Helvolic acid, an antibacterial nortriterpenoid from a fungal endophyte, Xylaria sp. of orchid Anoectochilus setaceus endemic to Sri Lanka

An endophytic fungus was isolated from surface sterilized leaf segments of Anoectochilus setaceus, an orchid endemic to Sri Lanka, and was identified as Xylaria sp. by morphological characters and DNA sequencing. Bioassay-guided chromatographic fractionation of the organic extract of a laboratory cu...

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Autores principales: Ratnaweera, Pamoda B., Williams, David E., de Silva, E. Dilip, Wijesundera, Ravi L.C., Dalisay, Doralyn S., Andersen, Raymond J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3979440/
https://www.ncbi.nlm.nih.gov/pubmed/24772371
http://dx.doi.org/10.1080/21501203.2014.892905
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author Ratnaweera, Pamoda B.
Williams, David E.
de Silva, E. Dilip
Wijesundera, Ravi L.C.
Dalisay, Doralyn S.
Andersen, Raymond J.
author_facet Ratnaweera, Pamoda B.
Williams, David E.
de Silva, E. Dilip
Wijesundera, Ravi L.C.
Dalisay, Doralyn S.
Andersen, Raymond J.
author_sort Ratnaweera, Pamoda B.
collection PubMed
description An endophytic fungus was isolated from surface sterilized leaf segments of Anoectochilus setaceus, an orchid endemic to Sri Lanka, and was identified as Xylaria sp. by morphological characters and DNA sequencing. Bioassay-guided chromatographic fractionation of the organic extract of a laboratory culture of this fungus led to the isolation of the known antibacterial helvolic acid. Helvolic acid was active against the Gram-positive bacteria, Bacillus subtilis [minimal inhibitory concentrations (MIC), 2 μg mL(−1)] and methicillin-resistant Staphylococcus aureus (MIC, 4 μg mL(−1)).
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spelling pubmed-39794402014-04-25 Helvolic acid, an antibacterial nortriterpenoid from a fungal endophyte, Xylaria sp. of orchid Anoectochilus setaceus endemic to Sri Lanka Ratnaweera, Pamoda B. Williams, David E. de Silva, E. Dilip Wijesundera, Ravi L.C. Dalisay, Doralyn S. Andersen, Raymond J. Mycology Research Article An endophytic fungus was isolated from surface sterilized leaf segments of Anoectochilus setaceus, an orchid endemic to Sri Lanka, and was identified as Xylaria sp. by morphological characters and DNA sequencing. Bioassay-guided chromatographic fractionation of the organic extract of a laboratory culture of this fungus led to the isolation of the known antibacterial helvolic acid. Helvolic acid was active against the Gram-positive bacteria, Bacillus subtilis [minimal inhibitory concentrations (MIC), 2 μg mL(−1)] and methicillin-resistant Staphylococcus aureus (MIC, 4 μg mL(−1)). Taylor & Francis 2014-03-25 2014-03 /pmc/articles/PMC3979440/ /pubmed/24772371 http://dx.doi.org/10.1080/21501203.2014.892905 Text en © The Author(s). Published by Taylor & Francis. http://www.informaworld.com/mpp/uploads/iopenaccess_tcs.pdf This is an open access article distributed under the Supplemental Terms and Conditions for iOpenAccess articles published in Taylor & Francis journals (http://www.informaworld.com/mpp/uploads/iopenaccess_tcs.pdf) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. This is an open-access article distributed under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/3.0/, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.
spellingShingle Research Article
Ratnaweera, Pamoda B.
Williams, David E.
de Silva, E. Dilip
Wijesundera, Ravi L.C.
Dalisay, Doralyn S.
Andersen, Raymond J.
Helvolic acid, an antibacterial nortriterpenoid from a fungal endophyte, Xylaria sp. of orchid Anoectochilus setaceus endemic to Sri Lanka
title Helvolic acid, an antibacterial nortriterpenoid from a fungal endophyte, Xylaria sp. of orchid Anoectochilus setaceus endemic to Sri Lanka
title_full Helvolic acid, an antibacterial nortriterpenoid from a fungal endophyte, Xylaria sp. of orchid Anoectochilus setaceus endemic to Sri Lanka
title_fullStr Helvolic acid, an antibacterial nortriterpenoid from a fungal endophyte, Xylaria sp. of orchid Anoectochilus setaceus endemic to Sri Lanka
title_full_unstemmed Helvolic acid, an antibacterial nortriterpenoid from a fungal endophyte, Xylaria sp. of orchid Anoectochilus setaceus endemic to Sri Lanka
title_short Helvolic acid, an antibacterial nortriterpenoid from a fungal endophyte, Xylaria sp. of orchid Anoectochilus setaceus endemic to Sri Lanka
title_sort helvolic acid, an antibacterial nortriterpenoid from a fungal endophyte, xylaria sp. of orchid anoectochilus setaceus endemic to sri lanka
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3979440/
https://www.ncbi.nlm.nih.gov/pubmed/24772371
http://dx.doi.org/10.1080/21501203.2014.892905
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