Cargando…

Digoxigenin-Labeled In Situ Hybridization for the Detection of Streptococcus suis DNA in Polyserositis and a Comparison with Biotinylated In Situ Hybridization

The objective of this study was to develop digoxigenin-labeled in situ hybridization (ISH) for the detection of Streptococcus suis in naturally infected pigs with polyserositis and to compare it with biotinylated ISH. Digoxigenin-labeled hybridization signals for S. suis were observed in cells that...

Descripción completa

Detalles Bibliográficos
Autores principales: KANG, Ikjae, SEO, Hwi Won, PARK, Changhoon, OH, Yeonsu, LEE, Jeehoon, YOU, Ok Heui, KIM, Sung-Hoon, GOTTSCHALK, Marcelo, CHAE, Chanhee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3979959/
https://www.ncbi.nlm.nih.gov/pubmed/23985415
http://dx.doi.org/10.1292/jvms.13-0204
_version_ 1782310791627669504
author KANG, Ikjae
SEO, Hwi Won
PARK, Changhoon
OH, Yeonsu
LEE, Jeehoon
YOU, Ok Heui
KIM, Sung-Hoon
GOTTSCHALK, Marcelo
CHAE, Chanhee
author_facet KANG, Ikjae
SEO, Hwi Won
PARK, Changhoon
OH, Yeonsu
LEE, Jeehoon
YOU, Ok Heui
KIM, Sung-Hoon
GOTTSCHALK, Marcelo
CHAE, Chanhee
author_sort KANG, Ikjae
collection PubMed
description The objective of this study was to develop digoxigenin-labeled in situ hybridization (ISH) for the detection of Streptococcus suis in naturally infected pigs with polyserositis and to compare it with biotinylated ISH. Digoxigenin-labeled hybridization signals for S. suis were observed in cells that had infiltrated the fibrous polyserositis and microcolonies in the blood vessels. Mock hybridization showed no hybridization signals for endogenous digoxigenin. Biotinylated hybridization signals for S. suis were observed in cells that had infiltrated the fibrous polyserositis. However, similar hybridization signals were also observed in the fibrous inflammatory area using mock hybridization for endogenous biotin. The present study demonstrated that digoxigenin-labeled ISH is a valuable diagnostic tool for specific detection of S. suis in polyserositic tissues without nonspecific reactions compared with biotinylated ISH.
format Online
Article
Text
id pubmed-3979959
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher The Japanese Society of Veterinary Science
record_format MEDLINE/PubMed
spelling pubmed-39799592014-04-22 Digoxigenin-Labeled In Situ Hybridization for the Detection of Streptococcus suis DNA in Polyserositis and a Comparison with Biotinylated In Situ Hybridization KANG, Ikjae SEO, Hwi Won PARK, Changhoon OH, Yeonsu LEE, Jeehoon YOU, Ok Heui KIM, Sung-Hoon GOTTSCHALK, Marcelo CHAE, Chanhee J Vet Med Sci Pathology The objective of this study was to develop digoxigenin-labeled in situ hybridization (ISH) for the detection of Streptococcus suis in naturally infected pigs with polyserositis and to compare it with biotinylated ISH. Digoxigenin-labeled hybridization signals for S. suis were observed in cells that had infiltrated the fibrous polyserositis and microcolonies in the blood vessels. Mock hybridization showed no hybridization signals for endogenous digoxigenin. Biotinylated hybridization signals for S. suis were observed in cells that had infiltrated the fibrous polyserositis. However, similar hybridization signals were also observed in the fibrous inflammatory area using mock hybridization for endogenous biotin. The present study demonstrated that digoxigenin-labeled ISH is a valuable diagnostic tool for specific detection of S. suis in polyserositic tissues without nonspecific reactions compared with biotinylated ISH. The Japanese Society of Veterinary Science 2013-08-28 2014-01 /pmc/articles/PMC3979959/ /pubmed/23985415 http://dx.doi.org/10.1292/jvms.13-0204 Text en ©2014 The Japanese Society of Veterinary Science http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Pathology
KANG, Ikjae
SEO, Hwi Won
PARK, Changhoon
OH, Yeonsu
LEE, Jeehoon
YOU, Ok Heui
KIM, Sung-Hoon
GOTTSCHALK, Marcelo
CHAE, Chanhee
Digoxigenin-Labeled In Situ Hybridization for the Detection of Streptococcus suis DNA in Polyserositis and a Comparison with Biotinylated In Situ Hybridization
title Digoxigenin-Labeled In Situ Hybridization for the Detection of Streptococcus suis DNA in Polyserositis and a Comparison with Biotinylated In Situ Hybridization
title_full Digoxigenin-Labeled In Situ Hybridization for the Detection of Streptococcus suis DNA in Polyserositis and a Comparison with Biotinylated In Situ Hybridization
title_fullStr Digoxigenin-Labeled In Situ Hybridization for the Detection of Streptococcus suis DNA in Polyserositis and a Comparison with Biotinylated In Situ Hybridization
title_full_unstemmed Digoxigenin-Labeled In Situ Hybridization for the Detection of Streptococcus suis DNA in Polyserositis and a Comparison with Biotinylated In Situ Hybridization
title_short Digoxigenin-Labeled In Situ Hybridization for the Detection of Streptococcus suis DNA in Polyserositis and a Comparison with Biotinylated In Situ Hybridization
title_sort digoxigenin-labeled in situ hybridization for the detection of streptococcus suis dna in polyserositis and a comparison with biotinylated in situ hybridization
topic Pathology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3979959/
https://www.ncbi.nlm.nih.gov/pubmed/23985415
http://dx.doi.org/10.1292/jvms.13-0204
work_keys_str_mv AT kangikjae digoxigeninlabeledinsituhybridizationforthedetectionofstreptococcussuisdnainpolyserositisandacomparisonwithbiotinylatedinsituhybridization
AT seohwiwon digoxigeninlabeledinsituhybridizationforthedetectionofstreptococcussuisdnainpolyserositisandacomparisonwithbiotinylatedinsituhybridization
AT parkchanghoon digoxigeninlabeledinsituhybridizationforthedetectionofstreptococcussuisdnainpolyserositisandacomparisonwithbiotinylatedinsituhybridization
AT ohyeonsu digoxigeninlabeledinsituhybridizationforthedetectionofstreptococcussuisdnainpolyserositisandacomparisonwithbiotinylatedinsituhybridization
AT leejeehoon digoxigeninlabeledinsituhybridizationforthedetectionofstreptococcussuisdnainpolyserositisandacomparisonwithbiotinylatedinsituhybridization
AT youokheui digoxigeninlabeledinsituhybridizationforthedetectionofstreptococcussuisdnainpolyserositisandacomparisonwithbiotinylatedinsituhybridization
AT kimsunghoon digoxigeninlabeledinsituhybridizationforthedetectionofstreptococcussuisdnainpolyserositisandacomparisonwithbiotinylatedinsituhybridization
AT gottschalkmarcelo digoxigeninlabeledinsituhybridizationforthedetectionofstreptococcussuisdnainpolyserositisandacomparisonwithbiotinylatedinsituhybridization
AT chaechanhee digoxigeninlabeledinsituhybridizationforthedetectionofstreptococcussuisdnainpolyserositisandacomparisonwithbiotinylatedinsituhybridization