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Optimized conditions for successful transfection of human endothelial cells with in vitro synthesized and modified mRNA for induction of protein expression

BACKGROUND: The induction of protein synthesis by exogenous delivery of coding synthetic mRNA in desired cells is an auspicious strategy in the fields of basic cell biology, regenerative medicine, treatment of diseases, and reprogramming of cells. Here, we produced modified messenger RNA (mRNA) with...

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Detalles Bibliográficos
Autores principales: Avci-Adali, Meltem, Behring, Andreas, Keller, Timea, Krajewski, Stefanie, Schlensak, Christian, Wendel, Hans Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3975882/
https://www.ncbi.nlm.nih.gov/pubmed/24581116
http://dx.doi.org/10.1186/1754-1611-8-8