Primer Based Approach for PCR Amplification of High GC Content Gene: Mycobacterium Gene as a Model

The genome of Mycobacterium is rich in GC content and poses problem in amplification of some genes, especially those rich in the GC content in terminal regions, by standard/routine PCR procedures. Attempts have been made to amplify three GC rich genes of Mycobacterium sp. (Rv0519c and Rv0774c from M...

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Detalles Bibliográficos
Autores principales: Kumar, Arbind, Kaur, Jagdeep
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3982478/
https://www.ncbi.nlm.nih.gov/pubmed/24782926
http://dx.doi.org/10.1155/2014/937308

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3982478/
https://www.ncbi.nlm.nih.gov/pubmed/24782926
http://dx.doi.org/10.1155/2014/937308

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