Use of microcarriers in Mobius(® )CellReady bioreactors to support growth of adherent cells

The Mobius(® )CellReady bioreactor product platform incorporates novel disposable technologies that provide optimal performance for suspension mammalian cell culture. Here we show the utility of EMD Millipore's 3L and 50L CellReady single use bioreactors for the cultivation of adherent mammalia...

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Detalles Bibliográficos
Autores principales: McGlothlen, Michael, Jing, Donghui, Martin, Christopher, Phillips, Michael, Shaw, Robert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Poster Presentation
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3980274/
http://dx.doi.org/10.1186/1753-6561-7-S6-P95
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author McGlothlen, Michael
Jing, Donghui
Martin, Christopher
Phillips, Michael
Shaw, Robert
author_facet McGlothlen, Michael
Jing, Donghui
Martin, Christopher
Phillips, Michael
Shaw, Robert
author_sort McGlothlen, Michael
collection PubMed
description The Mobius(® )CellReady bioreactor product platform incorporates novel disposable technologies that provide optimal performance for suspension mammalian cell culture. Here we show the utility of EMD Millipore's 3L and 50L CellReady single use bioreactors for the cultivation of adherent mammalian cells on microcarriers. Cytodex(3)(® )and Solohill(® )collagen microcarriers were first tested in a mixing study to assess feasibility. We evaluated the normalized mixing speed required in the 3L and 50L to achieve a suspension of the microcarriers and enable growth of the cells.
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spelling pubmed-39802742014-04-24 Use of microcarriers in Mobius(® )CellReady bioreactors to support growth of adherent cells McGlothlen, Michael Jing, Donghui Martin, Christopher Phillips, Michael Shaw, Robert BMC Proc Poster Presentation The Mobius(® )CellReady bioreactor product platform incorporates novel disposable technologies that provide optimal performance for suspension mammalian cell culture. Here we show the utility of EMD Millipore's 3L and 50L CellReady single use bioreactors for the cultivation of adherent mammalian cells on microcarriers. Cytodex(3)(® )and Solohill(® )collagen microcarriers were first tested in a mixing study to assess feasibility. We evaluated the normalized mixing speed required in the 3L and 50L to achieve a suspension of the microcarriers and enable growth of the cells. BioMed Central 2013-12-04 /pmc/articles/PMC3980274/ http://dx.doi.org/10.1186/1753-6561-7-S6-P95 Text en Copyright © 2013 McGlothlen et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Poster Presentation
McGlothlen, Michael
Jing, Donghui
Martin, Christopher
Phillips, Michael
Shaw, Robert
Use of microcarriers in Mobius(® )CellReady bioreactors to support growth of adherent cells
title Use of microcarriers in Mobius(® )CellReady bioreactors to support growth of adherent cells
title_full Use of microcarriers in Mobius(® )CellReady bioreactors to support growth of adherent cells
title_fullStr Use of microcarriers in Mobius(® )CellReady bioreactors to support growth of adherent cells
title_full_unstemmed Use of microcarriers in Mobius(® )CellReady bioreactors to support growth of adherent cells
title_short Use of microcarriers in Mobius(® )CellReady bioreactors to support growth of adherent cells
title_sort use of microcarriers in mobius(® )cellready bioreactors to support growth of adherent cells
topic Poster Presentation
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3980274/
http://dx.doi.org/10.1186/1753-6561-7-S6-P95
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