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Noninvasive Imaging of In Vivo MuRF1 Expression during Muscle Atrophy

Numerous human diseases can lead to atrophy of skeletal muscle, and loss of this tissue has been correlated with increased mortality and morbidity rates. Clinically addressing muscle atrophy remains an unmet medical need, and the development of preclinical tools to assist drug discovery and basic re...

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Autores principales: Li, Wei, Claypool, Mark D., Friera, Annabelle M., McLaughlin, John, Baltgalvis, Kristen A., Smith, Ira J., Kinoshita, Taisei, White, Kathy, Lang, Wayne, Godinez, Guillermo, Payan, Donald G., Kinsella, Todd M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3977994/
https://www.ncbi.nlm.nih.gov/pubmed/24710205
http://dx.doi.org/10.1371/journal.pone.0094032
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author Li, Wei
Claypool, Mark D.
Friera, Annabelle M.
McLaughlin, John
Baltgalvis, Kristen A.
Smith, Ira J.
Kinoshita, Taisei
White, Kathy
Lang, Wayne
Godinez, Guillermo
Payan, Donald G.
Kinsella, Todd M.
author_facet Li, Wei
Claypool, Mark D.
Friera, Annabelle M.
McLaughlin, John
Baltgalvis, Kristen A.
Smith, Ira J.
Kinoshita, Taisei
White, Kathy
Lang, Wayne
Godinez, Guillermo
Payan, Donald G.
Kinsella, Todd M.
author_sort Li, Wei
collection PubMed
description Numerous human diseases can lead to atrophy of skeletal muscle, and loss of this tissue has been correlated with increased mortality and morbidity rates. Clinically addressing muscle atrophy remains an unmet medical need, and the development of preclinical tools to assist drug discovery and basic research in this effort is important for advancing this goal. In this report, we describe the development of a bioluminescent gene reporter rat, based on the zinc finger nuclease-targeted insertion of a bicistronic luciferase reporter into the 3′ untranslated region of a muscle specific E3 ubiquitin ligase gene, MuRF1 (Trim63). In longitudinal studies, we noninvasively assess atrophy-related expression of this reporter in three distinct models of muscle loss (sciatic denervation, hindlimb unloading and dexamethasone-treatment) and show that these animals are capable of generating refined detail on in vivo MuRF1 expression with high temporal and anatomical resolution.
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spelling pubmed-39779942014-04-11 Noninvasive Imaging of In Vivo MuRF1 Expression during Muscle Atrophy Li, Wei Claypool, Mark D. Friera, Annabelle M. McLaughlin, John Baltgalvis, Kristen A. Smith, Ira J. Kinoshita, Taisei White, Kathy Lang, Wayne Godinez, Guillermo Payan, Donald G. Kinsella, Todd M. PLoS One Research Article Numerous human diseases can lead to atrophy of skeletal muscle, and loss of this tissue has been correlated with increased mortality and morbidity rates. Clinically addressing muscle atrophy remains an unmet medical need, and the development of preclinical tools to assist drug discovery and basic research in this effort is important for advancing this goal. In this report, we describe the development of a bioluminescent gene reporter rat, based on the zinc finger nuclease-targeted insertion of a bicistronic luciferase reporter into the 3′ untranslated region of a muscle specific E3 ubiquitin ligase gene, MuRF1 (Trim63). In longitudinal studies, we noninvasively assess atrophy-related expression of this reporter in three distinct models of muscle loss (sciatic denervation, hindlimb unloading and dexamethasone-treatment) and show that these animals are capable of generating refined detail on in vivo MuRF1 expression with high temporal and anatomical resolution. Public Library of Science 2014-04-07 /pmc/articles/PMC3977994/ /pubmed/24710205 http://dx.doi.org/10.1371/journal.pone.0094032 Text en © 2014 Li et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Li, Wei
Claypool, Mark D.
Friera, Annabelle M.
McLaughlin, John
Baltgalvis, Kristen A.
Smith, Ira J.
Kinoshita, Taisei
White, Kathy
Lang, Wayne
Godinez, Guillermo
Payan, Donald G.
Kinsella, Todd M.
Noninvasive Imaging of In Vivo MuRF1 Expression during Muscle Atrophy
title Noninvasive Imaging of In Vivo MuRF1 Expression during Muscle Atrophy
title_full Noninvasive Imaging of In Vivo MuRF1 Expression during Muscle Atrophy
title_fullStr Noninvasive Imaging of In Vivo MuRF1 Expression during Muscle Atrophy
title_full_unstemmed Noninvasive Imaging of In Vivo MuRF1 Expression during Muscle Atrophy
title_short Noninvasive Imaging of In Vivo MuRF1 Expression during Muscle Atrophy
title_sort noninvasive imaging of in vivo murf1 expression during muscle atrophy
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3977994/
https://www.ncbi.nlm.nih.gov/pubmed/24710205
http://dx.doi.org/10.1371/journal.pone.0094032
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