Erythrophagocytosis Enhances Heme-Dependent Cytotoxicity of Antimalarial Drugs in Canine Histiocytic Sarcoma Cell Line DH82

Antimalarial drugs, dihydroartemisinin (DHA) and artesunate (ATS), exhibit iron-dependent cytotoxicity in tumor cells. We hypothesized that erythrophagocytic uptake of heme-iron enhances the cytotoxicity of DHA and ATS. Erythrophagocytic (EP) treatment of the canine histiocytic sarcoma cell line DH8...

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Autores principales: CHIKAZAWA, Seishiro, KITAHARA, Yasunori, ANDO, Erika, HORI, Yasutomo, HOSHI, Fumio, KANAI, Kazutaka, ITO, Naoyuki, HIGUCHI, Seiichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2013
Materias:
Internal Medicine
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3982809/
https://www.ncbi.nlm.nih.gov/pubmed/24065080
http://dx.doi.org/10.1292/jvms.13-0319
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author CHIKAZAWA, Seishiro
KITAHARA, Yasunori
ANDO, Erika
HORI, Yasutomo
HOSHI, Fumio
KANAI, Kazutaka
ITO, Naoyuki
HIGUCHI, Seiichi
author_facet CHIKAZAWA, Seishiro
KITAHARA, Yasunori
ANDO, Erika
HORI, Yasutomo
HOSHI, Fumio
KANAI, Kazutaka
ITO, Naoyuki
HIGUCHI, Seiichi
author_sort CHIKAZAWA, Seishiro
collection PubMed
description Antimalarial drugs, dihydroartemisinin (DHA) and artesunate (ATS), exhibit iron-dependent cytotoxicity in tumor cells. We hypothesized that erythrophagocytic uptake of heme-iron enhances the cytotoxicity of DHA and ATS. Erythrophagocytic (EP) treatment of the canine histiocytic sarcoma cell line DH82 markedly increased the cytotoxicity of DHA and ATS compared to controls. Succinyl acetone, an inhibitor of intracellular heme synthesis, decreased the cytotoxicity of DHA and ATS in normal cells, but this change was not observed in EP cells. These results suggest that exogenous heme derived from erythrocytes can enhance the cytotoxicity of DHA and ATS. Furthermore, our study suggests that heme could be a novel component of tumor treatment in veterinary medicine.
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spelling pubmed-39828092014-04-22 Erythrophagocytosis Enhances Heme-Dependent Cytotoxicity of Antimalarial Drugs in Canine Histiocytic Sarcoma Cell Line DH82 CHIKAZAWA, Seishiro KITAHARA, Yasunori ANDO, Erika HORI, Yasutomo HOSHI, Fumio KANAI, Kazutaka ITO, Naoyuki HIGUCHI, Seiichi J Vet Med Sci Internal Medicine Antimalarial drugs, dihydroartemisinin (DHA) and artesunate (ATS), exhibit iron-dependent cytotoxicity in tumor cells. We hypothesized that erythrophagocytic uptake of heme-iron enhances the cytotoxicity of DHA and ATS. Erythrophagocytic (EP) treatment of the canine histiocytic sarcoma cell line DH82 markedly increased the cytotoxicity of DHA and ATS compared to controls. Succinyl acetone, an inhibitor of intracellular heme synthesis, decreased the cytotoxicity of DHA and ATS in normal cells, but this change was not observed in EP cells. These results suggest that exogenous heme derived from erythrocytes can enhance the cytotoxicity of DHA and ATS. Furthermore, our study suggests that heme could be a novel component of tumor treatment in veterinary medicine. The Japanese Society of Veterinary Science 2013-09-20 2014-02 /pmc/articles/PMC3982809/ /pubmed/24065080 http://dx.doi.org/10.1292/jvms.13-0319 Text en ©2014 The Japanese Society of Veterinary Science http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Internal Medicine
CHIKAZAWA, Seishiro
KITAHARA, Yasunori
ANDO, Erika
HORI, Yasutomo
HOSHI, Fumio
KANAI, Kazutaka
ITO, Naoyuki
HIGUCHI, Seiichi
Erythrophagocytosis Enhances Heme-Dependent Cytotoxicity of Antimalarial Drugs in Canine Histiocytic Sarcoma Cell Line DH82
title Erythrophagocytosis Enhances Heme-Dependent Cytotoxicity of Antimalarial Drugs in Canine Histiocytic Sarcoma Cell Line DH82
title_full Erythrophagocytosis Enhances Heme-Dependent Cytotoxicity of Antimalarial Drugs in Canine Histiocytic Sarcoma Cell Line DH82
title_fullStr Erythrophagocytosis Enhances Heme-Dependent Cytotoxicity of Antimalarial Drugs in Canine Histiocytic Sarcoma Cell Line DH82
title_full_unstemmed Erythrophagocytosis Enhances Heme-Dependent Cytotoxicity of Antimalarial Drugs in Canine Histiocytic Sarcoma Cell Line DH82
title_short Erythrophagocytosis Enhances Heme-Dependent Cytotoxicity of Antimalarial Drugs in Canine Histiocytic Sarcoma Cell Line DH82
title_sort erythrophagocytosis enhances heme-dependent cytotoxicity of antimalarial drugs in canine histiocytic sarcoma cell line dh82
topic Internal Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3982809/
https://www.ncbi.nlm.nih.gov/pubmed/24065080
http://dx.doi.org/10.1292/jvms.13-0319
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