Characterization of a Canine Tetranucleotide Microsatellite Marker Located in the First Intron of the Tumor Necrosis Factor Alpha Gene

A polymorphic tetranucleotide (GAAT)(n) microsatellite in the first intron of the canine tumor necrosis factor alpha (TNFA) gene was characterized in this study; 139 dogs were analyzed: 22 Beagles, 26 Chihuahuas, 20 Miniature Dachshunds, 24 Miniature Poodles, 22 Pembroke Welsh Corgis and 25 Shiba In...

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Detalles Bibliográficos
Autores principales: WATANABE, Masashi, TANAKA, Kazuaki, TAKIZAWA, Tatsuya, SEGAWA, Kazuhito, NEO, Sakurako, TSUCHIYA, Ryo, MURATA, Michiko, MURAKAMI, Masaru, HISASUE, Masaharu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2013
Materias:
Biochemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3979939/
https://www.ncbi.nlm.nih.gov/pubmed/24042337
http://dx.doi.org/10.1292/jvms.13-0316
Descripción
Sumario:A polymorphic tetranucleotide (GAAT)(n) microsatellite in the first intron of the canine tumor necrosis factor alpha (TNFA) gene was characterized in this study; 139 dogs were analyzed: 22 Beagles, 26 Chihuahuas, 20 Miniature Dachshunds, 24 Miniature Poodles, 22 Pembroke Welsh Corgis and 25 Shiba Inus. We detected the presence of the 4 alleles (GAAT)(5), (GAAT)(6), (GAAT)(7) and (GAAT)(8), including 9 of the 10 expected genotypes. The expected heterozygosity (He) and the polymorphic information content (PIC) value of this microsatellite locus varied from 0.389 to 0.749 and from 0.333 to 0.682, respectively, among the 6 breeds. The allelic frequency differed greatly among breeds, but this microsatellite marker was highly polymorphic and could be a useful marker for the canine TNFA gene.

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