Simultaneous imaging of neural activity in three dimensions

We introduce a scanless optical method to image neuronal activity in three dimensions simultaneously. Using a spatial light modulator and a custom-designed phase mask, we illuminate and collect light simultaneously from different focal planes and perform calcium imaging of neuronal activity in vitro...

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Detalles Bibliográficos
Autores principales: Quirin, Sean, Jackson, Jesse, Peterka, Darcy S., Yuste, Rafael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2014
Materias:
Neuroscience
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3982072/
https://www.ncbi.nlm.nih.gov/pubmed/24772066
http://dx.doi.org/10.3389/fncir.2014.00029
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author Quirin, Sean
Jackson, Jesse
Peterka, Darcy S.
Yuste, Rafael
author_facet Quirin, Sean
Jackson, Jesse
Peterka, Darcy S.
Yuste, Rafael
author_sort Quirin, Sean
collection PubMed
description We introduce a scanless optical method to image neuronal activity in three dimensions simultaneously. Using a spatial light modulator and a custom-designed phase mask, we illuminate and collect light simultaneously from different focal planes and perform calcium imaging of neuronal activity in vitro and in vivo. This method, combining structured illumination with volume projection imaging, could be used as a technological platform for brain activity mapping.
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spelling pubmed-39820722014-04-25 Simultaneous imaging of neural activity in three dimensions Quirin, Sean Jackson, Jesse Peterka, Darcy S. Yuste, Rafael Front Neural Circuits Neuroscience We introduce a scanless optical method to image neuronal activity in three dimensions simultaneously. Using a spatial light modulator and a custom-designed phase mask, we illuminate and collect light simultaneously from different focal planes and perform calcium imaging of neuronal activity in vitro and in vivo. This method, combining structured illumination with volume projection imaging, could be used as a technological platform for brain activity mapping. Frontiers Media S.A. 2014-04-03 /pmc/articles/PMC3982072/ /pubmed/24772066 http://dx.doi.org/10.3389/fncir.2014.00029 Text en Copyright © 2014 Quirin, Jackson, Peterka and Yuste. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Quirin, Sean
Jackson, Jesse
Peterka, Darcy S.
Yuste, Rafael
Simultaneous imaging of neural activity in three dimensions
title Simultaneous imaging of neural activity in three dimensions
title_full Simultaneous imaging of neural activity in three dimensions
title_fullStr Simultaneous imaging of neural activity in three dimensions
title_full_unstemmed Simultaneous imaging of neural activity in three dimensions
title_short Simultaneous imaging of neural activity in three dimensions
title_sort simultaneous imaging of neural activity in three dimensions
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3982072/
https://www.ncbi.nlm.nih.gov/pubmed/24772066
http://dx.doi.org/10.3389/fncir.2014.00029
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AT jacksonjesse simultaneousimagingofneuralactivityinthreedimensions
AT peterkadarcys simultaneousimagingofneuralactivityinthreedimensions
AT yusterafael simultaneousimagingofneuralactivityinthreedimensions

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