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Enhanced Anti-Cancer Effect of Snake Venom Activated NK Cells on Lung Cancer Cells by Inactivation of NF-κB

In the present study, we investigated anti-cancer effect of snake venom activated NK cells (NK-92MI) in lung cancer cell lines. We used snake venom (4 μg/ml) treated NK-92MI cells to co-culture with lung cancer cells. There was a further decrease in cancer cell growth up to 65% and 70% in A549 and N...

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Autores principales: Kollipara, Pushpa Saranya, Won, Do Hee, Hwang, Chul Ju, Jung, Yu Yeon, Yoon, Heui Seoung, Park, Mi Hee, Song, Min Jong, Song, Ho Sueb, Hong, Jin Tae
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Applied Pharmacology 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3975473/
https://www.ncbi.nlm.nih.gov/pubmed/24753815
http://dx.doi.org/10.4062/biomolther.2013.103
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author Kollipara, Pushpa Saranya
Won, Do Hee
Hwang, Chul Ju
Jung, Yu Yeon
Yoon, Heui Seoung
Park, Mi Hee
Song, Min Jong
Song, Ho Sueb
Hong, Jin Tae
author_facet Kollipara, Pushpa Saranya
Won, Do Hee
Hwang, Chul Ju
Jung, Yu Yeon
Yoon, Heui Seoung
Park, Mi Hee
Song, Min Jong
Song, Ho Sueb
Hong, Jin Tae
author_sort Kollipara, Pushpa Saranya
collection PubMed
description In the present study, we investigated anti-cancer effect of snake venom activated NK cells (NK-92MI) in lung cancer cell lines. We used snake venom (4 μg/ml) treated NK-92MI cells to co-culture with lung cancer cells. There was a further decrease in cancer cell growth up to 65% and 70% in A549 and NCI-H460 cell lines respectively, whereas 30–40% was decreased in cancer cell growth by snake venom or NK-92MI alone treatment. We further found that the expression of various apoptotic proteins such as that Bax, and cleaved caspase-3 as well as the expression of various death receptor proteins like DR3, DR4 and Fas was also further increased. Moreover, consistent with cancer cell growth inhibition, the DNA binding activity of NF-κB was also further inhibited after treatment of snake venom activated NK-92MI cells. Thus, the present data showed that activated NK cells could further inhibit lung cancer cell growth.
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spelling pubmed-39754732014-04-21 Enhanced Anti-Cancer Effect of Snake Venom Activated NK Cells on Lung Cancer Cells by Inactivation of NF-κB Kollipara, Pushpa Saranya Won, Do Hee Hwang, Chul Ju Jung, Yu Yeon Yoon, Heui Seoung Park, Mi Hee Song, Min Jong Song, Ho Sueb Hong, Jin Tae Biomol Ther (Seoul) Original Article In the present study, we investigated anti-cancer effect of snake venom activated NK cells (NK-92MI) in lung cancer cell lines. We used snake venom (4 μg/ml) treated NK-92MI cells to co-culture with lung cancer cells. There was a further decrease in cancer cell growth up to 65% and 70% in A549 and NCI-H460 cell lines respectively, whereas 30–40% was decreased in cancer cell growth by snake venom or NK-92MI alone treatment. We further found that the expression of various apoptotic proteins such as that Bax, and cleaved caspase-3 as well as the expression of various death receptor proteins like DR3, DR4 and Fas was also further increased. Moreover, consistent with cancer cell growth inhibition, the DNA binding activity of NF-κB was also further inhibited after treatment of snake venom activated NK-92MI cells. Thus, the present data showed that activated NK cells could further inhibit lung cancer cell growth. The Korean Society of Applied Pharmacology 2014-03 /pmc/articles/PMC3975473/ /pubmed/24753815 http://dx.doi.org/10.4062/biomolther.2013.103 Text en Copyright © 2014 The Korean Society of Applied Pharmacology This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Kollipara, Pushpa Saranya
Won, Do Hee
Hwang, Chul Ju
Jung, Yu Yeon
Yoon, Heui Seoung
Park, Mi Hee
Song, Min Jong
Song, Ho Sueb
Hong, Jin Tae
Enhanced Anti-Cancer Effect of Snake Venom Activated NK Cells on Lung Cancer Cells by Inactivation of NF-κB
title Enhanced Anti-Cancer Effect of Snake Venom Activated NK Cells on Lung Cancer Cells by Inactivation of NF-κB
title_full Enhanced Anti-Cancer Effect of Snake Venom Activated NK Cells on Lung Cancer Cells by Inactivation of NF-κB
title_fullStr Enhanced Anti-Cancer Effect of Snake Venom Activated NK Cells on Lung Cancer Cells by Inactivation of NF-κB
title_full_unstemmed Enhanced Anti-Cancer Effect of Snake Venom Activated NK Cells on Lung Cancer Cells by Inactivation of NF-κB
title_short Enhanced Anti-Cancer Effect of Snake Venom Activated NK Cells on Lung Cancer Cells by Inactivation of NF-κB
title_sort enhanced anti-cancer effect of snake venom activated nk cells on lung cancer cells by inactivation of nf-κb
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3975473/
https://www.ncbi.nlm.nih.gov/pubmed/24753815
http://dx.doi.org/10.4062/biomolther.2013.103
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