Cargando…

Laminaria japonica Extract, an Inhibitor of Clavibater michiganense Subsp. Sepedonicum

Bacterial ring rot of potato is one of the most serious potato plant and tuber diseases. Laminaria japonica extract was investigated for its antimicrobial activity against Clavibater michiganense subsp. sepedonicum (Spieckermann & Kotthoff) Davis et al., the causative agent of bacterial ring rot...

Descripción completa

Detalles Bibliográficos
Autores principales: Cai, Jin, Feng, Jia, Xie, Shulian, Wang, Feipeng, Xu, Qiufeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3979835/
https://www.ncbi.nlm.nih.gov/pubmed/24714388
http://dx.doi.org/10.1371/journal.pone.0094329
_version_ 1782310772458651648
author Cai, Jin
Feng, Jia
Xie, Shulian
Wang, Feipeng
Xu, Qiufeng
author_facet Cai, Jin
Feng, Jia
Xie, Shulian
Wang, Feipeng
Xu, Qiufeng
author_sort Cai, Jin
collection PubMed
description Bacterial ring rot of potato is one of the most serious potato plant and tuber diseases. Laminaria japonica extract was investigated for its antimicrobial activity against Clavibater michiganense subsp. sepedonicum (Spieckermann & Kotthoff) Davis et al., the causative agent of bacterial ring rot of potato. The results showed that the optimum extraction conditions of antimicrobial substances from L. japonica were an extraction temperature of 80°C, an extraction time of 12 h, and a solid to liquid ratio of 1∶25. Active compounds of L. japonica were isolated by solvent partition, thin layer chromatography (TLC) and column chromatography. All nineteen fractionations had antimicrobial activities against C. michiganense subsp. sepedonicum, while Fractionation three (Fr.3) had the highest (P<0.05) antimicrobial activity. Chemical composition analysis identified a total of 26 components in Fr.3. The main constituents of Fr.3 were alkanes (80.97%), esters (5.24%), acids (4.87%) and alcohols (2.21%). Antimicrobial activity of Fr.3 against C. michiganense subsp. sepedonicum could be attributed to its ability to damage the cell wall and cell membrane, induce the production of reactive oxygen species (ROS), increase cytosolic Ca(2+) concentration, inhibit the glycolytic pathway (EMP) and tricarboxylic acid (TCA) cycle, inhibit protein and nucleic acid synthesis, and disrupt the normal cycle of DNA replication. These findings indicate that L. japonica extracts have potential for inhibiting C. michiganense subsp. sepedonicum.
format Online
Article
Text
id pubmed-3979835
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-39798352014-04-11 Laminaria japonica Extract, an Inhibitor of Clavibater michiganense Subsp. Sepedonicum Cai, Jin Feng, Jia Xie, Shulian Wang, Feipeng Xu, Qiufeng PLoS One Research Article Bacterial ring rot of potato is one of the most serious potato plant and tuber diseases. Laminaria japonica extract was investigated for its antimicrobial activity against Clavibater michiganense subsp. sepedonicum (Spieckermann & Kotthoff) Davis et al., the causative agent of bacterial ring rot of potato. The results showed that the optimum extraction conditions of antimicrobial substances from L. japonica were an extraction temperature of 80°C, an extraction time of 12 h, and a solid to liquid ratio of 1∶25. Active compounds of L. japonica were isolated by solvent partition, thin layer chromatography (TLC) and column chromatography. All nineteen fractionations had antimicrobial activities against C. michiganense subsp. sepedonicum, while Fractionation three (Fr.3) had the highest (P<0.05) antimicrobial activity. Chemical composition analysis identified a total of 26 components in Fr.3. The main constituents of Fr.3 were alkanes (80.97%), esters (5.24%), acids (4.87%) and alcohols (2.21%). Antimicrobial activity of Fr.3 against C. michiganense subsp. sepedonicum could be attributed to its ability to damage the cell wall and cell membrane, induce the production of reactive oxygen species (ROS), increase cytosolic Ca(2+) concentration, inhibit the glycolytic pathway (EMP) and tricarboxylic acid (TCA) cycle, inhibit protein and nucleic acid synthesis, and disrupt the normal cycle of DNA replication. These findings indicate that L. japonica extracts have potential for inhibiting C. michiganense subsp. sepedonicum. Public Library of Science 2014-04-08 /pmc/articles/PMC3979835/ /pubmed/24714388 http://dx.doi.org/10.1371/journal.pone.0094329 Text en © 2014 Cai et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Cai, Jin
Feng, Jia
Xie, Shulian
Wang, Feipeng
Xu, Qiufeng
Laminaria japonica Extract, an Inhibitor of Clavibater michiganense Subsp. Sepedonicum
title Laminaria japonica Extract, an Inhibitor of Clavibater michiganense Subsp. Sepedonicum
title_full Laminaria japonica Extract, an Inhibitor of Clavibater michiganense Subsp. Sepedonicum
title_fullStr Laminaria japonica Extract, an Inhibitor of Clavibater michiganense Subsp. Sepedonicum
title_full_unstemmed Laminaria japonica Extract, an Inhibitor of Clavibater michiganense Subsp. Sepedonicum
title_short Laminaria japonica Extract, an Inhibitor of Clavibater michiganense Subsp. Sepedonicum
title_sort laminaria japonica extract, an inhibitor of clavibater michiganense subsp. sepedonicum
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3979835/
https://www.ncbi.nlm.nih.gov/pubmed/24714388
http://dx.doi.org/10.1371/journal.pone.0094329
work_keys_str_mv AT caijin laminariajaponicaextractaninhibitorofclavibatermichiganensesubspsepedonicum
AT fengjia laminariajaponicaextractaninhibitorofclavibatermichiganensesubspsepedonicum
AT xieshulian laminariajaponicaextractaninhibitorofclavibatermichiganensesubspsepedonicum
AT wangfeipeng laminariajaponicaextractaninhibitorofclavibatermichiganensesubspsepedonicum
AT xuqiufeng laminariajaponicaextractaninhibitorofclavibatermichiganensesubspsepedonicum