Cargando…

Delivery of endocytosed proteins to the cell–division plane requires change of pathway from recycling to secretion

Membrane trafficking is essential to fundamental processes in eukaryotic life, including cell growth and division. In plant cytokinesis, post-Golgi trafficking mediates a massive flow of vesicles that form the partitioning membrane but its regulation remains poorly understood. Here, we identify func...

Descripción completa

Detalles Bibliográficos
Autores principales: Richter, Sandra, Kientz, Marika, Brumm, Sabine, Nielsen, Mads Eggert, Park, Misoon, Gavidia, Richard, Krause, Cornelia, Voss, Ute, Beckmann, Hauke, Mayer, Ulrike, Stierhof, York-Dieter, Jürgens, Gerd
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3979144/
https://www.ncbi.nlm.nih.gov/pubmed/24714496
http://dx.doi.org/10.7554/eLife.02131
Descripción
Sumario:Membrane trafficking is essential to fundamental processes in eukaryotic life, including cell growth and division. In plant cytokinesis, post-Golgi trafficking mediates a massive flow of vesicles that form the partitioning membrane but its regulation remains poorly understood. Here, we identify functionally redundant Arabidopsis ARF guanine-nucleotide exchange factors (ARF-GEFs) BIG1–BIG4 as regulators of post-Golgi trafficking, mediating late secretion from the trans-Golgi network but not recycling of endocytosed proteins to the plasma membrane, although the TGN also functions as an early endosome in plants. In contrast, BIG1-4 are absolutely required for trafficking of both endocytosed and newly synthesized proteins to the cell–division plane during cytokinesis, counteracting recycling to the plasma membrane. This change from recycling to secretory trafficking pathway mediated by ARF-GEFs confers specificity of cargo delivery to the division plane and might thus ensure that the partitioning membrane is completed on time in the absence of a cytokinesis-interphase checkpoint. DOI: http://dx.doi.org/10.7554/eLife.02131.001