Cargando…
Small RNAs in the peripheral blood discriminate metastasized from non-metastasized seminoma
BACKGROUND: We aimed to better discriminate metastasized (lymphogen/occult/both combined) from non-metastasized seminoma based on post-transcriptional changes examined in the peripheral blood. METHODS: Total RNAs including small RNAs were isolated from the peripheral blood of patients suffering from...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3975631/ https://www.ncbi.nlm.nih.gov/pubmed/24597607 http://dx.doi.org/10.1186/1476-4598-13-47 |
_version_ | 1782310172209709056 |
---|---|
author | Ruf, Christian G Dinger, Daniela Port, Matthias Schmelz, Hans-Ulrich Wagner, Walter Matthies, Cord Müller-Myhsok, Bertram Meineke, Viktor Abend, Michael |
author_facet | Ruf, Christian G Dinger, Daniela Port, Matthias Schmelz, Hans-Ulrich Wagner, Walter Matthies, Cord Müller-Myhsok, Bertram Meineke, Viktor Abend, Michael |
author_sort | Ruf, Christian G |
collection | PubMed |
description | BACKGROUND: We aimed to better discriminate metastasized (lymphogen/occult/both combined) from non-metastasized seminoma based on post-transcriptional changes examined in the peripheral blood. METHODS: Total RNAs including small RNAs were isolated from the peripheral blood of patients suffering from metastasized testicular tumours (lymphogen, n = 5, clinical stage IIb/c; occult, n = 5, clinical stage I) and non-metastasized patients (n = 5, clinical stage I). Small RNA next generation sequencing (SOLID, Life Technologies) was employed to examine post-transcriptional changes. We searched for small RNAs showing at least 50 reads and a significant ≥ 2-fold difference using peripheral blood small RNAs of non-metastasized tumours as the reference group. Candidate small RNAs were examined in univariate logistic regression analysis and combinations of two small RNAs were further examined using support vector machines. RESULTS: On average 1.3x10(7), 1.2x10(7) and 1.2x10(7) small RNA reads were detectable in non-metastasized, lymphogen and occult metastasized seminoma, respectively of which 73-76% remained after trimming. From these between 80-82% represented annotated reads and 7.2-7.8% (1.6-1.7x10(4)) were annotated small RNA tags. Of them 137 small RNAs showed > 50 reads and a ≥ two-fold difference to the reference. In univariate analysis we detected 33-35 different small RNAs which significantly discriminated lymphogen/occult/combined metastasized from non-metastasized seminoma and among these different comparisons it were the same small RNAs in 44-79%. Many combinations of two of these small RNAs completely discriminated metastasized from non-metastasized seminoma irrespective of the metastasis subtype. CONCLUSIONS: Metastasized (either lymphogen or occult) seminoma can be completely discriminated from non-metastasized seminoma with a combination of two small RNAs measured in the peripheral blood. |
format | Online Article Text |
id | pubmed-3975631 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-39756312014-04-05 Small RNAs in the peripheral blood discriminate metastasized from non-metastasized seminoma Ruf, Christian G Dinger, Daniela Port, Matthias Schmelz, Hans-Ulrich Wagner, Walter Matthies, Cord Müller-Myhsok, Bertram Meineke, Viktor Abend, Michael Mol Cancer Research BACKGROUND: We aimed to better discriminate metastasized (lymphogen/occult/both combined) from non-metastasized seminoma based on post-transcriptional changes examined in the peripheral blood. METHODS: Total RNAs including small RNAs were isolated from the peripheral blood of patients suffering from metastasized testicular tumours (lymphogen, n = 5, clinical stage IIb/c; occult, n = 5, clinical stage I) and non-metastasized patients (n = 5, clinical stage I). Small RNA next generation sequencing (SOLID, Life Technologies) was employed to examine post-transcriptional changes. We searched for small RNAs showing at least 50 reads and a significant ≥ 2-fold difference using peripheral blood small RNAs of non-metastasized tumours as the reference group. Candidate small RNAs were examined in univariate logistic regression analysis and combinations of two small RNAs were further examined using support vector machines. RESULTS: On average 1.3x10(7), 1.2x10(7) and 1.2x10(7) small RNA reads were detectable in non-metastasized, lymphogen and occult metastasized seminoma, respectively of which 73-76% remained after trimming. From these between 80-82% represented annotated reads and 7.2-7.8% (1.6-1.7x10(4)) were annotated small RNA tags. Of them 137 small RNAs showed > 50 reads and a ≥ two-fold difference to the reference. In univariate analysis we detected 33-35 different small RNAs which significantly discriminated lymphogen/occult/combined metastasized from non-metastasized seminoma and among these different comparisons it were the same small RNAs in 44-79%. Many combinations of two of these small RNAs completely discriminated metastasized from non-metastasized seminoma irrespective of the metastasis subtype. CONCLUSIONS: Metastasized (either lymphogen or occult) seminoma can be completely discriminated from non-metastasized seminoma with a combination of two small RNAs measured in the peripheral blood. BioMed Central 2014-03-06 /pmc/articles/PMC3975631/ /pubmed/24597607 http://dx.doi.org/10.1186/1476-4598-13-47 Text en Copyright © 2014 Ruf et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Ruf, Christian G Dinger, Daniela Port, Matthias Schmelz, Hans-Ulrich Wagner, Walter Matthies, Cord Müller-Myhsok, Bertram Meineke, Viktor Abend, Michael Small RNAs in the peripheral blood discriminate metastasized from non-metastasized seminoma |
title | Small RNAs in the peripheral blood discriminate metastasized from non-metastasized seminoma |
title_full | Small RNAs in the peripheral blood discriminate metastasized from non-metastasized seminoma |
title_fullStr | Small RNAs in the peripheral blood discriminate metastasized from non-metastasized seminoma |
title_full_unstemmed | Small RNAs in the peripheral blood discriminate metastasized from non-metastasized seminoma |
title_short | Small RNAs in the peripheral blood discriminate metastasized from non-metastasized seminoma |
title_sort | small rnas in the peripheral blood discriminate metastasized from non-metastasized seminoma |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3975631/ https://www.ncbi.nlm.nih.gov/pubmed/24597607 http://dx.doi.org/10.1186/1476-4598-13-47 |
work_keys_str_mv | AT rufchristiang smallrnasintheperipheralblooddiscriminatemetastasizedfromnonmetastasizedseminoma AT dingerdaniela smallrnasintheperipheralblooddiscriminatemetastasizedfromnonmetastasizedseminoma AT portmatthias smallrnasintheperipheralblooddiscriminatemetastasizedfromnonmetastasizedseminoma AT schmelzhansulrich smallrnasintheperipheralblooddiscriminatemetastasizedfromnonmetastasizedseminoma AT wagnerwalter smallrnasintheperipheralblooddiscriminatemetastasizedfromnonmetastasizedseminoma AT matthiescord smallrnasintheperipheralblooddiscriminatemetastasizedfromnonmetastasizedseminoma AT mullermyhsokbertram smallrnasintheperipheralblooddiscriminatemetastasizedfromnonmetastasizedseminoma AT meinekeviktor smallrnasintheperipheralblooddiscriminatemetastasizedfromnonmetastasizedseminoma AT abendmichael smallrnasintheperipheralblooddiscriminatemetastasizedfromnonmetastasizedseminoma |