Helvolic acid, an antibacterial nortriterpenoid from a fungal endophyte, Xylaria sp. of orchid Anoectochilus setaceus endemic to Sri Lanka

An endophytic fungus was isolated from surface sterilized leaf segments of Anoectochilus setaceus, an orchid endemic to Sri Lanka, and was identified as Xylaria sp. by morphological characters and DNA sequencing. Bioassay-guided chromatographic fractionation of the organic extract of a laboratory cu...

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Detalles Bibliográficos
Autores principales: Ratnaweera, Pamoda B., Williams, David E., de Silva, E. Dilip, Wijesundera, Ravi L.C., Dalisay, Doralyn S., Andersen, Raymond J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3979440/
https://www.ncbi.nlm.nih.gov/pubmed/24772371
http://dx.doi.org/10.1080/21501203.2014.892905
Descripción
Sumario:An endophytic fungus was isolated from surface sterilized leaf segments of Anoectochilus setaceus, an orchid endemic to Sri Lanka, and was identified as Xylaria sp. by morphological characters and DNA sequencing. Bioassay-guided chromatographic fractionation of the organic extract of a laboratory culture of this fungus led to the isolation of the known antibacterial helvolic acid. Helvolic acid was active against the Gram-positive bacteria, Bacillus subtilis [minimal inhibitory concentrations (MIC), 2 μg mL(−1)] and methicillin-resistant Staphylococcus aureus (MIC, 4 μg mL(−1)).

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