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A Proteomic View to Characterize the Effect of Chitosan Nanoparticle to Hepatic Cells: Is Chitosan Nanoparticle an Enhancer of PI3K/AKT1/mTOR Pathway?

Chitosan nanoparticle, a biocompatible material, was used as a potential drug delivery system widely. Our current investigation studies were the bioeffects of the chitosan nanoparticle uptake by liver cells. In this experiment, the characterizations of chitosan nanoparticles were measured by transmi...

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Detalles Bibliográficos
Autores principales: Yang, Ming-Hui, Yuan, Shyng-Shiou, Huang, Ying-Fong, Lin, Po-Chiao, Lu, Chi-Yu, Chung, Tze-Wen, Tyan, Yu-Chang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3976794/
https://www.ncbi.nlm.nih.gov/pubmed/24757677
http://dx.doi.org/10.1155/2014/789591
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author Yang, Ming-Hui
Yuan, Shyng-Shiou
Huang, Ying-Fong
Lin, Po-Chiao
Lu, Chi-Yu
Chung, Tze-Wen
Tyan, Yu-Chang
author_facet Yang, Ming-Hui
Yuan, Shyng-Shiou
Huang, Ying-Fong
Lin, Po-Chiao
Lu, Chi-Yu
Chung, Tze-Wen
Tyan, Yu-Chang
author_sort Yang, Ming-Hui
collection PubMed
description Chitosan nanoparticle, a biocompatible material, was used as a potential drug delivery system widely. Our current investigation studies were the bioeffects of the chitosan nanoparticle uptake by liver cells. In this experiment, the characterizations of chitosan nanoparticles were measured by transmission electron microscopy and particle size analyzer. The average size of the chitosan nanoparticle was 224.6 ± 11.2 nm, and the average zeta potential was +14.08 ± 0.7 mV. Moreover, using proteomic approaches to analyze the differential protein expression patterns resulted from the chitosan nanoparticle uptaken by HepG2 and CCL-13 cells identified several proteins involved in the PI3K/AKT1/mTOR pathway. Our experimental results have demonstrated that the chitosan nanoparticle may involve in the liver cancer cell metastasis and proliferation.
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spelling pubmed-39767942014-04-22 A Proteomic View to Characterize the Effect of Chitosan Nanoparticle to Hepatic Cells: Is Chitosan Nanoparticle an Enhancer of PI3K/AKT1/mTOR Pathway? Yang, Ming-Hui Yuan, Shyng-Shiou Huang, Ying-Fong Lin, Po-Chiao Lu, Chi-Yu Chung, Tze-Wen Tyan, Yu-Chang Biomed Res Int Research Article Chitosan nanoparticle, a biocompatible material, was used as a potential drug delivery system widely. Our current investigation studies were the bioeffects of the chitosan nanoparticle uptake by liver cells. In this experiment, the characterizations of chitosan nanoparticles were measured by transmission electron microscopy and particle size analyzer. The average size of the chitosan nanoparticle was 224.6 ± 11.2 nm, and the average zeta potential was +14.08 ± 0.7 mV. Moreover, using proteomic approaches to analyze the differential protein expression patterns resulted from the chitosan nanoparticle uptaken by HepG2 and CCL-13 cells identified several proteins involved in the PI3K/AKT1/mTOR pathway. Our experimental results have demonstrated that the chitosan nanoparticle may involve in the liver cancer cell metastasis and proliferation. Hindawi Publishing Corporation 2014 2014-03-16 /pmc/articles/PMC3976794/ /pubmed/24757677 http://dx.doi.org/10.1155/2014/789591 Text en Copyright © 2014 Ming-Hui Yang et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Yang, Ming-Hui
Yuan, Shyng-Shiou
Huang, Ying-Fong
Lin, Po-Chiao
Lu, Chi-Yu
Chung, Tze-Wen
Tyan, Yu-Chang
A Proteomic View to Characterize the Effect of Chitosan Nanoparticle to Hepatic Cells: Is Chitosan Nanoparticle an Enhancer of PI3K/AKT1/mTOR Pathway?
title A Proteomic View to Characterize the Effect of Chitosan Nanoparticle to Hepatic Cells: Is Chitosan Nanoparticle an Enhancer of PI3K/AKT1/mTOR Pathway?
title_full A Proteomic View to Characterize the Effect of Chitosan Nanoparticle to Hepatic Cells: Is Chitosan Nanoparticle an Enhancer of PI3K/AKT1/mTOR Pathway?
title_fullStr A Proteomic View to Characterize the Effect of Chitosan Nanoparticle to Hepatic Cells: Is Chitosan Nanoparticle an Enhancer of PI3K/AKT1/mTOR Pathway?
title_full_unstemmed A Proteomic View to Characterize the Effect of Chitosan Nanoparticle to Hepatic Cells: Is Chitosan Nanoparticle an Enhancer of PI3K/AKT1/mTOR Pathway?
title_short A Proteomic View to Characterize the Effect of Chitosan Nanoparticle to Hepatic Cells: Is Chitosan Nanoparticle an Enhancer of PI3K/AKT1/mTOR Pathway?
title_sort proteomic view to characterize the effect of chitosan nanoparticle to hepatic cells: is chitosan nanoparticle an enhancer of pi3k/akt1/mtor pathway?
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3976794/
https://www.ncbi.nlm.nih.gov/pubmed/24757677
http://dx.doi.org/10.1155/2014/789591
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