Tos17 rice element: incomplete but effective

BACKGROUND: Tos17 was the first LTR retrotransposon (Copia) described as active in cultivated rice, and is present in two copies in the genome of the sequenced Nipponbare variety. Only the chromosome 7 copy is active and able to retrotranspose, at least during in vitro culture, and this ability was...

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Detalles Bibliográficos
Autor principal: Sabot, Francois
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3977881/
https://www.ncbi.nlm.nih.gov/pubmed/24690462
http://dx.doi.org/10.1186/1759-8753-5-10
Descripción
Sumario:BACKGROUND: Tos17 was the first LTR retrotransposon (Copia) described as active in cultivated rice, and is present in two copies in the genome of the sequenced Nipponbare variety. Only the chromosome 7 copy is active and able to retrotranspose, at least during in vitro culture, and this ability was widely used in insertional mutagenesis assays. RESULTS: Here the structure of the active Tos17 was thoroughly annotated using a set of bioinformatic analyses. CONCLUSIONS: Unexpectedly, Tos17 appears to be a non-autonomous LTR retrotransposon, lacking the gag sequence and thus unable to transpose by itself.

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