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Multilineage Potential Research of Bovine Amniotic Fluid Mesenchymal Stem Cells

The use of amnion and amniotic fluid (AF) are abundant sources of mesenchymal stem cells (MSCs) that can be harvested at low cost and do not pose ethical conflicts. In human and veterinary research, stem cells derived from these tissues are promising candidates for disease treatment, specifically fo...

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Autores principales: Gao, Yuhua, Zhu, Zhiqiang, Zhao, Yuhua, Hua, Jinlian, Ma, Yuehui, Guan, Weijun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3975362/
https://www.ncbi.nlm.nih.gov/pubmed/24590129
http://dx.doi.org/10.3390/ijms15033698
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author Gao, Yuhua
Zhu, Zhiqiang
Zhao, Yuhua
Hua, Jinlian
Ma, Yuehui
Guan, Weijun
author_facet Gao, Yuhua
Zhu, Zhiqiang
Zhao, Yuhua
Hua, Jinlian
Ma, Yuehui
Guan, Weijun
author_sort Gao, Yuhua
collection PubMed
description The use of amnion and amniotic fluid (AF) are abundant sources of mesenchymal stem cells (MSCs) that can be harvested at low cost and do not pose ethical conflicts. In human and veterinary research, stem cells derived from these tissues are promising candidates for disease treatment, specifically for their plasticity, their reduced immunogenicity, and high anti-inflammatory potential. This work aimed to obtain and characterize bovine amniotic fluid mesenchymal stem cells (AFMSC). The bovine AF from the amniotic cavity of pregnant gilts in the early stages of gestation (3- and 4-m-old bovine embryos) was collected. AFMSCs exhibit a fibroblastic-like morphology only starting from the fourth passage, being heterogeneous during the primary culture. Immunofluorescence results showed that AFMSCs were positive for β-integrin, CD44, CD73 and CD166, but negative for CD34, CD45. Meanwhile, AFMSCs expressed ES cell markers, such as Oct4, and when appropriately induced, are capable of differentiating into ectodermal and mesodermal lineages. This study reinforces the emerging importance of these cells as ideal tools in veterinary medicine; future studies aimed at a deeper evaluation of their immunological properties will allow a better understanding of their role in cellular therapy.
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spelling pubmed-39753622014-04-04 Multilineage Potential Research of Bovine Amniotic Fluid Mesenchymal Stem Cells Gao, Yuhua Zhu, Zhiqiang Zhao, Yuhua Hua, Jinlian Ma, Yuehui Guan, Weijun Int J Mol Sci Article The use of amnion and amniotic fluid (AF) are abundant sources of mesenchymal stem cells (MSCs) that can be harvested at low cost and do not pose ethical conflicts. In human and veterinary research, stem cells derived from these tissues are promising candidates for disease treatment, specifically for their plasticity, their reduced immunogenicity, and high anti-inflammatory potential. This work aimed to obtain and characterize bovine amniotic fluid mesenchymal stem cells (AFMSC). The bovine AF from the amniotic cavity of pregnant gilts in the early stages of gestation (3- and 4-m-old bovine embryos) was collected. AFMSCs exhibit a fibroblastic-like morphology only starting from the fourth passage, being heterogeneous during the primary culture. Immunofluorescence results showed that AFMSCs were positive for β-integrin, CD44, CD73 and CD166, but negative for CD34, CD45. Meanwhile, AFMSCs expressed ES cell markers, such as Oct4, and when appropriately induced, are capable of differentiating into ectodermal and mesodermal lineages. This study reinforces the emerging importance of these cells as ideal tools in veterinary medicine; future studies aimed at a deeper evaluation of their immunological properties will allow a better understanding of their role in cellular therapy. Molecular Diversity Preservation International (MDPI) 2014-02-28 /pmc/articles/PMC3975362/ /pubmed/24590129 http://dx.doi.org/10.3390/ijms15033698 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Gao, Yuhua
Zhu, Zhiqiang
Zhao, Yuhua
Hua, Jinlian
Ma, Yuehui
Guan, Weijun
Multilineage Potential Research of Bovine Amniotic Fluid Mesenchymal Stem Cells
title Multilineage Potential Research of Bovine Amniotic Fluid Mesenchymal Stem Cells
title_full Multilineage Potential Research of Bovine Amniotic Fluid Mesenchymal Stem Cells
title_fullStr Multilineage Potential Research of Bovine Amniotic Fluid Mesenchymal Stem Cells
title_full_unstemmed Multilineage Potential Research of Bovine Amniotic Fluid Mesenchymal Stem Cells
title_short Multilineage Potential Research of Bovine Amniotic Fluid Mesenchymal Stem Cells
title_sort multilineage potential research of bovine amniotic fluid mesenchymal stem cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3975362/
https://www.ncbi.nlm.nih.gov/pubmed/24590129
http://dx.doi.org/10.3390/ijms15033698
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