Developing an Indirect ELISA Based on Recombinant Hexon Protein for Serological Detection of Inclusion Body Hepatitis in Chickens

Fowl adenovirus (FAdv) serotype 2 causes inclusion body hepatitis (IBH) disease which adversely affects the broiler industry in Thailand. We developed an indirect ELISA based on the recombinant hexon protein produced by E. coli. The recombinant hexon protein was tested with sera, in both infected an...

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Autores principales: JUNNU, Sucheeva, LERTWATCHARASARAKUL, Preeda, JALA, Siriluk, PHATTANAKUNANAN, Sakuna, MOONJIT, Pattra, SONGSERM, Thaweesak
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2013
Materias:
Virology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3982830/
https://www.ncbi.nlm.nih.gov/pubmed/24141320
http://dx.doi.org/10.1292/jvms.13-0196
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author JUNNU, Sucheeva
LERTWATCHARASARAKUL, Preeda
JALA, Siriluk
PHATTANAKUNANAN, Sakuna
MOONJIT, Pattra
SONGSERM, Thaweesak
author_facet JUNNU, Sucheeva
LERTWATCHARASARAKUL, Preeda
JALA, Siriluk
PHATTANAKUNANAN, Sakuna
MOONJIT, Pattra
SONGSERM, Thaweesak
author_sort JUNNU, Sucheeva
collection PubMed
description Fowl adenovirus (FAdv) serotype 2 causes inclusion body hepatitis (IBH) disease which adversely affects the broiler industry in Thailand. We developed an indirect ELISA based on the recombinant hexon protein produced by E. coli. The recombinant hexon protein was tested with sera, in both infected and noninfected chickens. The recombinant hexon protein was standardized with an antigen concentration of 3.75 µg/ml and test sera. The intra- and inter-assays were repeatable. The cutoff value from TG-ROC curve analysis was 0.106. The specificity and sensitivity were 80 and 80%, respectively. The correlation coefficient (r) of absorbance values from this ELISA compared with the serum neutralization test was 0.76. This ELISA might be helpful for IBH diagnosis and surveillance.
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spelling pubmed-39828302014-04-22 Developing an Indirect ELISA Based on Recombinant Hexon Protein for Serological Detection of Inclusion Body Hepatitis in Chickens JUNNU, Sucheeva LERTWATCHARASARAKUL, Preeda JALA, Siriluk PHATTANAKUNANAN, Sakuna MOONJIT, Pattra SONGSERM, Thaweesak J Vet Med Sci Virology Fowl adenovirus (FAdv) serotype 2 causes inclusion body hepatitis (IBH) disease which adversely affects the broiler industry in Thailand. We developed an indirect ELISA based on the recombinant hexon protein produced by E. coli. The recombinant hexon protein was tested with sera, in both infected and noninfected chickens. The recombinant hexon protein was standardized with an antigen concentration of 3.75 µg/ml and test sera. The intra- and inter-assays were repeatable. The cutoff value from TG-ROC curve analysis was 0.106. The specificity and sensitivity were 80 and 80%, respectively. The correlation coefficient (r) of absorbance values from this ELISA compared with the serum neutralization test was 0.76. This ELISA might be helpful for IBH diagnosis and surveillance. The Japanese Society of Veterinary Science 2013-10-21 2014-02 /pmc/articles/PMC3982830/ /pubmed/24141320 http://dx.doi.org/10.1292/jvms.13-0196 Text en ©2014 The Japanese Society of Veterinary Science http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Virology
JUNNU, Sucheeva
LERTWATCHARASARAKUL, Preeda
JALA, Siriluk
PHATTANAKUNANAN, Sakuna
MOONJIT, Pattra
SONGSERM, Thaweesak
Developing an Indirect ELISA Based on Recombinant Hexon Protein for Serological Detection of Inclusion Body Hepatitis in Chickens
title Developing an Indirect ELISA Based on Recombinant Hexon Protein for Serological Detection of Inclusion Body Hepatitis in Chickens
title_full Developing an Indirect ELISA Based on Recombinant Hexon Protein for Serological Detection of Inclusion Body Hepatitis in Chickens
title_fullStr Developing an Indirect ELISA Based on Recombinant Hexon Protein for Serological Detection of Inclusion Body Hepatitis in Chickens
title_full_unstemmed Developing an Indirect ELISA Based on Recombinant Hexon Protein for Serological Detection of Inclusion Body Hepatitis in Chickens
title_short Developing an Indirect ELISA Based on Recombinant Hexon Protein for Serological Detection of Inclusion Body Hepatitis in Chickens
title_sort developing an indirect elisa based on recombinant hexon protein for serological detection of inclusion body hepatitis in chickens
topic Virology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3982830/
https://www.ncbi.nlm.nih.gov/pubmed/24141320
http://dx.doi.org/10.1292/jvms.13-0196
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