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Establishment and Application of a Loop-Mediated Isothermal Amplification Method for Simple, Specific, Sensitive and Rapid Detection of Toxoplasma gondii

The Loop-mediated isothermal amplification (LAMP) method amplifies DNA with high simply, specificity, sensitivity and rapidity. In this study, A LAMP assay with 6 primers targeting a highly conserved region of the GRA1 gene was developed to diagnose Toxoplasma gondii. The reaction time of the LAMP a...

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Detalles Bibliográficos
Autores principales: CAO, Lili, CHENG, Ronghua, YAO, Lin, YUAN, Shuxian, YAO, Xinhua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3979957/
https://www.ncbi.nlm.nih.gov/pubmed/23965849
http://dx.doi.org/10.1292/jvms.13-0275