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Primer Based Approach for PCR Amplification of High GC Content Gene: Mycobacterium Gene as a Model
The genome of Mycobacterium is rich in GC content and poses problem in amplification of some genes, especially those rich in the GC content in terminal regions, by standard/routine PCR procedures. Attempts have been made to amplify three GC rich genes of Mycobacterium sp. (Rv0519c and Rv0774c from M...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Hindawi Publishing Corporation
2014
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3982478/ https://www.ncbi.nlm.nih.gov/pubmed/24782926 http://dx.doi.org/10.1155/2014/937308 |