Functional DNA quantification guides accurate next-generation sequencing mutation detection in formalin-fixed, paraffin-embedded tumor biopsies

The formalin-fixed, paraffin-embedded (FFPE) biopsy is a challenging sample for molecular assays such as targeted next-generation sequencing (NGS). We compared three methods for FFPE DNA quantification, including a novel PCR assay (‘QFI-PCR’) that measures the absolute copy number of amplifiable DNA...

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Detalles Bibliográficos
Autores principales: Sah, Sachin, Chen, Liangjing, Houghton, Jeffrey, Kemppainen, Jon, Marko, Adam C, Zeigler, Robert, Latham, Gary J
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3978876/
https://www.ncbi.nlm.nih.gov/pubmed/24001039
http://dx.doi.org/10.1186/gm481

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