Nanoliter Segmented-Flow Sampling Mass Spectrometry with Online Compartmentalization

[Image: see text] We report a microfluidic device, using segmented flow in a two-phase system of immiscible liquids, which delivers aqueous droplets into a modified commercial mass spectrometer. The interface coupling the microfluidics to the mass spectrometer achieves up to 96% sample transfer effi...

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Autores principales: Volný, Michael, Rolfs, Joelle, Hakimi, Bejan, Fryčák, Petr, Schneider, Thomas, Liu, Dingsheng, Yen, Gloria, Chiu, Daniel T., Tureček, František
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2014
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3977581/
https://www.ncbi.nlm.nih.gov/pubmed/24625074
http://dx.doi.org/10.1021/ac500365r
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author Volný, Michael
Rolfs, Joelle
Hakimi, Bejan
Fryčák, Petr
Schneider, Thomas
Liu, Dingsheng
Yen, Gloria
Chiu, Daniel T.
Tureček, František
author_facet Volný, Michael
Rolfs, Joelle
Hakimi, Bejan
Fryčák, Petr
Schneider, Thomas
Liu, Dingsheng
Yen, Gloria
Chiu, Daniel T.
Tureček, František
author_sort Volný, Michael
collection PubMed
description [Image: see text] We report a microfluidic device, using segmented flow in a two-phase system of immiscible liquids, which delivers aqueous droplets into a modified commercial mass spectrometer. The interface coupling the microfluidics to the mass spectrometer achieves up to 96% sample transfer efficiency to the vacuum chamber. Sample ionization is assisted by multipass infrared laser beam in the interface. The system achieves low femtomole detection limits of several analytes ranging from drugs to proteins. Sample ionization in this segmented-flow sampling was found to be remarkably insensitive to the presence of buffer salts and other matrices.
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spelling pubmed-39775812015-03-13 Nanoliter Segmented-Flow Sampling Mass Spectrometry with Online Compartmentalization Volný, Michael Rolfs, Joelle Hakimi, Bejan Fryčák, Petr Schneider, Thomas Liu, Dingsheng Yen, Gloria Chiu, Daniel T. Tureček, František Anal Chem [Image: see text] We report a microfluidic device, using segmented flow in a two-phase system of immiscible liquids, which delivers aqueous droplets into a modified commercial mass spectrometer. The interface coupling the microfluidics to the mass spectrometer achieves up to 96% sample transfer efficiency to the vacuum chamber. Sample ionization is assisted by multipass infrared laser beam in the interface. The system achieves low femtomole detection limits of several analytes ranging from drugs to proteins. Sample ionization in this segmented-flow sampling was found to be remarkably insensitive to the presence of buffer salts and other matrices. American Chemical Society 2014-03-13 2014-04-01 /pmc/articles/PMC3977581/ /pubmed/24625074 http://dx.doi.org/10.1021/ac500365r Text en Copyright © 2014 American Chemical Society
spellingShingle Volný, Michael
Rolfs, Joelle
Hakimi, Bejan
Fryčák, Petr
Schneider, Thomas
Liu, Dingsheng
Yen, Gloria
Chiu, Daniel T.
Tureček, František
Nanoliter Segmented-Flow Sampling Mass Spectrometry with Online Compartmentalization
title Nanoliter Segmented-Flow Sampling Mass Spectrometry with Online Compartmentalization
title_full Nanoliter Segmented-Flow Sampling Mass Spectrometry with Online Compartmentalization
title_fullStr Nanoliter Segmented-Flow Sampling Mass Spectrometry with Online Compartmentalization
title_full_unstemmed Nanoliter Segmented-Flow Sampling Mass Spectrometry with Online Compartmentalization
title_short Nanoliter Segmented-Flow Sampling Mass Spectrometry with Online Compartmentalization
title_sort nanoliter segmented-flow sampling mass spectrometry with online compartmentalization
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3977581/
https://www.ncbi.nlm.nih.gov/pubmed/24625074
http://dx.doi.org/10.1021/ac500365r
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