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Characterization of Silk Fibroin Modified Surface: A Proteomic View of Cellular Response Proteins Induced by Biomaterials
The purpose of this study was to develop the pathway of silk fibroin (SF) biopolymer surface induced cell membrane protein activation. Fibroblasts were used as an experimental model to evaluate the responses of cellular proteins induced by biopolymer material using a mass spectrometry-based profilin...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3982454/ https://www.ncbi.nlm.nih.gov/pubmed/24818131 http://dx.doi.org/10.1155/2014/209469 |
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author | Yang, Ming-Hui Yuan, Shyng-Shiou Chung, Tze-Wen Jong, Shiang-Bin Lu, Chi-Yu Tsai, Wan-Chi Chen, Wen-Cheng Lin, Po-Chiao Chiang, Pei-Wen Tyan, Yu-Chang |
author_facet | Yang, Ming-Hui Yuan, Shyng-Shiou Chung, Tze-Wen Jong, Shiang-Bin Lu, Chi-Yu Tsai, Wan-Chi Chen, Wen-Cheng Lin, Po-Chiao Chiang, Pei-Wen Tyan, Yu-Chang |
author_sort | Yang, Ming-Hui |
collection | PubMed |
description | The purpose of this study was to develop the pathway of silk fibroin (SF) biopolymer surface induced cell membrane protein activation. Fibroblasts were used as an experimental model to evaluate the responses of cellular proteins induced by biopolymer material using a mass spectrometry-based profiling system. The surface was covered by multiwalled carbon nanotubes (CNTs) and SF to increase the surface area, enhance the adhesion of biopolymer, and promote the rate of cell proliferation. The amount of adhered fibroblasts on CNTs/SF electrodes of quartz crystal microbalance (QCM) greatly exceeded those on other surfaces. Moreover, analyzing differential protein expressions of adhered fibroblasts on the biopolymer surface by proteomic approaches indicated that CD44 may be a key protein. Through this study, utilization of mass spectrometry-based proteomics in evaluation of cell adhesion on biopolymer was proposed. |
format | Online Article Text |
id | pubmed-3982454 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-39824542014-05-11 Characterization of Silk Fibroin Modified Surface: A Proteomic View of Cellular Response Proteins Induced by Biomaterials Yang, Ming-Hui Yuan, Shyng-Shiou Chung, Tze-Wen Jong, Shiang-Bin Lu, Chi-Yu Tsai, Wan-Chi Chen, Wen-Cheng Lin, Po-Chiao Chiang, Pei-Wen Tyan, Yu-Chang Biomed Res Int Research Article The purpose of this study was to develop the pathway of silk fibroin (SF) biopolymer surface induced cell membrane protein activation. Fibroblasts were used as an experimental model to evaluate the responses of cellular proteins induced by biopolymer material using a mass spectrometry-based profiling system. The surface was covered by multiwalled carbon nanotubes (CNTs) and SF to increase the surface area, enhance the adhesion of biopolymer, and promote the rate of cell proliferation. The amount of adhered fibroblasts on CNTs/SF electrodes of quartz crystal microbalance (QCM) greatly exceeded those on other surfaces. Moreover, analyzing differential protein expressions of adhered fibroblasts on the biopolymer surface by proteomic approaches indicated that CD44 may be a key protein. Through this study, utilization of mass spectrometry-based proteomics in evaluation of cell adhesion on biopolymer was proposed. Hindawi Publishing Corporation 2014 2014-03-25 /pmc/articles/PMC3982454/ /pubmed/24818131 http://dx.doi.org/10.1155/2014/209469 Text en Copyright © 2014 Ming-Hui Yang et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Yang, Ming-Hui Yuan, Shyng-Shiou Chung, Tze-Wen Jong, Shiang-Bin Lu, Chi-Yu Tsai, Wan-Chi Chen, Wen-Cheng Lin, Po-Chiao Chiang, Pei-Wen Tyan, Yu-Chang Characterization of Silk Fibroin Modified Surface: A Proteomic View of Cellular Response Proteins Induced by Biomaterials |
title | Characterization of Silk Fibroin Modified Surface: A Proteomic View of Cellular Response Proteins Induced by Biomaterials |
title_full | Characterization of Silk Fibroin Modified Surface: A Proteomic View of Cellular Response Proteins Induced by Biomaterials |
title_fullStr | Characterization of Silk Fibroin Modified Surface: A Proteomic View of Cellular Response Proteins Induced by Biomaterials |
title_full_unstemmed | Characterization of Silk Fibroin Modified Surface: A Proteomic View of Cellular Response Proteins Induced by Biomaterials |
title_short | Characterization of Silk Fibroin Modified Surface: A Proteomic View of Cellular Response Proteins Induced by Biomaterials |
title_sort | characterization of silk fibroin modified surface: a proteomic view of cellular response proteins induced by biomaterials |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3982454/ https://www.ncbi.nlm.nih.gov/pubmed/24818131 http://dx.doi.org/10.1155/2014/209469 |
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