Characterization of a Canine Tetranucleotide Microsatellite Marker Located in the First Intron of the Tumor Necrosis Factor Alpha Gene

A polymorphic tetranucleotide (GAAT)(n) microsatellite in the first intron of the canine tumor necrosis factor alpha (TNFA) gene was characterized in this study; 139 dogs were analyzed: 22 Beagles, 26 Chihuahuas, 20 Miniature Dachshunds, 24 Miniature Poodles, 22 Pembroke Welsh Corgis and 25 Shiba In...

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Autores principales: WATANABE, Masashi, TANAKA, Kazuaki, TAKIZAWA, Tatsuya, SEGAWA, Kazuhito, NEO, Sakurako, TSUCHIYA, Ryo, MURATA, Michiko, MURAKAMI, Masaru, HISASUE, Masaharu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2013
Materias:
Biochemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3979939/
https://www.ncbi.nlm.nih.gov/pubmed/24042337
http://dx.doi.org/10.1292/jvms.13-0316
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author WATANABE, Masashi
TANAKA, Kazuaki
TAKIZAWA, Tatsuya
SEGAWA, Kazuhito
NEO, Sakurako
TSUCHIYA, Ryo
MURATA, Michiko
MURAKAMI, Masaru
HISASUE, Masaharu
author_facet WATANABE, Masashi
TANAKA, Kazuaki
TAKIZAWA, Tatsuya
SEGAWA, Kazuhito
NEO, Sakurako
TSUCHIYA, Ryo
MURATA, Michiko
MURAKAMI, Masaru
HISASUE, Masaharu
author_sort WATANABE, Masashi
collection PubMed
description A polymorphic tetranucleotide (GAAT)(n) microsatellite in the first intron of the canine tumor necrosis factor alpha (TNFA) gene was characterized in this study; 139 dogs were analyzed: 22 Beagles, 26 Chihuahuas, 20 Miniature Dachshunds, 24 Miniature Poodles, 22 Pembroke Welsh Corgis and 25 Shiba Inus. We detected the presence of the 4 alleles (GAAT)(5), (GAAT)(6), (GAAT)(7) and (GAAT)(8), including 9 of the 10 expected genotypes. The expected heterozygosity (He) and the polymorphic information content (PIC) value of this microsatellite locus varied from 0.389 to 0.749 and from 0.333 to 0.682, respectively, among the 6 breeds. The allelic frequency differed greatly among breeds, but this microsatellite marker was highly polymorphic and could be a useful marker for the canine TNFA gene.
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spelling pubmed-39799392014-04-22 Characterization of a Canine Tetranucleotide Microsatellite Marker Located in the First Intron of the Tumor Necrosis Factor Alpha Gene WATANABE, Masashi TANAKA, Kazuaki TAKIZAWA, Tatsuya SEGAWA, Kazuhito NEO, Sakurako TSUCHIYA, Ryo MURATA, Michiko MURAKAMI, Masaru HISASUE, Masaharu J Vet Med Sci Biochemistry A polymorphic tetranucleotide (GAAT)(n) microsatellite in the first intron of the canine tumor necrosis factor alpha (TNFA) gene was characterized in this study; 139 dogs were analyzed: 22 Beagles, 26 Chihuahuas, 20 Miniature Dachshunds, 24 Miniature Poodles, 22 Pembroke Welsh Corgis and 25 Shiba Inus. We detected the presence of the 4 alleles (GAAT)(5), (GAAT)(6), (GAAT)(7) and (GAAT)(8), including 9 of the 10 expected genotypes. The expected heterozygosity (He) and the polymorphic information content (PIC) value of this microsatellite locus varied from 0.389 to 0.749 and from 0.333 to 0.682, respectively, among the 6 breeds. The allelic frequency differed greatly among breeds, but this microsatellite marker was highly polymorphic and could be a useful marker for the canine TNFA gene. The Japanese Society of Veterinary Science 2013-09-13 2014-01 /pmc/articles/PMC3979939/ /pubmed/24042337 http://dx.doi.org/10.1292/jvms.13-0316 Text en ©2014 The Japanese Society of Veterinary Science http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Biochemistry
WATANABE, Masashi
TANAKA, Kazuaki
TAKIZAWA, Tatsuya
SEGAWA, Kazuhito
NEO, Sakurako
TSUCHIYA, Ryo
MURATA, Michiko
MURAKAMI, Masaru
HISASUE, Masaharu
Characterization of a Canine Tetranucleotide Microsatellite Marker Located in the First Intron of the Tumor Necrosis Factor Alpha Gene
title Characterization of a Canine Tetranucleotide Microsatellite Marker Located in the First Intron of the Tumor Necrosis Factor Alpha Gene
title_full Characterization of a Canine Tetranucleotide Microsatellite Marker Located in the First Intron of the Tumor Necrosis Factor Alpha Gene
title_fullStr Characterization of a Canine Tetranucleotide Microsatellite Marker Located in the First Intron of the Tumor Necrosis Factor Alpha Gene
title_full_unstemmed Characterization of a Canine Tetranucleotide Microsatellite Marker Located in the First Intron of the Tumor Necrosis Factor Alpha Gene
title_short Characterization of a Canine Tetranucleotide Microsatellite Marker Located in the First Intron of the Tumor Necrosis Factor Alpha Gene
title_sort characterization of a canine tetranucleotide microsatellite marker located in the first intron of the tumor necrosis factor alpha gene
topic Biochemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3979939/
https://www.ncbi.nlm.nih.gov/pubmed/24042337
http://dx.doi.org/10.1292/jvms.13-0316
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