyrhizobiumaeschynomenerootandstemsymbiosesrevealstheimportanceofthefixalocusforsymbiosis
AT alunnibenoit aproteomicapproachofbradyrhizobiumaeschynomenerootandstemsymbiosesrevealstheimportanceofthefixalocusforsymbiosis
AT fardouxjoel aproteomicapproachofbradyrhizobiumaeschynomenerootandstemsymbiosesrevealstheimportanceofthefixalocusforsymbiosis
AT chaintreuilclemence aproteomicapproachofbradyrhizobiumaeschynomenerootandstemsymbiosesrevealstheimportanceofthefixalocusforsymbiosis
AT vorholtjuliaa aproteomicapproachofbradyrhizobiumaeschynomenerootandstemsymbiosesrevealstheimportanceofthefixalocusforsymbiosis
AT girauderic aproteomicapproachofbradyrhizobiumaeschynomenerootandstemsymbiosesrevealstheimportanceofthefixalocusforsymbiosis
AT gourionbenjamin aproteomicapproachofbradyrhizobiumaeschynomenerootandstemsymbiosesrevealstheimportanceofthefixalocusforsymbiosis
AT delmottenathanael proteomicapproachofbradyrhizobiumaeschynomenerootandstemsymbiosesrevealstheimportanceofthefixalocusforsymbiosis
AT mondysamuel proteomicapproachofbradyrhizobiumaeschynomenerootandstemsymbiosesrevealstheimportanceofthefixalocusforsymbiosis
AT alunnibenoit proteomicapproachofbradyrhizobiumaeschynomenerootandstemsymbiosesrevealstheimportanceofthefixalocusforsymbiosis
AT fardouxjoel proteomicapproachofbradyrhizobiumaeschynomenerootandstemsymbiosesrevealstheimportanceofthefixalocusforsymbiosis
AT chaintreuilclemence proteomicapproachofbradyrhizobiumaeschynomenerootandstemsymbiosesrevealstheimportanceofthefixalocusforsymbiosis
AT vorholtjuliaa proteomicapproachofbradyrhizobiumaeschynomenerootandstemsymbiosesrevealstheimportanceofthefixalocusforsymbiosis
AT girauderic proteomicapproachofbradyrhizobiumaeschynomenerootandstemsymbiosesrevealstheimportanceofthefixalocusforsymbiosis
AT gourionbenjamin proteomicapproachofbradyrhizobiumaeschynomenerootandstemsymbiosesrevealstheimportanceofthefixalocusforsymbiosis