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Label-free in vivo imaging of myelinated axons in health and disease with spectral confocal reflectance microscopy

We report a new technique for high-resolution in vivo imaging of myelinated axons in the brain, spinal cord and peripheral nerve that requires no fluorescent labeling. This method, based on spectral confocal reflectance microscopy (SCoRe), uses a conventional laser scanning confocal system to genera...

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Autores principales: Schain, Aaron J., Hill, Robert A., Grutzendler, Jaime
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3981936/
https://www.ncbi.nlm.nih.gov/pubmed/24681598
http://dx.doi.org/10.1038/nm.3495
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author Schain, Aaron J.
Hill, Robert A.
Grutzendler, Jaime
author_facet Schain, Aaron J.
Hill, Robert A.
Grutzendler, Jaime
author_sort Schain, Aaron J.
collection PubMed
description We report a new technique for high-resolution in vivo imaging of myelinated axons in the brain, spinal cord and peripheral nerve that requires no fluorescent labeling. This method, based on spectral confocal reflectance microscopy (SCoRe), uses a conventional laser scanning confocal system to generate images by merging the simultaneously reflected signals from multiple lasers of different wavelengths. Striking color patterns unique to individual myelinated fibers are generated that facilitate their tracing in dense axonal areas. These patterns highlight nodes of Ranvier and Schmidt-Lanterman incisures and can be used to detect various myelin pathologies. Using SCoRe we performed chronic brain imaging up to 400 μm deep, capturing for the first time de novo myelination of mouse cortical axons in vivo. We also established the feasibility of imaging myelinated axons in the human cerebral cortex. SCoRe adds a powerful component to the evolving toolbox for imaging myelination in living animals and potentially in humans.
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spelling pubmed-39819362014-10-01 Label-free in vivo imaging of myelinated axons in health and disease with spectral confocal reflectance microscopy Schain, Aaron J. Hill, Robert A. Grutzendler, Jaime Nat Med Article We report a new technique for high-resolution in vivo imaging of myelinated axons in the brain, spinal cord and peripheral nerve that requires no fluorescent labeling. This method, based on spectral confocal reflectance microscopy (SCoRe), uses a conventional laser scanning confocal system to generate images by merging the simultaneously reflected signals from multiple lasers of different wavelengths. Striking color patterns unique to individual myelinated fibers are generated that facilitate their tracing in dense axonal areas. These patterns highlight nodes of Ranvier and Schmidt-Lanterman incisures and can be used to detect various myelin pathologies. Using SCoRe we performed chronic brain imaging up to 400 μm deep, capturing for the first time de novo myelination of mouse cortical axons in vivo. We also established the feasibility of imaging myelinated axons in the human cerebral cortex. SCoRe adds a powerful component to the evolving toolbox for imaging myelination in living animals and potentially in humans. 2014-03-30 2014-04 /pmc/articles/PMC3981936/ /pubmed/24681598 http://dx.doi.org/10.1038/nm.3495 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Schain, Aaron J.
Hill, Robert A.
Grutzendler, Jaime
Label-free in vivo imaging of myelinated axons in health and disease with spectral confocal reflectance microscopy
title Label-free in vivo imaging of myelinated axons in health and disease with spectral confocal reflectance microscopy
title_full Label-free in vivo imaging of myelinated axons in health and disease with spectral confocal reflectance microscopy
title_fullStr Label-free in vivo imaging of myelinated axons in health and disease with spectral confocal reflectance microscopy
title_full_unstemmed Label-free in vivo imaging of myelinated axons in health and disease with spectral confocal reflectance microscopy
title_short Label-free in vivo imaging of myelinated axons in health and disease with spectral confocal reflectance microscopy
title_sort label-free in vivo imaging of myelinated axons in health and disease with spectral confocal reflectance microscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3981936/
https://www.ncbi.nlm.nih.gov/pubmed/24681598
http://dx.doi.org/10.1038/nm.3495
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