Cargando…

Tight Association between Macrophages and Adipocytes in Obesity: Implications for Adipocyte Preparation

OBJECTIVE: To determine the cellular architecture of the inflammatory infiltrate in adipose tissue from obese mice, and identify the source of inflammatory cytokines in adipose tissue at a single cell level. DESIGN AND METHODS: Adipose tissue from diet-induced obese mice was digested by collagenase...

Descripción completa

Detalles Bibliográficos
Autores principales: Ebke, Lindsey A., Nestor-Kalinoski, Andrea L., Slotterbeck, Brandon D., Al Dieri, Ali G., Ghosh-Lester, Sumona, Russo, Lucia, Najjar, Sonia M., von Grafenstein, Hermann, McInerney, Marcia F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3980195/
https://www.ncbi.nlm.nih.gov/pubmed/24376179
http://dx.doi.org/10.1002/oby.20634
Descripción
Sumario:OBJECTIVE: To determine the cellular architecture of the inflammatory infiltrate in adipose tissue from obese mice, and identify the source of inflammatory cytokines in adipose tissue at a single cell level. DESIGN AND METHODS: Adipose tissue from diet-induced obese mice was digested by collagenase treatment and fractionated by density centrifugation to obtain an adipocyte floating layer and a pellet of stromal vascular cells. The cellular architecture of the adipocyte-macrophage interaction in both intact white adipose tissue and the separated density gradient floating layer fraction was analyzed by confocal immunohistochemistry. Cytokine expression was detected by semi-quantitative real time PCR and immunohistochemical analysis. RESULTS: Three dimensional image analysis of white adipose tissue and the separated ‘adipocyte’ floating layer revealed lipid-engorged macrophages, macrophages in contact with lipid droplets and sheath-like assemblies of macrophages surrounding adipocytes. The macrophages immunostained for TNFα and to a lesser extent for the immunoregulatory cytokine IL-10. TNFα staining was associated only with macrophages indicating that macrophages and not adipocytes are the source of TNFα expression in the adipocyte floating layer. CONCLUSION: Macrophages form assemblies that tightly adhere to and cover adipocytes and lipid droplets. TNFα found in low density adipocyte preparations is due to contamination with macrophages.