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Rapid single-colony whole-genome sequencing of bacterial pathogens

OBJECTIVES: As a result of the introduction of rapid benchtop sequencers, the time required to subculture a bacterial pathogen to extract sufficient DNA for library preparation can now exceed the time to sequence said DNA. We have eliminated this rate-limiting step by developing a protocol to genera...

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Detalles Bibliográficos
Autores principales:	Köser, Claudio U., Fraser, Louise J., Ioannou, Avgousta, Becq, Jennifer, Ellington, Matthew J., Holden, Matthew T. G., Reuter, Sandra, Török, M. Estée, Bentley, Stephen D., Parkhill, Julian, Gormley, Niall A., Smith, Geoffrey P., Peacock, Sharon J.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2014
Materias:	Original Research
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3977605/ https://www.ncbi.nlm.nih.gov/pubmed/24370932 http://dx.doi.org/10.1093/jac/dkt494

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