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Characterization of Silk Fibroin Modified Surface: A Proteomic View of Cellular Response Proteins Induced by Biomaterials

The purpose of this study was to develop the pathway of silk fibroin (SF) biopolymer surface induced cell membrane protein activation. Fibroblasts were used as an experimental model to evaluate the responses of cellular proteins induced by biopolymer material using a mass spectrometry-based profilin...

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Autores principales: Yang, Ming-Hui, Yuan, Shyng-Shiou, Chung, Tze-Wen, Jong, Shiang-Bin, Lu, Chi-Yu, Tsai, Wan-Chi, Chen, Wen-Cheng, Lin, Po-Chiao, Chiang, Pei-Wen, Tyan, Yu-Chang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3982454/
https://www.ncbi.nlm.nih.gov/pubmed/24818131
http://dx.doi.org/10.1155/2014/209469
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author Yang, Ming-Hui
Yuan, Shyng-Shiou
Chung, Tze-Wen
Jong, Shiang-Bin
Lu, Chi-Yu
Tsai, Wan-Chi
Chen, Wen-Cheng
Lin, Po-Chiao
Chiang, Pei-Wen
Tyan, Yu-Chang
author_facet Yang, Ming-Hui
Yuan, Shyng-Shiou
Chung, Tze-Wen
Jong, Shiang-Bin
Lu, Chi-Yu
Tsai, Wan-Chi
Chen, Wen-Cheng
Lin, Po-Chiao
Chiang, Pei-Wen
Tyan, Yu-Chang
author_sort Yang, Ming-Hui
collection PubMed
description The purpose of this study was to develop the pathway of silk fibroin (SF) biopolymer surface induced cell membrane protein activation. Fibroblasts were used as an experimental model to evaluate the responses of cellular proteins induced by biopolymer material using a mass spectrometry-based profiling system. The surface was covered by multiwalled carbon nanotubes (CNTs) and SF to increase the surface area, enhance the adhesion of biopolymer, and promote the rate of cell proliferation. The amount of adhered fibroblasts on CNTs/SF electrodes of quartz crystal microbalance (QCM) greatly exceeded those on other surfaces. Moreover, analyzing differential protein expressions of adhered fibroblasts on the biopolymer surface by proteomic approaches indicated that CD44 may be a key protein. Through this study, utilization of mass spectrometry-based proteomics in evaluation of cell adhesion on biopolymer was proposed.
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spelling pubmed-39824542014-05-11 Characterization of Silk Fibroin Modified Surface: A Proteomic View of Cellular Response Proteins Induced by Biomaterials Yang, Ming-Hui Yuan, Shyng-Shiou Chung, Tze-Wen Jong, Shiang-Bin Lu, Chi-Yu Tsai, Wan-Chi Chen, Wen-Cheng Lin, Po-Chiao Chiang, Pei-Wen Tyan, Yu-Chang Biomed Res Int Research Article The purpose of this study was to develop the pathway of silk fibroin (SF) biopolymer surface induced cell membrane protein activation. Fibroblasts were used as an experimental model to evaluate the responses of cellular proteins induced by biopolymer material using a mass spectrometry-based profiling system. The surface was covered by multiwalled carbon nanotubes (CNTs) and SF to increase the surface area, enhance the adhesion of biopolymer, and promote the rate of cell proliferation. The amount of adhered fibroblasts on CNTs/SF electrodes of quartz crystal microbalance (QCM) greatly exceeded those on other surfaces. Moreover, analyzing differential protein expressions of adhered fibroblasts on the biopolymer surface by proteomic approaches indicated that CD44 may be a key protein. Through this study, utilization of mass spectrometry-based proteomics in evaluation of cell adhesion on biopolymer was proposed. Hindawi Publishing Corporation 2014 2014-03-25 /pmc/articles/PMC3982454/ /pubmed/24818131 http://dx.doi.org/10.1155/2014/209469 Text en Copyright © 2014 Ming-Hui Yang et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Yang, Ming-Hui
Yuan, Shyng-Shiou
Chung, Tze-Wen
Jong, Shiang-Bin
Lu, Chi-Yu
Tsai, Wan-Chi
Chen, Wen-Cheng
Lin, Po-Chiao
Chiang, Pei-Wen
Tyan, Yu-Chang
Characterization of Silk Fibroin Modified Surface: A Proteomic View of Cellular Response Proteins Induced by Biomaterials
title Characterization of Silk Fibroin Modified Surface: A Proteomic View of Cellular Response Proteins Induced by Biomaterials
title_full Characterization of Silk Fibroin Modified Surface: A Proteomic View of Cellular Response Proteins Induced by Biomaterials
title_fullStr Characterization of Silk Fibroin Modified Surface: A Proteomic View of Cellular Response Proteins Induced by Biomaterials
title_full_unstemmed Characterization of Silk Fibroin Modified Surface: A Proteomic View of Cellular Response Proteins Induced by Biomaterials
title_short Characterization of Silk Fibroin Modified Surface: A Proteomic View of Cellular Response Proteins Induced by Biomaterials
title_sort characterization of silk fibroin modified surface: a proteomic view of cellular response proteins induced by biomaterials
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3982454/
https://www.ncbi.nlm.nih.gov/pubmed/24818131
http://dx.doi.org/10.1155/2014/209469
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