Optimization of Fluorometric Assay for Ozone in Solution

Ozone is widely used for sterilization, deodorization, and cleaning. However, the effects of ozone on human are not well understood, because there is currently no reliable assay method for ozone. Therefore the accurate measurement of ozone is important for its safe use. Here, we report optimized con...

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Detalles Bibliográficos
Autores principales: Nagano, Hiromi, Shiota, Gotaro, Arakawa, Hidetoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3982405/
https://www.ncbi.nlm.nih.gov/pubmed/24790771
http://dx.doi.org/10.1155/2014/190937
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author Nagano, Hiromi
Shiota, Gotaro
Arakawa, Hidetoshi
author_facet Nagano, Hiromi
Shiota, Gotaro
Arakawa, Hidetoshi
author_sort Nagano, Hiromi
collection PubMed
description Ozone is widely used for sterilization, deodorization, and cleaning. However, the effects of ozone on human are not well understood, because there is currently no reliable assay method for ozone. Therefore the accurate measurement of ozone is important for its safe use. Here, we report optimized conditions for use with fluorescein derivative 1 (probe 1). Under optimum conditions, probe 1 reacted with ozone quantitatively and specifically to generate a fluorescent substance with an excitation maximum at 493 nm and an emission maximum at 523 nm. We conclude that this assay will be useful for quantifying ozone in solution.
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spelling pubmed-39824052014-04-30 Optimization of Fluorometric Assay for Ozone in Solution Nagano, Hiromi Shiota, Gotaro Arakawa, Hidetoshi J Anal Methods Chem Research Article Ozone is widely used for sterilization, deodorization, and cleaning. However, the effects of ozone on human are not well understood, because there is currently no reliable assay method for ozone. Therefore the accurate measurement of ozone is important for its safe use. Here, we report optimized conditions for use with fluorescein derivative 1 (probe 1). Under optimum conditions, probe 1 reacted with ozone quantitatively and specifically to generate a fluorescent substance with an excitation maximum at 493 nm and an emission maximum at 523 nm. We conclude that this assay will be useful for quantifying ozone in solution. Hindawi Publishing Corporation 2014 2014-03-24 /pmc/articles/PMC3982405/ /pubmed/24790771 http://dx.doi.org/10.1155/2014/190937 Text en Copyright © 2014 Hiromi Nagano et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Nagano, Hiromi
Shiota, Gotaro
Arakawa, Hidetoshi
Optimization of Fluorometric Assay for Ozone in Solution
title Optimization of Fluorometric Assay for Ozone in Solution
title_full Optimization of Fluorometric Assay for Ozone in Solution
title_fullStr Optimization of Fluorometric Assay for Ozone in Solution
title_full_unstemmed Optimization of Fluorometric Assay for Ozone in Solution
title_short Optimization of Fluorometric Assay for Ozone in Solution
title_sort optimization of fluorometric assay for ozone in solution
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3982405/
https://www.ncbi.nlm.nih.gov/pubmed/24790771
http://dx.doi.org/10.1155/2014/190937
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