Simultaneous imaging of neural activity in three dimensions

We introduce a scanless optical method to image neuronal activity in three dimensions simultaneously. Using a spatial light modulator and a custom-designed phase mask, we illuminate and collect light simultaneously from different focal planes and perform calcium imaging of neuronal activity in vitro...

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Detalles Bibliográficos
Autores principales: Quirin, Sean, Jackson, Jesse, Peterka, Darcy S., Yuste, Rafael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2014
Materias:
Neuroscience
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3982072/
https://www.ncbi.nlm.nih.gov/pubmed/24772066
http://dx.doi.org/10.3389/fncir.2014.00029
Descripción
Sumario:We introduce a scanless optical method to image neuronal activity in three dimensions simultaneously. Using a spatial light modulator and a custom-designed phase mask, we illuminate and collect light simultaneously from different focal planes and perform calcium imaging of neuronal activity in vitro and in vivo. This method, combining structured illumination with volume projection imaging, could be used as a technological platform for brain activity mapping.

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