Cargando…
Noninvasive Imaging of In Vivo MuRF1 Expression during Muscle Atrophy
Numerous human diseases can lead to atrophy of skeletal muscle, and loss of this tissue has been correlated with increased mortality and morbidity rates. Clinically addressing muscle atrophy remains an unmet medical need, and the development of preclinical tools to assist drug discovery and basic re...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3977994/ https://www.ncbi.nlm.nih.gov/pubmed/24710205 http://dx.doi.org/10.1371/journal.pone.0094032 |
_version_ | 1782310492352544768 |
---|---|
author | Li, Wei Claypool, Mark D. Friera, Annabelle M. McLaughlin, John Baltgalvis, Kristen A. Smith, Ira J. Kinoshita, Taisei White, Kathy Lang, Wayne Godinez, Guillermo Payan, Donald G. Kinsella, Todd M. |
author_facet | Li, Wei Claypool, Mark D. Friera, Annabelle M. McLaughlin, John Baltgalvis, Kristen A. Smith, Ira J. Kinoshita, Taisei White, Kathy Lang, Wayne Godinez, Guillermo Payan, Donald G. Kinsella, Todd M. |
author_sort | Li, Wei |
collection | PubMed |
description | Numerous human diseases can lead to atrophy of skeletal muscle, and loss of this tissue has been correlated with increased mortality and morbidity rates. Clinically addressing muscle atrophy remains an unmet medical need, and the development of preclinical tools to assist drug discovery and basic research in this effort is important for advancing this goal. In this report, we describe the development of a bioluminescent gene reporter rat, based on the zinc finger nuclease-targeted insertion of a bicistronic luciferase reporter into the 3′ untranslated region of a muscle specific E3 ubiquitin ligase gene, MuRF1 (Trim63). In longitudinal studies, we noninvasively assess atrophy-related expression of this reporter in three distinct models of muscle loss (sciatic denervation, hindlimb unloading and dexamethasone-treatment) and show that these animals are capable of generating refined detail on in vivo MuRF1 expression with high temporal and anatomical resolution. |
format | Online Article Text |
id | pubmed-3977994 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-39779942014-04-11 Noninvasive Imaging of In Vivo MuRF1 Expression during Muscle Atrophy Li, Wei Claypool, Mark D. Friera, Annabelle M. McLaughlin, John Baltgalvis, Kristen A. Smith, Ira J. Kinoshita, Taisei White, Kathy Lang, Wayne Godinez, Guillermo Payan, Donald G. Kinsella, Todd M. PLoS One Research Article Numerous human diseases can lead to atrophy of skeletal muscle, and loss of this tissue has been correlated with increased mortality and morbidity rates. Clinically addressing muscle atrophy remains an unmet medical need, and the development of preclinical tools to assist drug discovery and basic research in this effort is important for advancing this goal. In this report, we describe the development of a bioluminescent gene reporter rat, based on the zinc finger nuclease-targeted insertion of a bicistronic luciferase reporter into the 3′ untranslated region of a muscle specific E3 ubiquitin ligase gene, MuRF1 (Trim63). In longitudinal studies, we noninvasively assess atrophy-related expression of this reporter in three distinct models of muscle loss (sciatic denervation, hindlimb unloading and dexamethasone-treatment) and show that these animals are capable of generating refined detail on in vivo MuRF1 expression with high temporal and anatomical resolution. Public Library of Science 2014-04-07 /pmc/articles/PMC3977994/ /pubmed/24710205 http://dx.doi.org/10.1371/journal.pone.0094032 Text en © 2014 Li et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Li, Wei Claypool, Mark D. Friera, Annabelle M. McLaughlin, John Baltgalvis, Kristen A. Smith, Ira J. Kinoshita, Taisei White, Kathy Lang, Wayne Godinez, Guillermo Payan, Donald G. Kinsella, Todd M. Noninvasive Imaging of In Vivo MuRF1 Expression during Muscle Atrophy |
title | Noninvasive Imaging of In Vivo MuRF1 Expression during Muscle Atrophy |
title_full | Noninvasive Imaging of In Vivo MuRF1 Expression during Muscle Atrophy |
title_fullStr | Noninvasive Imaging of In Vivo MuRF1 Expression during Muscle Atrophy |
title_full_unstemmed | Noninvasive Imaging of In Vivo MuRF1 Expression during Muscle Atrophy |
title_short | Noninvasive Imaging of In Vivo MuRF1 Expression during Muscle Atrophy |
title_sort | noninvasive imaging of in vivo murf1 expression during muscle atrophy |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3977994/ https://www.ncbi.nlm.nih.gov/pubmed/24710205 http://dx.doi.org/10.1371/journal.pone.0094032 |
work_keys_str_mv | AT liwei noninvasiveimagingofinvivomurf1expressionduringmuscleatrophy AT claypoolmarkd noninvasiveimagingofinvivomurf1expressionduringmuscleatrophy AT frieraannabellem noninvasiveimagingofinvivomurf1expressionduringmuscleatrophy AT mclaughlinjohn noninvasiveimagingofinvivomurf1expressionduringmuscleatrophy AT baltgalviskristena noninvasiveimagingofinvivomurf1expressionduringmuscleatrophy AT smithiraj noninvasiveimagingofinvivomurf1expressionduringmuscleatrophy AT kinoshitataisei noninvasiveimagingofinvivomurf1expressionduringmuscleatrophy AT whitekathy noninvasiveimagingofinvivomurf1expressionduringmuscleatrophy AT langwayne noninvasiveimagingofinvivomurf1expressionduringmuscleatrophy AT godinezguillermo noninvasiveimagingofinvivomurf1expressionduringmuscleatrophy AT payandonaldg noninvasiveimagingofinvivomurf1expressionduringmuscleatrophy AT kinsellatoddm noninvasiveimagingofinvivomurf1expressionduringmuscleatrophy |