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Polyglycerolsulfate Functionalized Gold Nanorods as Optoacoustic Signal Nanoamplifiers for In Vivo Bioimaging of Rheumatoid Arthritis

We have synthesized a targeted imaging agent for rheumatoid arthritis based on polysulfated gold nanorods. The CTAB layer on gold nanorods was first replaced with PEG-thiol and then with dendritic polyglycerolsulfate at elevated temperature, which resulted in significantly reduced cytotoxicity compa...

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Autores principales: Vonnemann, Jonathan, Beziere, Nicolas, Böttcher, Christoph, Riese, Sebastian B., Kuehne, Christian, Dernedde, Jens, Licha, Kai, von Schacky, Claudio, Kosanke, Yvonne, Kimm, Melanie, Meier, Reinhard, Ntziachristos, Vasilis, Haag, Rainer
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3982133/
https://www.ncbi.nlm.nih.gov/pubmed/24723984
http://dx.doi.org/10.7150/thno.8518
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author Vonnemann, Jonathan
Beziere, Nicolas
Böttcher, Christoph
Riese, Sebastian B.
Kuehne, Christian
Dernedde, Jens
Licha, Kai
von Schacky, Claudio
Kosanke, Yvonne
Kimm, Melanie
Meier, Reinhard
Ntziachristos, Vasilis
Haag, Rainer
author_facet Vonnemann, Jonathan
Beziere, Nicolas
Böttcher, Christoph
Riese, Sebastian B.
Kuehne, Christian
Dernedde, Jens
Licha, Kai
von Schacky, Claudio
Kosanke, Yvonne
Kimm, Melanie
Meier, Reinhard
Ntziachristos, Vasilis
Haag, Rainer
author_sort Vonnemann, Jonathan
collection PubMed
description We have synthesized a targeted imaging agent for rheumatoid arthritis based on polysulfated gold nanorods. The CTAB layer on gold nanorods was first replaced with PEG-thiol and then with dendritic polyglycerolsulfate at elevated temperature, which resulted in significantly reduced cytotoxicity compared to polyanionic gold nanorods functionalized by non-covalent approaches. In addition to classical characterization methods, we have established a facile UV-VIS based BaCl(2) agglomeration assay to confirm a quantitative removal of unbound ligand. With the help of a competitive surface plasmon resonance-based L-selectin binding assay and a leukocyte adhesion-based flow cell assay, we have demonstrated the high inflammation targeting potential of the synthesized gold nanorods in vitro. In combination with the surface plasmon resonance band of AuNRs at 780 nm, these findings permitted the imaging of inflammation in an in vivo mouse model for rheumatoid arthritis with high contrast using multispectral optoacoustic tomography. The study offers a robust method for otherwise difficult to obtain covalently functionalized polyanionic gold nanorods, which are suitable for biological applications as well as a low-cost, actively targeted, and high contrast imaging agent for the diagnosis of rheumatoid arthritis. This paves the way for further research in other inflammation associated pathologies, in particular, when photothermal therapy can be applied.
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spelling pubmed-39821332014-04-10 Polyglycerolsulfate Functionalized Gold Nanorods as Optoacoustic Signal Nanoamplifiers for In Vivo Bioimaging of Rheumatoid Arthritis Vonnemann, Jonathan Beziere, Nicolas Böttcher, Christoph Riese, Sebastian B. Kuehne, Christian Dernedde, Jens Licha, Kai von Schacky, Claudio Kosanke, Yvonne Kimm, Melanie Meier, Reinhard Ntziachristos, Vasilis Haag, Rainer Theranostics Research Paper We have synthesized a targeted imaging agent for rheumatoid arthritis based on polysulfated gold nanorods. The CTAB layer on gold nanorods was first replaced with PEG-thiol and then with dendritic polyglycerolsulfate at elevated temperature, which resulted in significantly reduced cytotoxicity compared to polyanionic gold nanorods functionalized by non-covalent approaches. In addition to classical characterization methods, we have established a facile UV-VIS based BaCl(2) agglomeration assay to confirm a quantitative removal of unbound ligand. With the help of a competitive surface plasmon resonance-based L-selectin binding assay and a leukocyte adhesion-based flow cell assay, we have demonstrated the high inflammation targeting potential of the synthesized gold nanorods in vitro. In combination with the surface plasmon resonance band of AuNRs at 780 nm, these findings permitted the imaging of inflammation in an in vivo mouse model for rheumatoid arthritis with high contrast using multispectral optoacoustic tomography. The study offers a robust method for otherwise difficult to obtain covalently functionalized polyanionic gold nanorods, which are suitable for biological applications as well as a low-cost, actively targeted, and high contrast imaging agent for the diagnosis of rheumatoid arthritis. This paves the way for further research in other inflammation associated pathologies, in particular, when photothermal therapy can be applied. Ivyspring International Publisher 2014-03-20 /pmc/articles/PMC3982133/ /pubmed/24723984 http://dx.doi.org/10.7150/thno.8518 Text en © Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.
spellingShingle Research Paper
Vonnemann, Jonathan
Beziere, Nicolas
Böttcher, Christoph
Riese, Sebastian B.
Kuehne, Christian
Dernedde, Jens
Licha, Kai
von Schacky, Claudio
Kosanke, Yvonne
Kimm, Melanie
Meier, Reinhard
Ntziachristos, Vasilis
Haag, Rainer
Polyglycerolsulfate Functionalized Gold Nanorods as Optoacoustic Signal Nanoamplifiers for In Vivo Bioimaging of Rheumatoid Arthritis
title Polyglycerolsulfate Functionalized Gold Nanorods as Optoacoustic Signal Nanoamplifiers for In Vivo Bioimaging of Rheumatoid Arthritis
title_full Polyglycerolsulfate Functionalized Gold Nanorods as Optoacoustic Signal Nanoamplifiers for In Vivo Bioimaging of Rheumatoid Arthritis
title_fullStr Polyglycerolsulfate Functionalized Gold Nanorods as Optoacoustic Signal Nanoamplifiers for In Vivo Bioimaging of Rheumatoid Arthritis
title_full_unstemmed Polyglycerolsulfate Functionalized Gold Nanorods as Optoacoustic Signal Nanoamplifiers for In Vivo Bioimaging of Rheumatoid Arthritis
title_short Polyglycerolsulfate Functionalized Gold Nanorods as Optoacoustic Signal Nanoamplifiers for In Vivo Bioimaging of Rheumatoid Arthritis
title_sort polyglycerolsulfate functionalized gold nanorods as optoacoustic signal nanoamplifiers for in vivo bioimaging of rheumatoid arthritis
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3982133/
https://www.ncbi.nlm.nih.gov/pubmed/24723984
http://dx.doi.org/10.7150/thno.8518
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